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Fertile somatic hybrids of Solanum etuberosum (+) dihaploid Solanum tuberosum and their backcrossing progenies: relationships of genome dosage with tuber development and resistance to potato virus Y

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Abstract

The wild non-tuberous species Solanumetuberosum is resistant to biotic andabiotic stresses, but is very difficult tocross with cultivated potato. Therefore,interspecific somatic hybrids between adihaploid clone of potato S.tuberosum (2n=2x=24, AA genome) and thediploid species S. etuberosum(2n=2x=24, EE genome) were produced byprotoplast fusion. Among the 7 fertilefusion hybrids analysed by genomic insitu hybridisation (GISH), three groups ofplants were found with the genomicconstitution of AAEE, AAEEEE and AAAAEE.Four fusion hybrids had exactly theexpected chromosome composition, while eachof the three aneuploid hybrids had lost twochromosomes of S. etuberosum. Twobackcross progenies were developed, andGISH analysis was applied to analysetransmission of the parental chromosomesinto the sexual generations. BC1hybrids derived from the crosses of thehexaploid somatic hybrids with tetraploidpotato were pentaploid with thetheoretically expected genomic compositionor with slight deviation from thisexpectation. In the three BC2 hybridsanalysed by GISH seven to 12 chromosomes ofS. etuberosum were detected in thepredominant S. tuberosum background.No recombinant chromosomes in the hybridswere detected. Genome dosage affects tuberformation in hybrids and their progenies,but has less effect on resistance to potatovirus Y (PVY) in fusion hybrids. Severalgenotypes of the fusion hybrids andBC1 progeny did not show viralinfection even in the graftingexperiments.

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References

  • Chavez, R., C.R. Brown & M. Iwanaga, 1988. Application of interspecific sesquiploidy to introgression of PLRV resistance from non-tuberbearing Solanum etuberosum to cultivated potato germplasm. Theor Appl Genet 76: 497-500.

    Google Scholar 

  • Chen, Y.-K.H., J.P. Palta, J.B. Bamberg, H. Kim, T. Geraldin, G.T. Haberlach & J.P. Helgeson, 1999. Expression of nonacclimated freezing tolerance and cold acclimation capacity in somatic hybrids between hardy wild Solanum species and cultivated potato. Euphytica 107: 1-8.

    Google Scholar 

  • Dong, F., R.G. Novy, J.P. Helgeson & J. Jiang, 1999. Cytological characterization of potato-Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization. Genome 42: 987-992.

    Google Scholar 

  • Gavrilenko, T., R. Thieme & H. Tiemann, 1999. Assessment of genetic and phenotypic variation in intraspecific potato somatic hybrids. Plant Breeding 118: 205-213.

    Google Scholar 

  • Gavrilenko, T., J. Larkka, E. Pehu & V.-M. Rokka, 2002. Identification of mitotic chromosomes of tuberous and non-tuberous Solanum species (Solanum tuberosum and Solanum brevidens) by GISH (genomic in situ hybridization) in their interspecific hybrids. Genome 45: 442-449.

    Google Scholar 

  • Johnston, S.A. & R.E. Hanneman, 1982. Manipulation of Endospermal Balance Number overcome crossing barriers between diploid Solanum species. Science (Washington D.C.) 217: 446-448.

    Google Scholar 

  • McGrath, J.M., & J.P. Helgeson, 1998. Differential behaviour of Solanum brevidens ribosomal DNA loci in a somatic hybrid and its progeny with potato. Genome 41: 435-439.

    Google Scholar 

  • McGrath, J.M., C.E. Williams, G.T. Haberlach, S.M. Wielgus, T.F. Uchytil & J.P. Helgeson, 2002. Introgression and stabilization of Erwinia tuber soft rot resistance into potato after somatic hybridisation of Solanum tuberosum and S. brevidens. Amer J of potato Res 79: 19-24.

    Google Scholar 

  • Naess, S.K., J.M. Bradeen, S.M. Wielgus, G.T. Haberlach, J.M.McGrath & J.P. Helgeson, 2000. Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet 101: 697-704.

    Google Scholar 

  • Novy, R. & J.P. Helgeson, 1994a. Somatic hybrids between Solanum tuberosum and diploid, tuber-bearing Solanum clones. Theor Appl Genet 89: 775-782.

    Google Scholar 

  • Novy, R. & J.P. Helgeson, 1994b. Resistance to potato virus Y in somatic hybrids between Solanum etuberosum and Solanum tuberosum × S. berthaultii hybrid. Theor Appl Genet 89: 783-786.

