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Identification of a sucrose nonfermenting-1-related protein kinase in sugar beet (Beta vulgaris L.)

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Abstract

A 154 bp polymerase chain reaction product, SBKIN154, showing 76–83% sequence identity with sucrose nonfermenting-1 (SNF1)-related protein kinase nucleotide sequences from other plant species was amplified from sugar beet storage root RNA. Southern blot analysis using SBKIN154 as a hybridisation probe suggested that sugar beet contains either a single-copy SNF1-related gene or a small gene family of highly conserved genes. An antibody raised to a heterologously-expressed fusion of the rye SNF1-related protein kinase, RKIN1, and maltose binding protein, recognised a protein of the expected size (Mr approx. 60,000) on western blots of storage root, stalk, leaf and root extracts. Measurements of SNF1-related activity were made using a specific peptide (SAMS) phosphorylation assay. Activity was highest (0.38 nmol min-1 mg-1 protein) in developing storage roots and lowest (0.035 nmol min-1 mg-1) in fibrous roots.

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References

  1. Alderson A, Sabelli PA, Dickinson JR, Cole D, Richardson M, Kreis M, Shewry PR and Halford NG (1991) Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein kinase cDNA. Proc Natl Acad Sci USA 88: 8602–8605

    Google Scholar 

  2. Ball KL, Barker JHA, Halford NG and Hardie DG (1995) Immunological evidence that HMG-CoA reductase kinase-A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases. FEBS Letts 377: 189–192

    Google Scholar 

  3. Ball KL, Dale S, Weekes J and Hardie DG (1994) Biochemical characterization of two forms of 3-hydroxy-3-methyl-glutaryl CoA from cauliflower (Brassica oleracea). Eur J Biochem 219: 743–750

    Google Scholar 

  4. Barker JHA, Hardie DG, Ball KL, Shewry PR and Halford NG (1996) Evidence that barley HMG-CoA reductase kinase is a member of the SNF1-related protein kinase family. Plant Physiol 112: 1141–1149

    Google Scholar 

  5. Carling D, Aguan K, Woods A, Verhoeven AJM, Beri RK, Brennan CH, Sidebottom C Davison MD and Scott J (1994) Mammalian AMP-activated protein kinase is homologous to yeast and plant protein kinases involved in the regulation of carbon metabolism. J Biol Chem 269: 11442–11448

    Google Scholar 

  6. Celenza JL and Carlson M (1986) A yeast gene that is essential for release from glucose repression encodes a protein kinase. Science 233: 1175–1180

    Google Scholar 

  7. Clarke PR and Hardie DG (1990) Regulation of HMG-CoA reductase: Identification of the site phosphorylated by the AMP-activated protein kinase in vitro and in intact rat liver. EMBO J 9: 2439–2446

    Google Scholar 

  8. Dale S, Wilson WA, Edelman AM and Hardie DG (1995) Similar substrate recognition motif for mammalian AMP-activated protein kinase, higher plant HMG-CoA reductase kinase-A, yeast SNF1 and mammalian calmodulin-dependant protein kinase I. FEBS Letts 361: 191–195

    Google Scholar 

  9. Davies SP, Carling D and Hardie DG (1989) Tissue distribution of the AMP-activated protein kinase, and lack of activation by cyclic AMP-dependent protein kinase, studies using a specific and sensitive peptide assay. Eur J Biochem 186: 123–128

    Google Scholar 

  10. Douglas P, Pigaglio E, Ferrer A, Halford NG and MacKintosh C (1997) Three spinach nitrate reductase/3-hydroxy-3-methylglutaryl-CoA reductase kinases that are regulated by reversible phosphorylation and/or Ca2+ ions. Biochem J 325: 101–109

    Google Scholar 

  11. Genetics Computer Group (1991) Program Manual for the GCG Package, Version 7, April 1991. 575 Science Drive, Wisconsin, USA 53711

  12. Giaquinta RT (1979) Sucrose translocation and storage in the sugar beet plant. Plant Physiol. 63: 828–832

    Google Scholar 

  13. Halford NG, Man AL, Barker JHA, Monger W, Shewry PR, Smith AM and Purcell PC (1994) Investigating the role of plant SNF1-related protein kinases. Biochem Soc Trans 22(4): 953–957

    Google Scholar 

  14. Halford NG, Vicente-Carbajosa J, Sabelli PA, Shewry PR, Hannappel U and Kreis M (1992) Molecular analyses of a barley multigene family homologous to the yeast protein kinase gene SNF1. Plant J 2: 791–797

    Google Scholar 

  15. Hannappel U, Vicente-Carbajosa J, Barker JHA, Shewry PR and Halford NG (1995) Differential expression of two barley SNF1-related protein kinase genes. Plant Molec Biol 27: 1235–1240

