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In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture

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Abstract

Micropropagation of the endangered terrestrial orchid, Geodorum densiflorum (Lam.) Schltr. was achieved through rhizome section culture from in vitro seed- derived rhizomes. Rhizome sections were cultured on Murashige and Skoog (MS) and Knudson C(KC) media supplemented with various growth regulators and 0.1% activated charcoal. The rhizome sections responded on MS medium. Naphthaleneacetic acid (NAA) at 2.0 μM stimulated rhizome growth. However, benzyladenine (BA) at 5.0 μM induced multiple shoots within four weeks of culture and inhibited rhizome growth. The regenerated shoots rooted on MS only or with NAA at 1.0 μM. Well-developed plantlets were transferred to community pots and then to a greenhouse where the plants have been acclimatised.

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Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Botany, Karnatak University, Dharwad, 580003, India

    S.S. Sheelavantmath, H.N. Murthy, H.N. Murthy, A.N. Pyati, H.G. Ashok Kumar & B.V. Ravishankar

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  1. S.S. Sheelavantmath
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  2. H.N. Murthy
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  3. A.N. Pyati
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  4. H.G. Ashok Kumar
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  5. B.V. Ravishankar
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Correspondence to H.N. Murthy.

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Sheelavantmath, S., Murthy, H., Pyati, A. et al. In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell, Tissue and Organ Culture 60, 151–154 (2000). https://doi.org/10.1023/A:1006426905052

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  • DOI: https://doi.org/10.1023/A:1006426905052

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