biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 41:523-532, 1998 | DOI: 10.1023/A:1001836215254

Immunogold localization of trans-zeatin riboside in embryo and endosperm during early fruit drop of Malus domestica

M. Ruffini Castiglione1
1 Dipartimento di Scienze Botaniche, Università di Pisa, Via L. Ghini 5, Pisa, Italy

The specificity of a monoclonal antibody IgG1, raised against trans-zeatin riboside-keyhole limpet hemocyanin conjugate, was investigated by means of inhibition experiments with soluble competing antigens. A competitive enzyme immunoassay was set up, with immobilized antigen. The analysis of the cross reaction profile enabled a study of the specificity of the antigen-antibody interaction. The antibody was able to distinguish the trans form of zeatin riboside from the cis form (cross reaction index = 1 %); cross reactions with ribose, adenine, adenosine and other related heterologous antigens were not detectable over the range of concetration tested. The recognition centres for the antibody seem to be the purine ring and the R substituent, especially in its hydroxymethyl group. Employment of this monoclonal antibody to localize cytokinins in control and shedding affected fruits of Malus domestica Borkh. evidenced high content of trans-zeatin riboside in developing seeds, differences in its content in embryo and endosperm, and a strong reduction of its content in the tissues of drop fruits. This decrease may be an important component responsible for early fruit abscission.

Keywords: cytokinins; fruit abscission; immunohistochemistry; monoclonal antibodies; seed development
Subjects: apple tree, early fruit drop; cytokinins, early fruit drop; fruit abscission, early; immunogold localization of trans-zeatin riboside; Malus domestica; monoclonal antibodies; seed development; trans-zeatin riboside, embryo, endosperm

Published: May 1, 1998  Show citation

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Ruffini Castiglione, M. (1998). Immunogold localization of trans-zeatin riboside in embryo and endosperm during early fruit drop of Malus domestica. Biologia plantarum, 41(4), 523-532. doi: 10.1023/A:1001836215254
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