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The application of gene transfer techniques to marine resource management: recent advances, problems and future directions

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Abstract

Recent advantages in transgenic fish research are reviewed, with special reference to the methods for gene transfer. These include microinjection, electroporation, particle bombardment, and lipofection. The success and problems associated with each of these methods, and the possible applications of transgenic fish research to aquaculture are discussed.

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References

  • Arezzo, F., 1989. Sea urchin sperm as vector for foreign genetic transformation. Cell Biol. Int. Rep. 13: 391–404.

    CAS  Google Scholar 

  • Alam, J. & J. L. Cook, 1990. Reporter genes: application to the study of mammalian gene transcription. Analyt. Biochem. 188: 245–254.

    PubMed  CAS  Google Scholar 

  • Alestrom, P., G. Kisen, H. Klungland & O. Andersen, 1992. Fish gonadotropin-releasing hormone gene and molecular approaches for control of sexual maturation: development of a transgenic fish model. Mol. mar. Biol. Biotech. 1: 376–379.

    CAS  Google Scholar 

  • Bachere, E., E. Mialhe, D. Noel, V. Boulo, A. Morvan & J. Rodriguez, 1995. Knowledge and research prospects in marine mollusc and crustacean immunology. Aquaculture 132: 17–32.

    Google Scholar 

  • Bachiller, D., K. Schellander, J. Peli & U. Ruther, 1991. Liposome-mediated DNA uptake by sperm cells. Mol. Reprod. Dev. 30: 194–200.

    PubMed  CAS  Google Scholar 

  • Baranov, V. S., I. Gapala, P. Griyach, L. Kovach & K. Bodya, 1990. Incorporating macromolecules into the sex cells of male mice using electric impulse and DMSO. Tsitologiya I Genetika 24: 3–7.

    CAS  Google Scholar 

  • Behr, J. P., B. Demeneix, J. P. Loeffler & J. Perez-Mutul, 1989. Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA. Proc. natl. Acad. Sci. USA 86: 6982–6986.

    PubMed  CAS  Google Scholar 

  • Birnstiel, M. L. & M. Busslinger, 1989. Dangerous liaisons: spermatozoa as natural vectors for foreign DNA. Cell 57: 701–702.

    PubMed  CAS  Google Scholar 

  • Brem, G., B. Brenig, G. Horstgen-Schwark & E.-L. Winnacker, 1988. Gene transfer in Tilapia (Oreochromis niloticus). Aquaculture 68: 209–219.

    CAS  Google Scholar 

  • Brackett, B. G., W. Baranaska, W. Sawiicki & H. Koprosky, 1971. Uptake of heterologous genome by mammalian spermatozoa and its transfer to ova through fertilization. Proc. natl. Acad. Sci. USA 68: 353–357.

    PubMed  CAS  Google Scholar 

  • Buono, R. J. & P. J. Linser, 1992. Transient expression of RSVCAT in transgenic zebrafish made by electroporation. Mol. mar. Biol. Biotech. 1: 271–275.

    CAS  Google Scholar 

  • Camaioni, A., M. A. Russo, T. Odorisio, F. Gandolfi, V. M. Fazio & G. Siracusa, 1992. Uptake of exogenous DNA by mammalian spermatozoa: specific localization of DNA on sperm heads. J. Reprod. Fertil. 96: 203–212.

    Article  PubMed  CAS  Google Scholar 

  • Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward & D. C. Prasher, 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802–805.

    PubMed  CAS  Google Scholar 

  • Chen, T. T. & D. A. Powers, 1990. Transgenic fish. Trends Biotech. 8: 209–215.

    CAS  Google Scholar 

  • Chen, T. T., K. Kight, C. M. Lin, D. A. Powers, M. Hayat, N. Chatakondi, A. C. Ramboux, P. L. Duncan & R. A. Dunham, 1993. Expression and inheritance of RSVLTR-rtGH1 complementary DNA in the transgenic common carp, Cyprinus carpio. Mol. mar. Biol. Biotech. 2: 88–95.

    CAS  Google Scholar 

  • Chourrout, D., R. Guyomard, & L-M. Houdebine, 1986. High efficiency gene transfer in rainbow trout (Salmo gairdneriRich.) by microinjection into egg cytoplasm. Aquaculture 51: 143–150.

