biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 42:197-208, 1999 | DOI: 10.1023/A:1002152416883

Restriction Fragment Length Polymorphism and Random Amplified Polymorphic DNA Analysis of Chickpea Accessions

H. Banerjee1, R.A. Pai1, R.P. Sharma1
1 IARI, NRC on Plant Biotechnology, New Delhi, India

Genetic diversity analysis was carried out in chickpea accessions using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) techniques. RFLP analysis using 26 Pst I sub-genomic clones on ten chickpea accessions in 130 probe-enzyme combinations detected polymorphism with only two clones. Pst I clones, CG 141 detected polymorphism in ICC 4918 and Pusa 209 while CG 500 detected polymorphism in Pusa 261, ILC 26 and in ILC 13326. These clones detected very few polymorphic markers. Analysis using 10 Eco RI clones on twelve chickpea accessions have shown better hybridisation signal and one clone detected polymorphism in Pusa 256. RFLP analysis of both cultivated and wild Cicer species using heterologous DNA probe Cab3C revealed polymorphism only in wild Cicer species (Cicer reticulatum L., JM 2100). RAPD analysis of 13 chickpea accessions which includes mutants of C 235 and E100Y showed greater degree of polymorphism with 1 - 5 unique DNA bands for all the accessions. Phylogenetic analysis of the RAPD data helped to group the accessions. C 235 and its mutants were found to be closely grouped while E100Y and its mutant E100Ym grouped apart. Desi and kabuli chickpea accessions however, could not be separately grouped.

Keywords: Cicer arietinum; Cicer reticulatum; DNA polymorphism; genetic diversity; phylogeny
Subjects: chickpea accessions, genetic diversity analysis; Cicer arietinum; Cicer reticulatum; DNA polymorphism, chickpea accessions; genetic diversity, chickpea accessions; phylogeny, chickpea accessions; random amplified polymorphic DNA, RAPD markers, accessions, genetic diversity; restriction fragment length polymorphism, RFLP, accessions, genetic diversity

