Abstract
Fragaria ananassa (strawberry) callus, which produced high amounts of anthocyanin in the dark, was isolated from a cell line not producing anthocyanin. The isolated callus (FAR) was homogeneous and more than 90% of the cells were pigmented. The FAR callus accumulated more than 1000 μg of anthocyanin per g fresh cell in the dark. Four different basal solid media were examined to maintain FAR callus: Though growth rate and anthocyanin concentration were different on each media, total anthocyanin production was about the same at 400 μg anthocyanin/0.1 g fresh cell wt after 22 days. This FAR cell line could therefore be used for the industrial production of anthocyanin.
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Nakamura, M., Takeuchi, Y., Miyanaga, K. et al. High anthocyanin accumulation in the dark by strawberry (Fragaria ananassa) callus. Biotechnology Letters 21, 695–699 (1999). https://doi.org/10.1023/A:1005558325058
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DOI: https://doi.org/10.1023/A:1005558325058