    CAS  Google Scholar 

  • Collas, P., H. Husebye & P. Alestrom, 1996. The nuclear localization sequence of the SV40 T antigen promotes transgene uptake and expression in zebrafish embryo nuclei. Transgenic Res. In press.

  • Culp, P., C. N-V. & N. Hopkins., 1991. High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs. Proc. natl. Acad. Sci. USA 88: 7953–7957.

    PubMed  CAS  Google Scholar 

  • Devlin, R. H., T. Y. Yesaki, E. M. Donaldson & C.L. Hew, 1995. Transmission and phenotypic effects of an antifreeze/GH gene construct in coho salmon (Oncorhynchus kisutch). Aquaculture 137: 161–169.

    CAS  Google Scholar 

  • Devlin, R. H., T. Y. Yesaki, E.M. Donaldson, S. J. Du & C.-L. Hew, 1993. Production of germline transgenic Pacific salmonids with dramatically increased growth performance. Can. J. Fish. aquat. Sci. 52: 1376–1384.

    Google Scholar 

  • Devlin, R. H., T. Y. Yesaki, C. A. Biagi, E. M. Donaldson, P. Swanson, W.-K. Chan, 1994. Extraordinary salmon growth. Nature 371: 209–210.

    Google Scholar 

  • Du, S. J., Z. Gong, G. L. Fletcher, M. A. Shears, M. J. King, D. R. Idler & C. L. Hew, 1992. Growth enhancement in transgenic Atlantic salmon by the use of an ‘all fish’ chimeric growth hormone gene construct. Biotechniques 10: 176–180.

    CAS  Google Scholar 

  • Dunham, R. A., J. Eash, J. Askins & T. M. Townes, 1987. Transfer of a metallothionein-human growth hormone fusion gene into channel catfish. Trans. am. Fish. Soc. 116: 87–91.

    CAS  Google Scholar 

  • Felgner, P. L., T. R. Gadek, M. Holm, R. Roman, H. W. Chan, M. Wenz, J. P. Northrop, G. M. Ringold & M. Danielsen, 1987. Lipofection: a highly efficient, lipid-mediated DNA transfection procedure. Proc. natl. Acad. Sci. USA 84: 7413–7417.

    PubMed  CAS  Google Scholar 

  • Fletcher, G. L. & P. L. Davies, 1991. Transgenic fish for aquaculture. Genet. Engineering 13: 331–70.

    CAS  Google Scholar 

  • Fletcher, G. L., M. A. Shears, M. J. King, D. L. Davies, & C. L. Hew, 1988. Evidence for antifreeze protein gene transfer in Atlantic salmon (Salmo salar). Can. J. Fish. aquat. Sci. 45: 352–357.

    Article  CAS  Google Scholar 

  • Francolini, M., M. Lavitrano, C. L. Lamia, D. French, L. Frati, F. Cotelli & C. Spadafora, 1993. Evidence for nuclear internalization of exogenous DNA into mammalian sperm cells. Mol. Reprod. Devel. 34: 133–139.

    PubMed  CAS  Google Scholar 

  • Gagne, M. B., F. Pothier & M. A. Sirard, 1991. Electroporation of bovine spermatozoa to carry foreign DNA in oocytes. Mol. Reprod. Devel. 29: 6–15.

    PubMed  CAS  Google Scholar 

  • Gavora, J. S., B. Benkel, H. Sasada, W. J. Cantwell, P. Fiser, R. M. Teacher, J. Nagal & M. P. Sabour, 1991. An attempt at sperm-mediated gene transfer in mice and chickens. Can. J. anim. Sci 71: 287–291.

    Article  Google Scholar 

  • Gendreau, S., V. Lardans, J. P. Cadoret & E. Mialhe, 1995. Transient expression of a luciferase reporter gene after ballistic introduction into Artemia franciscana(Crustacea) embryos. Aquaculture 133: 199–205.

    Google Scholar 

  • Gershon, H., R. Ghirlando, S. B. Guttman & A. Minsky, 1993. Model of formatin and structural features of DNA-cationic liposome complexes used for transfection. Biochemistry 32: 7143–7151.

