Skip to main content
SpringerLink
Log in

Effect of three anthocyaninless genes on germination in tomato (Lycopersicon esculentum Mill.) II. Seed germination under stress conditions

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Seeds from four pairs of tomato isogenic/near-isogenic lines (IL/NIL) differing for anthocyaninless of Hoffmann (ah), three pairs of IL/NILs differing for anthocyaninwithout (aw) and six pairs of IL/NILs differing for baby lea syndrome (bls) were evaluated for their germination ability under stress conditions: low and high temperature, salt and osmotic stress. Mutant genes affecting anthocyanin biosynthesis enhanced tomato germination capacity under all above mentioned treatments, except for the aw gene under a PEG-6000 treatment. Testa removal accelerated germination under stress conditions, but differences in time to 50% germination between the wild type lines and their ah-, aw- and bls-IL/NILs although diminished, were not eliminated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Atanassova, B., L. Shtereva & E. Molle, 1993. New potential for the improvement of tomato salt tolerance during germination. Proc. of the XIIth Eucarpia Meeting on Tomato Genetics and Breeding, Plovdiv (Bulgaria) 27–31 July: 81-87.

  • Atanassova, B., L. Shtereva & S. Daskalov, 1995. Effect of mutant genes on the germination abilities of tomato and pepper under optimal and stress conditions. Proc. of a Symp. ‘Induced Mutations and Molecular Techniques for Crop Improvement’ Vienna, 19–23 June 1995: 643-645.

  • Bongue-Bartelsman, M., D. O'Neill, Y. Tong & J.I. Yoder, 1994. Characterization of the gene encoding dihydroflavonol 4-reductase in tomato. Gene 138: 153-157.

    Article  PubMed  CAS  Google Scholar 

  • Bino, R.J., J.N. Vries, H.L. Kraak & J.G. Pijlen, 1992. Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination. Ann Bot 69: 231-236.

    Google Scholar 

  • de Castro, R.D., X. Zheng, Jan H.W. Bergervoet, C.H. Ric De Vos & R.J. Bino, 1995. β-Tubulin accumulation of DNA replication in imbibing tomato seeds. Plant Physiol 109: 499-504.

    PubMed  CAS  Google Scholar 

  • Dahal, P., R.A. Bradford & R.A. Jones, 1990. Effects of priming and endosperm integrity on seed germination rates of tomato genotypes. I. Germination at suboptimal temperature. J Expt Bot 41: 1431-1439.

    Google Scholar 

  • Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics, Vol 11: 1-21.

    Article  Google Scholar 

  • El Hassan, G.M., 1978. Preliminary evaluation of tomato cultivars for high temperature emergence. Acta Hort 84: 23-26.

    Google Scholar 

  • El Sayed, M.N. & C.A. John, 1973. Heritability studies of tomato emergence at different temperatures. J Am Soc Hort Sci 98: 440-443.

    Google Scholar 

  • Fooland, M.R. & R.A. Jones, 1991. Genetic analysis of salt tolerance during germination in Lycopersicon. Theor Appl Genet 81: 321-326.

    Article  Google Scholar 

  • Goldsbrough, A., F. Belzile & J.I. Yoder, 1994. Complementation of the tomato anthocyanin without (aw) mutant using the dehydroflavonol 4-reductase gene. Plant Physiol 105: 491-496.

    PubMed  CAS  Google Scholar 

  • Groot, S.P.C., B. Kieliszewska-Rokicka, E. Vermeer & C.M. Karssen, 1988. Gibberellin induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radicle protrusion. Planta 174: 500-504.

    Article  CAS  Google Scholar 

  • Hashimoto, T. & M. Tajima, 1980. Effects of ultraviolet irradiation on growth and pigmentation in seedlings. Plant Cell Physiol 21: 1559-1571.

    CAS  Google Scholar 

  • Johnson, N.L. & F.C. Leone, 1977. Statistics and experimental design, Vol II. Johns Wiley & Sons, New York-London-Sydney-Toronto, pp. 516.

    Google Scholar 

  • Jones, R.A., 1986. High salt tolerance potential in Lycopersicon species during germination. Euphytica 35: 575-582.

    Article  CAS  Google Scholar 

  • Karssen, C.M., S. Zagorski, J. Kepezynnski & S.P.C. Groot, 1989. Key role for endogenous gibberellins in the control of seed germination. Ann Bot 63: 71-80.