    Google Scholar 

  16. Hardie DG, Carling D and Halford N (1994) Roles of the Snf1/Rkin1/AMP-activated protein kinase family in the response to environmental and nutritional stress. Seminars in Cell Biol 5: 409–416

    Google Scholar 

  17. Hardy TA, Huang D and Roach PJ (1994) Interactions between cAMP-dependent and SNF1 protein kinases in the control of glycogen accumulation in Saccharomyces cerevisiae. J Biol Chem 269: 27907–27913

    Google Scholar 

  18. Harvey CW and Dutton JV (1993) Root quality and processing. In: Cooke DA and Scott RK (eds) The Sugar Beet Crop, pp 571–617. London, UK: Chapman and Hall

    Google Scholar 

  19. Johnson DA, Gautsch JW, Sportsman JR and Elder JH (1984) Improved technique utilising nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Analytical Technology 1: 3–8

    Google Scholar 

  20. Kimura M (1980) The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press

    Google Scholar 

  21. Leigh RA, Rees T, Fuller WA and Banfield J (1979) The location of acid invertase and sucrose in the vacuoles of storage roots of beet root (Beta vulgaris). Biochem J 174: 539–547

    Google Scholar 

  22. Le Guen L, Thomas M, Bianchi M, Halford NG and Kreis M (1992) Structure and expression of a gene from A. thaliana encoding a protein related to the SNF1 sub-family of protein kinases. Gene 120(2): 249–254

    Google Scholar 

  23. MacKintosh RW, Davies SP, Clarke PR, Weekes J, Gillespie JG, Gibb BJ and Hardie DG (1992) Evidence for a protein kinase cascade in higher plants: 3-hydroxy-3-methylglutaryl-CoA reductase kinase. Eur J Biochem 209: 923–931

    Google Scholar 

  24. Man AL, Purcell PC, Hannappel U and Halford NG (1997) Potato SNF1-related protein kinase: Molecular cloning, expression analysis and peptide kinase activity assay. Plant Molec Biol 34: 31–43

    Google Scholar 

  25. Morrell S and ap Rees T (1986) Sugar metabolism in developing tubers of Solanum tuberosum. Phytochem 25: 1579–1585

    Google Scholar 

  26. Muranaka T, Banno H and Machida Y (1994) Characterization of the tobacco protein kinase NPK5, a homologue of Saccharomyces cerevisiae SNF1 that constitutively activates expression of the glucose-repressible SUC2 gene for a secreted invertase of S. cerevisiae. Mol Cell Biol 14: 2958–2965

    Google Scholar 

  27. Sambrook J, Fritsch, EF and Maniatis T (1989) Molecular Cloning, A Laboratory Manual, 2nd Edition. New York: Cold Spring Harbor Laboratory Press

    Google Scholar 

  28. Sim ATR and Hardie DG (1988) The low activity of acetyl-CoA carboxylase in basal and glucagon-stimulated hepatocytes is due to phosphorylation by the AMP-activated protein kinase and not cyclic AMP-dependent protein kinase. FEBS Letts 233: 294–298

    Google Scholar 

  29. Sung S-JS, Xu D-P and Black CC (1989) Identification of actively filling sucrose sinks. Plant Physiol 89: 1117–1121

    Google Scholar 

  30. Thevelein JM (1994) Signal transduction in yeast. Yeast 10: 1753–1790

    Google Scholar 

  31. Thompson-Jaeger S, Francois J, Gaughran JP and Tatchell K (1991) Deletion of SNF1 affects the nutrient response of yeast and resembles mutations which activate the adenylate cyclase pathway. Genetics 129: 697–706

    Google Scholar 

  32. Woods A, Munday MR, Scott J, Yang X, Carlson M and Carling D (1994) Yeast SNF1 is functionally related to mammalian AMP-activated protein kinase and regulates acetyl-CoA carboxylase in vivo. J Biol Chem 269: 19509–19515

    Google Scholar 

  33. Zrenner R, Salanoubat M, Willmitzer L and Sonnewald U (1995) Evidence of the crucial role of sucrose synthase for sink strength using transgenic potato plants (Solanum tuberosum L.). Plant J 7: 97–107

    Google Scholar 

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Author notes

  1. Wendy A. Monger

    Present address: School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK

Authors and Affiliations

  1. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS18 9AF, UK

    Wendy A. Monger, Tudor H. Thomas, Patrick C. Purcell & Nigel G. Halford

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  1. Wendy A. Monger
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  2. Tudor H. Thomas
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  3. Patrick C. Purcell
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  4. Nigel G. Halford
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Monger, W.A., Thomas, T.H., Purcell, P.C. et al. Identification of a sucrose nonfermenting-1-related protein kinase in sugar beet (Beta vulgaris L.). Plant Growth Regulation 22, 181–188 (1997). https://doi.org/10.1023/A:1005845605696

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