    PubMed  CAS  Google Scholar 

  • Gibbs, P. D. L., A. Peek & G. Thorgaard, 1994. An in vivo screen for the luciferase transgene in zebrafish. Mol. mar. Biol. Biotech. 3: 307–316.

    CAS  Google Scholar 

  • Gong, Z., C. L. Hew & J. R. Vielkind, 1991. Functional analysis and temporal expression of promoter regions from fish antifreeze protein genes in transgenic Japanese medaka embryos. Mol. mar. Biol. Biotech. 1: 64–72.

    CAS  Google Scholar 

  • Gorman, C. M., L. Moffat & B. Howard, 1982. Recombinant genomes which express chloramphenicol acetyl transferase in mammalian cells. Mol. Cell. Biol. 2: 1044–1051.

    PubMed  CAS  Google Scholar 

  • Grasser, C. S. & R. T. Fraley, 1989. Genetically engineering plants for crop improvement. Science 244: 1293–1299.

    Google Scholar 

  • Gross, M. L., J. F. Schneider, N. Moav, B. Moav, C. Alvarez, S. H. Myster, Z. Liu, E.M. Hallerman, P. B. Hackett, K. S. Guise, A. J. Farae & A. R. Kapiscinski, 1992. Molecular analysis and growth evaluation of northern pike (Esox lucius) microinjected with growth hormone genes. Aquaculture 103: 253–273.

    CAS  Google Scholar 

  • Haseldoff, J. & B. Amos, 1995. GFP in plants. Trends Genet. 11: 328–329.

    Google Scholar 

  • Hayat, M., C. P. Joyce, T. M. Townes, T. T. Chen, D. A. Powers & R. A. Dunham. 1991. Survival and integration rate of channel catfish and common carp embryos microinjected with DNA at various developmental stages. Aquaculture 99: 249–255.

    Google Scholar 

  • Hervio, D., E. Bachere, N. Boulo, V. Cochennec, V. Vuillemin, Y. Le Coguic, G. Cailletaux, J. Mazurie & E. Mialhe, 1995. Establishment of an experimental infection protocol for the flat oyster, Ostrea edulis, with the intrahaemocytic protozoan parasite, Bonamia ostreae: application in the selection of parasiteresistant oysters. Aquaculture 132: 183–194.

    Google Scholar 

  • Hoci, S., T. Ninomiya, A. Mizuno, M. Honma & A. Yuchi, 1990. Fate of exogenous DNA carried into mouse eggs by spermatozoan. Anim. Biotech. 1: 25–30.

    Google Scholar 

  • Hodgkinson, S., 1995. GFP in Dictyostelium. Trends Genet. 11: 327–328.

    PubMed  CAS  Google Scholar 

  • Horan, R., R. Powell, S. McQuaid, F. Gannon & J. A. Houghton, 1991. Association of foreign DNA with porcine spermatozoa. Arch. Andro. 26: 83–92.

    CAS  Google Scholar 

  • Horan, R., R. Powell, J. M. Bird, F. Gannon & J. A. Houghton, 1992. Effects of electropermeabilization on the association of foreign DNA with pig sperm. Arch. Andro. 28: 105–114.

    CAS  Google Scholar 

  • Inoue, K., S. Yamashita, J-i. Hata, S. Kabeno, S. Sada, E. Nagahisa & T. Fujita, 1990. Electroporation as a new technique for producing transgenic fish. Cell Diff. Devel. 29: 123–128.

    CAS  Google Scholar 

  • Ivics, Z., Z. Izsvak & P. B. Hackett, 1993. Enhanced incorporation of transgenic DNA into zebrafish chromosomes by a retroviral integration protein. Mol. mar. Biol. Biotech. 2: 162–173.

    CAS  Google Scholar 

  • Kavumpurath, S., O. Anderson, G. Kisen & P. Alestrom, 1993. Gene transfer methods and luciferase gene expression in zebrafish, Brachydanio rerio(Hamilton). Israeli J. aquat. Bamidgeh 45: 154–163.

    Google Scholar 

  • Khoo, H.-W., L.-H. Ang, H.-B. Lim & K.-Y. Wong, 1992. Sperm cells as vector for introducing foreign DNA into zebrafish. Aquaculture 107: 1–9.