Published: September 1, 1999  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Banerjee, H., Pai, R.A., & Sharma, R.P. (1999). Restriction Fragment Length Polymorphism and Random Amplified Polymorphic DNA Analysis of Chickpea Accessions. Biologia plantarum, 42(2), 197-208. doi: 10.1023/A:1002152416883
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Ahmed, F., Gaur, P.M., Slinkard, A.E.: Isozyme polymorphism and phylogenetic interpretations in genus Cicer L.-Theor. appl. Genet. 83: 620-627, 1992. Go to original source...
    2. Birnboim, H.C., Doly, J.: A rapid alkaline extraction procedure for screening recombinant plasmid DNA.-Nucl. Acids Res. 7: 1413-1423, 1979. Go to original source...
    3. Davis, L.G., Dibner, M.D., Battey, J.F.: Basic Methods in Molecular Biology.-Elsevier Science Publishing Co., New York 1986. Go to original source...
    4. Devos, K.M., Gale, M.D.: The use of random amplified polymorphic DNA markers in wheat.-Theor. appl. Genet. 84: 567-572, 1992. Go to original source...
    5. Doyle, J.J., Doyle, J.L.: A rapid isolation procedure for small quantities of fresh leaf tissue.-Phytochem. Bull. 19: 11-15, 1987.
    6. Foolad, M.R., Jones, R.A., Rodriguez, R.L.: RAPD markers for constructing intraspecific tomato genetic maps.-Plant Cell Rep. 12: 293-297, 1993. Go to original source...
    7. Gaur, P.M., Slinkard, A.E.: Genetic control and linkage relations of additional isozyme markers in chickpea.-Theor. appl. Genet. 80: 648-656, 1990a. Go to original source...
    8. Gaur, P.M., Slinkard, A.E.: Inheritance and linkage of isozyme coding genes in chickpea.-J. Hered. 81: 455-461, 1990b. Go to original source...
    9. Gaur, P.M., Slinkard, A.E.: Duplication of the structural gene for the plastid specific isozyme of aldolase in Cicer.-Genome 34: 151-155, 1991. Go to original source...
    10. Gowen, S.R., Orton, M., Thurley, B., White, A.: Variation in pathogenecity of Ascochyta rabei on chickpeas.-Trop. Pest Manage. 35: 180-186, 1989. Go to original source...
    11. Helentjaris, T., King, G., Slocum, M., Siedenstrang, C., Wegman, S.: Restriction fragment length polymorphisms as probes for plant diversity and their development as tools for applied plant breeding.-Plant mol. Biol. 5: 109-118, 1985. Go to original source...
    12. Kazan, K., Muehlbauer, F.J.: Allozyme variation and phylogeny in annual species of Cicer (Leguminoseae).-Plant Syst. Evol. 175: 11-21, 1991. Go to original source...
    13. Kazan, K., Muehlabuer, F.J., Weeden, N.W., Ladizinsky, G.: Inheritance and linkage relationships of morphological and isozyme loci in chickpea (Cicer arietinum L.).-Theor. appl. Genet. 86: 417-426, 1993. Go to original source...
    14. Ladizinsky, G., Adler, A.: The origin of chickpea (Cicer arietinum L.).-Euphytica 25: 211-217, 1976. Go to original source...
    15. Miller, J.C., Tanksley, S.D.: Effect of different restriction enzymes, probe source, and probe length on determining restriction fragment length polymorphism in tomato.-Theor. appl. Genet. 80: 385-389, 1990. Go to original source...
    16. Moreno, M.T., Cubero, J.I.: Variation in Cicer arietinum L.-Euphytica 27: 465-485, 1979. Go to original source...
    17. Muehlbauer, F.J., Singh, K.B.: Genetics of chickpea.-In: Saxena, M.C., Singh, K.B. (ed.): The Chickpea. Pp. 99-126. CAB Int. Publication, UK 1987.
    18. Nei, M., Li, W.H.: Mathematical model for studying genetic variation in terms of restriction endonuclease.-Proc. nat. Acad. Sci. USA 76: 5269-5273, 1979. Go to original source...
    19. Nodari, R.O., Koinage, E.M.K., Kelley, J.D., Gepts, P.: Towards an integrated linkage map of common bean. 1. Development of genomic probes and levels of restriction fragment length polymorphism.-Theor. appl. Genet. 84: 186-192, 1992. Go to original source...
    20. Rohlf, F.J.: NTSYS-PC, Numerical Taxonomy and Multivariate Analysis System.-Applied Biostatistics Inc., New York 1993.
    21. Saitou, N., Nei, M.: The neighbour-joining method: a new method for reconstructing phylogenetic trees.-Mol. Biol. Evol. 4: 406-425, 1987.
    22. Sharma, P.C., Winter, A., Bunger, T., Huttel, B., Weigand, F., Weising, K., Kahl, G.: Abundance of polymorphism of di-, tri-, and tetranucleotide repeats in chickpea (Cicer arietinum L.).-Theor. appl. Genet. 90: 90-96, 1995. Go to original source...
    23. Tanksley, S.D., Young, N.D., Paterson, A.: RFLP mapping in plant breeding: new tools for an old science.-Bio/Technology 7: 257-264, 1989. Go to original source...
    24. Tayyar, R.I., Waines, J.G.: Genetic relationships among annual species of Cicer (Fabaceae) using isozyme variation.-Theor. appl. Genet. 92: 245-254, 1996. Go to original source...
    25. Udupa, S.M., Sharma, A., Sharma, R.P., Pai, R.A.: Narrow genetic variability in Cicer arietinum L. as revealed by RFLP analysis.-J. Plant Biochem. Biotech. 2: 84-86, 1993. Go to original source...
    26. Van Rheenen, H.A.: Biotechnology and chickpea breeding.-Int. Chickpea Newslett. 26: 14-17, 1992.
    27. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.-Nucl. Acids Res. 18: 6531-6535, 1990. Go to original source...
    28. Winter, P., Kahl, G.: Molecular technologies for plant improvement.-World J. Microbiol. Biotechnol. 11: 438-448, 1995. Go to original source...

    Return to the content