    Google Scholar 

  • Novy, R., A. Nasruddin, D.W. Ragsdale & E.B. Radcliffe, 2002. Genetic resistances to potato leafroll virus, potato virus Y, and green peach aphid in progeny of Solanum etuberosum. Amer J of Potato Res 79: 9-18.

    Google Scholar 

  • Perez, F., A. Menendez, P. Dehal & C.F. Quiros, 1999. Genomic structural differentiation in Solanum: comparative mapping of the A-and E-genomes. Theor Appl Genet 98: 1183-1193.

    Google Scholar 

  • Ramanna, M. & J. Hermsen, 1981. Structural hybridity in the series Etuberosa of the genus Solanum and its bearing on crossability. Euphytica 30: 15-31.

    Google Scholar 

  • Ramanna, M. & M. Wagenvoort, 1976. Identification of the trisomic series in diploid Solanum tuberosum L., group tuberosum I. Chromosome identification. Euphytica 30: 15-31.

    Google Scholar 

  • Schwarzacher, T. & J.S. Heslop-Harrison, 1994. Direct fluorochrome labeled DNA probes for direct fluorescent in situ hybridization to chromosomes. In: P.G. Isaac (Ed.), Methods in Molecular Biology. Humana Press Inc Totowa NJ 28: 8-17.

    Google Scholar 

  • Thieme, R., T. Gavrilenko, T. Thieme & U. Heimbach, 1999. Production of potato genotypes with resistance to Potato Virus Y by biotechnological methods. In: A. Altmann et al. (Eds.), Plant Biotechnology and In Vitro Biology in the 21st Century, pp. 557-560. Kluwer Academic Publishers.

  • Thieme, T. & R. Thieme, 1998. Evaluation of resistance to potato virus Y (PVY) in wild species and potato breeding clones of the genus Solanum. Aspects Appl Biol 52: 355-359.

    Google Scholar 

  • Valkonen, J.P.T., G. Brigneti, L.F. Salazar, E. Pehu & R.W. Gibson, 1992a. Interactions of the Solanum spp. of the Etuberosa group and nine potato-infecting viruses and viroid. Ann Appl Biol 120: 301-313.

    Google Scholar 

  • Valkonen, J.P.T., G. Brigneti & E. Pehu, 1992b. Resistance to Myzus persicae (Suls.) in wild potatoes of the series Etuberosa. Acta Agric Scand, Sectt B, Soil and Plant Sci 42: 118-127.

    Google Scholar 

  • Valkonen, J.P.T., Y.-S. Xu, V.-M. Rokka, S. Pulli & E. Pehu, 1994. Transfer of resistance to potato leafroll virus, potato virus Y and potato virus X from Solanum brevidens to S. tuberosum through symmetric and designed asymmetric somatic hybridisation. Ann Appl Biol 124: 351-362.

    Google Scholar 

  • Watanabe, K., M. Orrillo, S. Vega, J. Valkonen, E. Pehu, A. Hurtado & S. Tanksley, 1995. Overcoming crossing barriers between nontuber-bearing and tuber-bearing Solanum species: towards potato germplasm enhancement with a broad spectrum of solanaceous genetic resources. Genome 38: 27-35.

    Google Scholar 

  • Wolters, A.M.A., H.C.H. Schoenmakers, S. Kamstra, J. van Eden, M. Koornneef & J.H. de Jong, 1994. Mitotic and meiotic irregularities in somatic hybrids of Lycopersicon esculentum and Solanum tuberosum. Genome 37: 726-735.

    Google Scholar 

  • Zhong, X.-B., J.H. de Jong & P. Zabel, 1996. Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescence in situ hybridization (FISH). Chromos Res 4: 24-28.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Biotechnology, N.I. Vavilov Institute of Plant Industry, B. Morskaya Str., 42/44, 190000, St. Petersburg, Russia

    T. Gavrilenko

  2. Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190 Gross, Lüsewitz, Germany

    R. Thieme

  3. Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Protection in Field Crops and Grassland, Messeweg11/12, D-38104, Braunschweig, Germany

    U. Heimbach

  4. BTL Bio-Test Labor GmbH Sagerheide, Birkenallee 19, D-18184, Sagerheide, Germany

    Th. Thieme

Authors
  1. T. Gavrilenko
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  2. R. Thieme
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  3. U. Heimbach
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  4. Th. Thieme
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Gavrilenko, T., Thieme, R., Heimbach, U. et al. Fertile somatic hybrids of Solanum etuberosum (+) dihaploid Solanum tuberosum and their backcrossing progenies: relationships of genome dosage with tuber development and resistance to potato virus Y. Euphytica 131, 323–332 (2003). https://doi.org/10.1023/A:1024041104170

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