    CAS  Google Scholar 

  • Knight, D. E., 1981. Rendering cells permeable by exposure to electric fields. Tech. Cell Physiol. 113: 1–20.

    Google Scholar 

  • Knight, D. E. & M. C. Scrutton, 1986. Gaining access to the cytosol: the technique and some application of electropermeabilization. Biochem. J. 234: 497–506.

    PubMed  CAS  Google Scholar 

  • Lavitrano, M., A. Camaioni, V. M. Fazio, S. Dolci, M. G. Farace & S. Dolci, 1989. Sperm cells as vectors for introducing foreign DNA into eggs: genetic transformation of mice. Cell 57: 717–723.

    PubMed  CAS  Google Scholar 

  • Leemans, J., 1993. Ti to tomato, tomato to market: a decade of plant biotechnology. Biotechnology 11: 22–26.

    Google Scholar 

  • Lin, S., N. Gaiano, P. Gulp, J. C. Burns, T. Friedmann, J-K. Yee & N. Hopkins, 1994. Integration and germline transmission of a pseudotyped retroviral vector in zebrafish. Science 265: 666–669.

    PubMed  CAS  Google Scholar 

  • Lu, J-K., T. T. Chen, C. L. Chrisman, O. M. Andrisani & J. E. Dixon, 1992. Integration, expression, and germ-line transmission of foreign growth hormone genes in medaka (Oryzias latipes). Mol. mar. Biol. Biotech. 1: 366–375.

    CAS  Google Scholar 

  • Martinez, R., M. P. Estrada, J. Berlango, I. Guillen, O. Hernandez, E. Cabrera, R. Pimentel, R. Morales, F. Herrera, A. Morales, J. C. Pina, Z. Abad, V. Sanchez, P. Melamed, R. Lleonart & J. de la Fuente, 1996. Growth enhancement in trangenic tilapia by ectopic expression of tilapia growth hormone. Mol. mar. Biol. Biotech. 5: 62–70.

    CAS  Google Scholar 

  • McEvoy, T., M. Stack, B. Keane, B. T. Barry, J. Sreenan & F. Gannon. 1988. The expression of a foreign gene in salmon embryos. Aquaculture 68: 27–37.

    Google Scholar 

  • Moav, B., Z. Liu, L. D. Caldovic, M. L. Gross, A. J. Faras & P. B. Hackett, 1993. Regulation of expression of trangenes in developing fish. Transgenic Res. 2: 153–161.

    PubMed  CAS  Google Scholar 

  • Muller, F., Z. Ivics, F. Erdelyi, T. Papp, L. Varadi, L. Horvath, N. Maclean & L. Orban, 1992. Introducing foreign genes into fish eggs with electroporated sperm as carrier. Mol. mar. Biol. Biotech. 1: 276–281.

    CAS  Google Scholar 

  • Muller, F., Z. Ivics, L. Varadi, L. Menczel & L. Orban, 1993. Efficient transient expression system based on square pulse electroporation and in vivo luciferase assay of fertilized eggs. FEBS Lett 324: 27–32.

    PubMed  CAS  Google Scholar 

  • Nakanishi, A. & A. Iritani, 1993. Gene transfer in the chicken by sperm-mediated methods. Mol. Reprod. Dev. 36: 258–261.

    PubMed  CAS  Google Scholar 

  • Neumann, J. R., C. A. Morency & K. O. Russian, 1987. A novel rapid assay for chloramphenicol acetyltransferase gene expression. Biotechniques 5: 444–447.

    CAS  Google Scholar 

  • Nguyen, V. T., M. Morange & O. Bensaude, 1988. Firefly luciferase luminescence assays suing scintillation counters for quantitation in transfected mammalian ce;;s. Analyt. Biochem. 171: 404–408.

    PubMed  CAS  Google Scholar 

  • O’Hara, M. J., M. G. Ormerod, P. R. Imrie, J. H. Peacock & W. Asche, 1989. Electropermeabilization and electrosensitivity of different types of mammalian cells. In Neumann, E., A. E. Sowers & C. A. Jordan (eds), Electroporation and Electrofusion in Cell Biology. Plenum Press, New York: 319–341.

