Abstract
Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in long-term callus tissue culture of Cereus peruvianus were studied in starch gel electrophoresis to investigate the control of differential Mdh gene expression under sugar and temperature stress. While two cytosol MDH isozymes showed an unchanged phenotype when the callus tissues were transferred to medium maintained at 22 or 37°C and containing different concentrations of sucrose, glucose, and fructose, the different combinations of five mitochondrial MDH (mtMDH) and two microbody MDH (mbMDH) showed different MDH isozyme patterns in the callus populations. Differential expression of mtMDH isozymes seems to be modulated at the posttranslational level in callus tissues exposed to different concentrations and types of sugar and to high-temperature and low-temperature stress. An inductor effect on the expression of mbMDH isozymes was observed under stress conditions and in long-term callus tissue, and they may also present different responses.
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REFERENCES
Bowler, C., Alliotte, T., De Loose, M., Van Montagu, M., and Inze, D. (1989). The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia. EMBO J. 8:31.
Chan, J. W. Y., and Burton, R. S. (1992). Variation in alcohol dehydrogenase activity and flood tolerance in white clover, Trifolium repens. Evolution 46:721.
Conklin, P. L., and Last, R. L. (1995). Differential accumulation of antioxidant mRNAs in Arabidopsis thaliana exposed to ozone. Plant Physiol. 109:203.
Cushman, J. C. (1992). Characterization and expression of a NADP-malic enzyme cDNA induced by salt stress from the facultative crassulacean acid metabolism plant, Mesembryanthemum crystallinum. Eur. J. Biochem. 208:259.
Daniel, P. P., Bryant, J. A., and Woodward, F. I. (1984). Phosphoenolpyruvate carboxylase from pennywort (Umbilicus rupestris). Changes in properties after exposure to water stress. Biochem J. 218:387.
del Rio, L. A., Sevilla, F., Sandalio, L. M., and Palma, J. M. (1991). Nutritional effect and expression of SODs: Induction and gene expression; Diagnostics; prospective protection against oxygen toxicity. Free Radic Res. Commun. 12–13(2):819.
Gamborg, O. L., Miller, K. A., and Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cell. Exp. Cell Res. 50:151.
Jacobs, M., Dolferus, R., and Van Den Bossche, D. (1988). Isolation and biochemical analysis of ethyl methanesulfonate-induced alcohol dehydrogenase null mutant of Arabidopsis thaliana (L.) Heynh. Biochem. Genet. 26:105.
Larkin, P. J., and Scowcroft, W. R. (1981). Somaclonal variation—A novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60:197.
Li, B., and Chollet, R. (1994). Salt induction and the partial purification/characterization of phosphoenolpyruvate carboxylase protein-serine kinase from an inducible crassulacean-acid-metabolism (CAM) plant, Mesembryanthemum crystallinum L. Arch. Biochem. Biophys. 314:247.
Machado, M. F. P. S., Prioli, A. J., and Mangolin, C. A. (1993). Malate dehydrogenase (MDH; EC 1.1.1.37) isozymes in tissue and callus cultures of Cereus peruvianus (Cactaceae). Biochem. Genet. 31:167.
Mangolin, C. A., Prioli, A. J., and Machado, M. F. P. S. (1997). Isozyme variability in plants regenerated from calli of Cereus peruvianus (Cactaceae). Biochem. Genet. 35:189–204.
McKersie, B. D., Chen, Y., de Beus, M., Bowley, S. R., Bowler, C., Inze, D., D'Halluin, K., and Botterman, J. (1993). Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L.). Plant Physiol. 103:1155.
Murashige, T., and Skoog, F. A. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:474.
Oliveira-Collet, S. A., Machado, M. F. P. S., and Prioli, A. J. (1996). Maintenance and development of Cereus peruvianus Mill. (Cactaceae) callus tissues in cultures. Arq. Biol. Tecnol. 39:525.
Perl-Treves, R., and Galun, E. (1991). The tomato Cu, Zn superoxide dismutase genes are developmentally regulated and respond to light and stress. Plant Mol. Biol. 17:745.
Sachs, M. M., Freeling, M., and Okimoto, R. (1980). The anaerobic proteins in maize. Cell 20:761.
Schaeffer, H. J., Forstheoefel, N. R., and Cushman, J. C. (1995). Identification of enhancer and silencer regions involved in salt-responsive expression of Crassulacean acid metabolism (CAM) genes in the facultative halophyte Mesembryanthemum crystallinum. Plant Mol. Biol. 28:205.
Tanksley, S. D., and Jones, R. A. (1981). Effects of O2 stress on tomato alcohol dehydrogenase activity: Description of a second ADH coding genes. Biochem. Genet. 19:397.
Torquato, E. F. B., Prioli, A. J., and Machado, M. F. P. S. (1995). Differential alcohol dehydrogenase and malate dehydrogenase isozyme expression in long-term callus tissue cultures of Cereus peruvianus (Cactaceae). Biochem. Genet. 33:389.
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Jorge, I.C., Mangolin, C.A. & Machado, M.F.P.S. Malate Dehydrogenase Isozymes (MDH; EC 1.1.1.37) in Long-Term Callus Culture of Cereus peruvianus (Cactaceae) Exposed to Sugar and Temperature Stress. Biochem Genet 35, 155–164 (1997). https://doi.org/10.1023/A:1021950008544
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DOI: https://doi.org/10.1023/A:1021950008544