biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:349-355, 2003 | DOI: 10.1023/A:1024313832737

Rapid Micropropagation of Five Cultivars of Mulberry

B.S. Bhau1, A.K. Wakhlu1
1 Plant Tissue Culture Laboratory, Department of Botany, University of Jammu, Jammu, India

Multiple shoots were initiated from nodal and shoot tip explants collected from mature trees of Morus alba L. cultivars Chinese White, Kokuso-27 and Ichinose, and M. multicaulis Perr. cultivars Goshoerami and Rokokuyaso after 2 weeks of culture. Nodal explants were more responsive than shoot tip explants. Murashige and Skoog basal medium was found to be most suitable medium and 6-benzylaminopurine was the most effective cytokinin for shoot induction. Explants collected between April and September evoked better response than the explants collected between October and March. Shoots were multiplied by transferring nodal explants excised from in vitro raised shoots onto a medium containing cytokinins. Sucrose was the most suitable carbon source examined for shoot multiplication. An increase in shoot multiplication rate was noticed upto 4 - 5 subcultures. Nodal explants rooted on an auxin-supplemented medium. The acclimatized plants were successfully transplanted in the field.

Keywords: axillary shoot proliferation; carbon source; Morus alba; Morus multicaulis; subculture
Subjects: auxins; axillary shoot proliferation; cultivar differences; cytokinins; in vitro culture, carbon source; in vitro culture, micropropagation; in vitro culture, nutrient media modification; Morus alba; Morus multicaulis; mulberry, micropropagation

Prepublished online: April 1, 2003; Published: November 1, 2003  Show citation

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Bhau, B.S., & Wakhlu, A.K. (2003). Rapid Micropropagation of Five Cultivars of Mulberry. Biologia plantarum, 46(3), 349-355. doi: 10.1023/A:1024313832737
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