    Google Scholar 

  • Ozato, K., H. Kondoh, H. Inohara, T. Iwamatsu, Y. Wakamatsu amp; T. S. Okada. 1986. Production of transgenic fish: introduction and expression of chicken δ-crystallin gene in medaka embryos. Cell Diff. 19: 237–244.

    CAS  Google Scholar 

  • Palmiter, R. D., R. L. Brinster, R. E. Hammer, M. E. Trumbayer, M. G. Rosenfeld, N. C. Brinberg & R. M. Evans, 1982. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature 300: 611–615.

    PubMed  CAS  Google Scholar 

  • Patil, J. G. & H. K. Khoo, 1996. Nuclear internalization of foreign NDA by zebrafish spermatozoa and its enhancement by electroporation. J. exp. Zool. 274: 121–129

    PubMed  CAS  Google Scholar 

  • Penman, D. J., A. J. Beeching, S. Penn & N. Maclean, 1990. Factors affecting survival and integration following microinjection of novel DNA into rainbow trout eggs. Aquaculture 85: 35–50.

    CAS  Google Scholar 

  • Perez, A. R., R. Solana, R. Castro, R. Lleonart, R. De Armas, R. Martinez, A. Aguilar, L. Herrera & J. D. L. Fuente, 1991. Sperm cells mediated gene transfer in cattle. Biotech. Apl. 8: 90–94.

    Google Scholar 

  • Pines, J., 1995. GFP in mammalian cells. Trends Genet. 11: 326–327.

    PubMed  CAS  Google Scholar 

  • Powers, D.A., L. Hereford, T. Cole, C. Creech, T. T. Chen, C. M. Lin, K. Kight & R. Dunham, 1992. Electroporation: a method for transferring genes into the gametes of zebrafish (Brachydanio rerio), channel catfish (Ictaluruspunctatus), and common carp (Cyprinus carpio). Mol. mar. Biol. Biotech. 1: 301–308.

    CAS  Google Scholar 

  • Powers, D. A., V. L. Kirby, T. Cole & L. Hereford, 1995. Electroporation as an effective means of introducing DNA into abalone (Haliotis rufescens) embryos. Mol. mar. Biol. Biotech. 4: 369–376.

    CAS  Google Scholar 

  • Pursel, V. G. & C. E. Rexroad, Jr., 1993. Recent progress in the transgenic modification of swine and sheep. Mol. Reprod. Dev. 36: 251–254.

    PubMed  CAS  Google Scholar 

  • Pursel, V. G., C. A. Pinkert, K. F. Miller, D. J. Bolt, R. G. Campbell, R. D. Palmiter, R. L. Brinster & R. E. Hammer, 1989. Genetic engineering of livestock. Science 244: 1281–1288.

    PubMed  CAS  Google Scholar 

  • Rokkones, E., P. Alestrom, H. Skjervold & K. M. Gautvik, 1987. Microinjection and expression of a mouse metallothionein human growth hormone fusion gene in fertilized salmonid eggs. J. comp. Physiol. 158: 751–758.

    Google Scholar 

  • Rottmann, O. J., R. Antes, P. Hofer & G. Maierhofer, 1992. Liposome mediated gene transfer via spermatozoa into avian egg cells. J. anim. Breed. Genet. 109: 64–70.

    Google Scholar 

  • Sandford, J. C., 1988. The bioballistic process. Trends Biotech. 6: 299–302.

    Google Scholar 

  • Serpersu, E. H., K. Kinosita, Jr & T. Y. Tsong, 1985. Reversible and irreversible modification of erythrocytes by electrical breakdown. Biochim. Biophys. Acta 816: 332–348.

    Google Scholar 

  • Sheehy, R. E., M. Kramer & W. R. Hiatt, 1988. Reduction of polygalacturonase activity in tomato fruit by antisense RNA. Proc. natl. Acad. Sci. USA 85: 8805–8809.

    PubMed  CAS  Google Scholar 

  • Shears, M. A., G. L. Fletcher, C. L. Hew, S. Gauthier & P. L. Davis, 1991. Transfer, expression, and stable inheritance of antifreeze protein genes in atlantic salmon (Salmo salar). Mol. mar. Biol. Biotech. 1: 58–63.