    CAS  Google Scholar 

  • Lakin, G.F., 1990. Biometrics. ‘Vishaia shkola’ Moscow, 350 pp. (in Russian).

  • Leviatov, Sh., O. Shoseyov & Sh. Wolf, 1994. Roles of different seed components in controlling tomato seed germination at low temperature. Scientia Horticulturae 56: 197-206.

    Article  Google Scholar 

  • Leviatov, Sh., O. Shoseyov & S. Wolf, 1995. Involvement of endomannanase in the control of tomato seed germination under low temperature conditions. Ann Bot 76: 1-6.

    Article  CAS  Google Scholar 

  • Li, J., T. Ou-Lee, R. Raba, R.G. Amundson & R.L. Last, 1993. Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation. Plant Cell 5: 171-179.

    Article  PubMed  CAS  Google Scholar 

  • Liptay, A. & P. Schopfer, 1983. Effect of water stress, seed coat restraint and abscisic acid upon different germination capabilities of two tomato lines at low temperature. Plant Physiol 73: 935-938.

    PubMed  CAS  Google Scholar 

  • Liptay, A. & N. Zariffa, 1993. Testing the morphological aspects of polyethylene glycol-primed tomato seeds with proportional odds analysis. Hort Sci 28(9): 881-883.

    Google Scholar 

  • Mancinelli, A.L., Z. Yaniv & P. Smith, 1967. Phytochrome and seed germination. I. Temperature dependence and relative Pfr levels in the germination of dark-germinating tomato seeds. Plant Physiol 42: 333-337.

    Article  PubMed  CAS  Google Scholar 

  • Maxon Smith, J.W. & D.B. Ritchie, 1982. A collection of near isogenic lines of tomato. Research tool of the future? Plant Mol Biol Newslett 3: 20-25.

    Google Scholar 

  • O'Neill, Sh.D., Y. Tong, B. Spörlein, G. Forkmann & J.I. Yoder, 1990. Molecular genetic analysis of chalcone synthase in Lycopersicon esculentum and an anthocyanin-deficient mutant. Mol Gen Genet 224: 279-288.

    Article  PubMed  Google Scholar 

  • Philouze, J., 1991. Description of isogenic lines, except for one, or two, monogenically controlled morphological traits in tomato, Lycopersicon esculentum, Mill. Euphytica 56: 121-131.

    Article  Google Scholar 

  • Rick, C.M. & R.T. Chetelat, 1993. Stocks lists. TGC Rep 43: 53-87.

    Google Scholar 

  • Scott, S.J. & R.A. Jones, 1982. Low temperature seed germination of Lycopersicon species evaluated by survival analysis. Euphytica 31: 869-883.

    Article  Google Scholar 

  • Scott, S.J. & R.A. Jones, 1985. Cold tolerance in tomato. I. Seed germination and early seedling growth of Lycopersicon esculentum. Physiol Plant 65: 487-492.

    Article  Google Scholar 

  • Scott, S.J. & R.A. Jones, 1986. Cold tolerance in tomato. II. Early seedling growth of Lycopersicon sp. Physiol Plant 66: 659-663.

    Article  Google Scholar 

  • Thompson, P.A., 1974. Characterization of the germination responses to temperature of vegetable seeds. I. Tomatoes. Sci Hort 2: 35-54.

    Article  Google Scholar 

  • Van Tunen, A.J., S.A. Hartman, L.A. Mur & J.N.M. Mol, 1989. Regulation of chalcone flavonone isomerase (CHI) gene expression in Petunia hybrida: the use of alternative promoters in corolla, anthers and pollen. Plant Mol Biol 12: 539-551.

    Article  CAS  Google Scholar 

  • Yoder, J.I., F. Belzile, Y. Tong & A. Goldsbrough, 1994. Visual markers for tomato derived from the anthocyanin biosynthetic pathway. Euphytica 79: 163-167.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Genetics ‘Akad. D. Kostoff’, Bulgarian Academy of Sciences, Sofia, 1113, Bulgaria

    Bistra Atanassova & Lydia Shtereva

  2. Faculty of Biology, University of Sofia, Bulgaria

    Emil Molle

Authors
  1. Bistra Atanassova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lydia Shtereva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Emil Molle
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Atanassova, B., Shtereva, L. & Molle, E. Effect of three anthocyaninless genes on germination in tomato (Lycopersicon esculentum Mill.) II. Seed germination under stress conditions. Euphytica 97, 31–38 (1997). https://doi.org/10.1023/A:1003005921080

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1003005921080

Search

Navigation