biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 41:145-148, 1998 | DOI: 10.1023/A:1001793306811

Photoautotrophic in vitro Multiplication of the Orchid Dendrobium under CO2 Enrichment

A. Mitra, S. Dey, S.K. Sawarkar

An attempt to reduce the production cost on tissue cultured plants, photoautotrophic culture of a high value orchid Dendrobium was established under CO2-enriched conditions. The shoot length and the number of leaves were almost equal in plantlets grown on medium with 2 % sucrose or without sucrose and under normal or enhanced (40 g m-3) CO2 concentration, whereas the fresh and dry masses were higher in cultures grown in sucrose containing media or under CO2 enrichment. Development of roots was observed only on media without sucrose, but CO2 enrichment did not have significant effects on in vitro rootings.

Keywords: growth parameters; micropropagation; sugar-free medium
Subjects: Dendrobium sp.; in vitro culture, orchid multiplication, sugar-free medium

Published: January 1, 1998  Show citation

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Mitra, A., Dey, S., & Sawarkar, S.K. (1998). Photoautotrophic in vitro Multiplication of the Orchid Dendrobium under CO2 Enrichment. Biologia plantarum, 41(1), 145-148. doi: 10.1023/A:1001793306811
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    References

    1. Buddendorf-Joosten, J.M.C., Woltering, E.J.: Components of the gaseous environment and their effects on plant growth and development in vitro.-Plant Growth Regul. 15: 1-16, 1994. Go to original source...
    2. Deng, R., Donnelly, D.J.: In vitrohardening of red raspberry by CO2enrichment and reduced medium sucrose concentration.-HortScience 28: 1048-1051, 1993. Go to original source...
    3. Jeong, B.R., Fujiwara, K., Kozai, T.: Environmental control and photoautotrophic micropropagation.-Hort. Rev. 17: 125-172, 1995. Go to original source...
    4. Kirdmance, C., Kubota, C., Jeong, B.R., Kozai, T.: Photoautotrophic multiplication of Cymbidiumprotocorm-like bodies.-Acta Hort. 319: 243-248, 1992. Go to original source...
    5. Kozai, T., Kubota, C., Watanabe, I.: Effects of basal medium composition on the growth of carnation plantlets in auto-and mixotrophic tissue culture.-Acta Hort. 230: 159-166, 1988. Go to original source...
    6. Kozai, T.: Photoautotrophic micropropagation.-In VitroCell Dev. Biol. 271: 47-51, 1991. Go to original source...
    7. Mitra, A.: Photoautotrophic Micropropagation of Industrially Important Ornamentals.-Master of Technology.-Thesis. Indian Institute of Technology, Kharagpur 1994.
    8. Mitra, A., Khan, B.M., Rawal, S.K.: Photoautotrophic shoot culture: an economical alternative for the production of total alkaloid in Catharanthus roseus(L.) G. Don.-Curr. Sci. 73: 608-609, 1997.
    9. Pospíšilová, J., Čatský, J., Šesták, Z.: Photosynthesis in plants cultivated in vitro.-In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 525-540. Marcel Dekker, New York-Basel-Hong Kong 1997.
    10. Sharrna, N.K.: Automation in plant tissue culture.-Curr. Sci. 62: 507-512, 1992.
    11. Solárová, J., Pospíšilová, J.: Effect of carbon dioxide enrichment during in vitrocultivation and acclimation to ex vitroconditions.-Biol. Plant. 39: 23-30, 1997. Go to original source...
    12. Solárová, J., Pospíšilová, J., Čatský, J., Šantrůček, J.: Photosynthesis and growth of tobacco plantlets in dependence of carbon supply.-Photosynthetica 23: 629-637, 1989.
    13. Tichá, I.: Optimization of photoautotrophic tobacco in vitroculture: effect of suncaps closures on plantlet growth.-Photosynthetica 32: 475-479, 1996.
    14. Vacin, E.F., Went, F.W.: Some pH changes in nutrient solutions.-Bot. Gaz. 110: 605-613, 1949. Go to original source...

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