    CAS  Google Scholar 

  • Shigekawa, K. & W. J. Dower, 1988. Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells. Biotechniques 6: 742–751.

    PubMed  CAS  Google Scholar 

  • Sin, F. Y. T., A. Bartley, S. Walker, S. Bulman, L. A. Allison, C. L. Hopkins & I. L. Sin, 1990. Electroporation of fish sperm for gene transfer. Cell Biol Int. Reports 14: 167.

    Google Scholar 

  • Sin, F. Y. T., A. L. Bartley, S. P. Walker, I. L. Sin, J. E. Symonds, L. Hawke & C. L. Hopkins, 1993. Gene transfer in chinook salmon (Oncorhynchus tshawytscha) by electroporating sperm in the presence of pRSV-lacZ DNA. Aquaculture 117: 57–69.

    CAS  Google Scholar 

  • Sin, F. Y. T., U. K. Mukherjee, J. C. McKenzie & I. L. Sin, 1995. Electroporation of abalone sperm enhances sperm-DNA association. J. Fish Biol. 47 (Suppl. A): 20–28.

    CAS  Google Scholar 

  • Smith, J. G., R. L. Walzem & J. B. German, 1993. Liposomes as agents of DNA transfer. Biochim. Biophys. Acta 1154: 327–340.

    PubMed  CAS  Google Scholar 

  • Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J.mol. Biol. 98: 503–517.

    PubMed  CAS  Google Scholar 

  • Sowers, A. E. & M. R. Lieber, 1986. Electropore diameters, lifetimes, numbers, and locations in individual erythrocyte ghosts. FEBS Letters 205: 179–184.

    PubMed  CAS  Google Scholar 

  • Stuart, G. W., J. V. McMurray & M. Westerfield, 1988. Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos. Development 103: 403–412.

    PubMed  CAS  Google Scholar 

  • Sukharev, S. I., V. A. Klenchin, L. V. Servo, L. V. Chernomordik & Y. A. Chizmadzhev, 1992. Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores. Biophys. J. 63: 1320–1327.

    Article  PubMed  CAS  Google Scholar 

  • Symonds, J. E., S. P. Walker & F. Y. T. Sin, 1994a. Electroporation of salmon sperm with plasmid DNA: evidence of enhanced sperm/DNA association. Aquaculture 119: 313–327.

    CAS  Google Scholar 

  • Symonds, J. E., S. P. Walker, F. Y. T. Sin & I. L. Sin, 1994b. Development of a mass gene transfer method in chinook salmon: optimization of gene transfer by electroporated sperm. Mol. mar. Biol. Biotech. 3: 104–111.

    CAS  Google Scholar 

  • Szelei, J., L. Varadi, F. Muller, F. Erdelyi, L. Orban, L. Horvath & E. Dudo. 1994. Liposome-mediated gene transfer in fish embryos. Transgenic Res. 3: 116–119.

    CAS  Google Scholar 

  • Takagi, S., T. Sasado, G. Tamiya, K. Ozato, Y. Wakamatsu, A. Takeshita & M. Kimura, 1994. An efficient expression vector for transgenic medaka construction. Mol. mar. Biol. Biotech. 3: 192–199.

    CAS  Google Scholar 

  • Tamiya, E., T. Sugiyama, K. Masaki, A. Hirose, T. Okoshi & I. Karube, 1990. Spatial imaging of luciferase gene expression in transgenic fish. Nucleic Acids Res. 18: 1072.

    PubMed  CAS  Google Scholar 

  • Tewari, R., C. Michard-Vanhee, E. Parrot & D. Chourrout, 1992. Mendelian transmission, structure and expression of transgenes following their injection into the cytoplasm of trout eggs. Transgenic Res. 1: 250–260.

    CAS  Google Scholar 

  • Tsai, H-J. & F. S. Tseng, 1994. Electroporation of a foreign gene into black Acanthopagrus schlegeliembryos. Fish. Sci. 60: 787–788.

    CAS  Google Scholar 

  • Tsai, H. J., F. S. Tseng & I. C. Liao, 1995. Electroporation of sperm to introduce foreign DNA into the genome of loach (Misgurnus anguillicaudatus). Can. J. Fish. aquat. Sci. 52: 776–787.

