Abstract
Adventitious stipular bud formation occurred in vitro in many strawberry cultivars during the proliferation phase onmedium containing Knop macronutrients, MS micronutrients, vitamins, aminoacids,2.22 μM BAP, 2.46 μM IBA and 0.29μM GA3. As described previously for cultivarGorella,cultivar Elsanta also showed adventitious stipular buds developing on theabaxial median zone between the stipule tips. To compare the shoots producedfrom both types of buds, clonal propagation was initiated from stipular budsandfrom axillary buds on the above mentioned medium. Stipular buds were separatedfrom the meristem-tip initiated plantlet and cultivated in the presence of alower BAP concentration (1.33 μM) to prevent further stipularbud formation.
During proliferation cycles, stipular originated propagules werevery easily distinguished by their specific leaf phenotype and light greencolour in comparison to plantlets cloned for an axillary bud. Theirmultiplication rate and cytokinin content were also higher than for axillarybuds. No significant difference was observed in auxin content.
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Jemmali, A., Elloumi, N., Kevers, C. et al. Morphological and hormonal characterisation of strawberry vitroplants raised through axillary or stipular adventitious shooting. Plant Growth Regulation 38, 273–278 (2002). https://doi.org/10.1023/A:1021526808514
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DOI: https://doi.org/10.1023/A:1021526808514