Skip to main content
SpringerLink
Log in

Synthesis of disaccharide derivatives employing β-N-acetyl- d-hexosaminidase, β-d-galactosidase and β-d-glucuronidase

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

β-N-Acetyl-d-hexosaminidase from Aspergillus oryzae catalysed the stereo- and regiospecific formation of the 6-O-benzylated disaccharide derivatives GalNAcβ1-3(6- OBn)Galβ-SEt and GlcNAcβ1-3(6-OBn)Galβ-SEt, which were obtained in transglycosylation reactions employing ethyl 6- O-benzyl-1-thio-β-d-galactopyranoside as acceptor. Preparative amounts of the chitobiose derivative GlcNAcβ1- 3GlcNAcβ-OPhNO2-p was prepared as well. β- N-Acetyl-d-hexosaminidase from bovine testes catalysed the specific synthesis of GlcNAcβ1-3(6-OBn)GlcNH2β-SEt and GalNAcβ1-3(6-OBn)GlcNH2β-SEt, employing ethyl 2-amino-6-O-benzyl-2-deoxy-1-thio-β-d-glucopyranoside as acceptor. β-d-Glucuronidase from E. coli was found to catalyse the formation of GlcAβ1-3(6-OBn)GlcNH2β- SEt employing the same acceptor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Crout DHG, Sing S, Swoboda BEP, Critchley P, Gibson WT (1992) J. Chem. Soc. Chem. Commun.: 704–705.

  • Karlsson KA (1989) Annu. Rev. Biochem. 58: 309–350.

    Google Scholar 

  • Nilsson KGI (1987) Carbohydr. Res. 167: 95–103.

    Google Scholar 

  • Nilsson KGI (1988) Trends Biotechnol. 6: 256–264.

    Google Scholar 

  • Nilsson KGI (1989) Carbohydr. Res. 188: 9–17.

    Google Scholar 

  • Nilsson KGI (1990) Carbohydr. Res. 204: 79–83.

    Google Scholar 

  • Nilsson KGI, Fernandez-Mayoralas A (1991) Biotechnol. Lett. 13: 715–720.

    Google Scholar 

  • Nilsson KGI, Eliasson A, Lorek U (1995) Biotechnol. Lett. 17: 717–722.

    Google Scholar 

  • Nilsson KGI, Pan H, Larsson-Lorek U (1997) J. Carbohydr. Chem. 16: 459–477.

    Google Scholar 

  • Sing S, Packwood J, Crout DHG (1994) J. Chem. Soc. Chem. Commun.: 2227–2228.

  • Sing S, Packwood J, Samuel CJ, Critchley P, Crout DHG (1995) Carbohydr. Res. 279, 293–305.

    Google Scholar 

  • Thiem J, Sauerbrei B (1991) Angew. Chem. Int. Ed. Engl. 30: 1503–1505.

    Google Scholar 

  • Wong C-H (1993) Chimia 47: 63–78.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Glycorex AB, Sölvegatan 41, S-223 70, Lund, Sweden

    Kurt G.I. Nilsson, Anna Eliasson, Hefeng Pan & Mattias Rohman

Authors
  1. Kurt G.I. Nilsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anna Eliasson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hefeng Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mattias Rohman
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nilsson, K.G., Eliasson, A., Pan, H. et al. Synthesis of disaccharide derivatives employing β-N-acetyl- d-hexosaminidase, β-d-galactosidase and β-d-glucuronidase. Biotechnology Letters 21, 11–15 (1999). https://doi.org/10.1023/A:1005439302682

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005439302682

Search

Navigation