    Article  CAS  Google Scholar 

  • Tsong, T. Y., 1983. Voltage modulation of membrane permeability and energy utilization in cells. Bioscience Rep. 3: 487–505.

    CAS  Google Scholar 

  • Ueno, K., S. Hamaguchi, K. Ozato, J-h. Kang & K. Inoue, 1994. Foreign gene transfer into nigorobuna (Carassius auratus grandoculis). Mol. mar. Biol. Biotech. 3: 235–242.

    CAS  Google Scholar 

  • Weaver, J. C., 1993. Electroporation: a general phenomenon for manipulating cells and tissues. J. Cell Biochem. 51: 426–435.

    PubMed  CAS  Google Scholar 

  • Winkler, C., J. R. Vielkind & M. Schartl, 1991. Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes). Mol. Gen. Genet. 226: 129–140.

    PubMed  CAS  Google Scholar 

  • Xie, Y., D. Liu, J. Zou, G. Li & Z. Zhu, 1989. Novel gene transfer in the fertilized eggs of loach via electroporation. Acta Hydrobiol. Sin. 13: 387–389.

    Google Scholar 

  • Xie, Y., L. Dong, J. Zou, G. Li & Z. Zhu, 1993. Gene transfer via electroporation in fish. Aquaculture 111: 207–213.

    CAS  Google Scholar 

  • Yamada, E., M. Matsuoka & F. Yamazaki, 1988. Introduction of exotic reagents into denuded eggs of goldfish and crucian carp by electroporation. Nippon Suisan Gakkaishi 54: 2043.

    Google Scholar 

  • Yoon, S. J., E. M. Hallerman, M. L. Gross, Z. Liu, J. F. Schneider, A. J. Fareas, P. B. Hackett, A. R. Kapuscinski & K. S. Guise, 1990. Transfer of the gene for neomycin resistance into goldfish, Carassius auratus. Aquaculture 85: 21–33.

    CAS  Google Scholar 

  • Yu, J. K., W. Yan, Y. L. Zhang, Y. Shen & S. Y. Yan, 1994. Spermmediated gene transfer andmethod of detection of integrated gene by PCR. Acta Zool. Sin. 40: 96–99.

    CAS  Google Scholar 

  • Yuko, W., H. Hashimoto, M. Kinoshita, T. Iwamatsu, Y. Hyodo-Taguchi, H. Tomita, M. Sakaguchi & K. Ozato, 1993. Generation of germ-line chimerase in medaka (Oryzias latipes) Mol. mar. Biol. Biotech. 2: 325–332.

    Google Scholar 

  • Zelenin, A. V., A. A. Alimov, V. A. Barmintez, A. O. Beniumov, I. A. Zelenina, A. M. Krasnov & V. A. Kolesnikov, 1991. The delivery of foreign genes into fertilised fish eggs using high-velocity microprojectiles. FEBS Lett. 287: 118–120.

    PubMed  CAS  Google Scholar 

  • Zhao, X., P. J. Zhang & T. K. Wong, 1993. Application of baekonization: a new approach to produce transgenic fish. Mol. mar. Biol. Biotech. 2: 63–69.

    Google Scholar 

  • Zhang, P., M. Hayat, C. Joyce, L. I. Gonzalez-Villasenor, C.M. Lin, R. A. Dunham, T. T. Chen & D. A. Powers, 1990. Gene transfer, expression and inheritance of pRSV-rainbow trout-GH in the common carp, Cyprinus carpio(Linnaeus). Mol. Reprod. Dev. 25: 3–13.

    PubMed  CAS  Google Scholar 

  • Zhu, Z., G. Li, L. He & S. Chen, 1985. Novel gene transfer into the fertilization eggs of gold fish (Carassius auratusL. 1758). Z. angew. Ichthyol. 1: 31–34.

    CAS  Google Scholar 

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Sin, F.Y.T., Mukherjee, U.K., Walker, L. et al. The application of gene transfer techniques to marine resource management: recent advances, problems and future directions. Hydrobiologia 352, 263–278 (1997). https://doi.org/10.1023/A:1003000127867

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  • DOI: https://doi.org/10.1023/A:1003000127867

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