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Molecular characterization and evaluation of pectinase and cellulase production of Penicillium spp.

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Abstract

Penicillium species were analyzed with molecular markers and for pectinase and cellulase production. RAPD and PCR-RFLP analysis indicated high polymorphism among at least 5 of 10 Penicillium species. Five species were chosen for pectinase and cellulase production in liquid medium and four of which appeared similar based on molecular analyses. P. brevicompactum and P. griseoroseum gave the highest pectinase production and were highly divergent by molecular techniques.

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References

  • Alaña A, Gabilondo A, Hernando F, Moragues MD, Dominguez JB, Llama MJ, Serra JL (1989) Pectin lyase production by a Penicillium italicum strain. Appl. Environ. Microbiol. 55: 1612-1616.

    Google Scholar 

  • Albersheim P (1966) Pectin lyase from fungi. In: Neufeld EF, Ginsburg V, eds. Methods in Enzymology, Vol. 8. New York: Academic Press, pp. 628-631.

    Google Scholar 

  • Baracat MC, Valentin C, Muchovej JJ, Silva DO (1989) Selection of pectinolytic fungi for degumming of natural fibers. Biotechnol. Lett. 11: 899-902.

    Google Scholar 

  • Boysen M, Skouboe P, Frisvad J, Rossen L (1996) Reclassification of the Penicillium roqueforti group into three species on the basis of molecular genetic and biochemical profiles. Microbiology 142: 541-549.

    Google Scholar 

  • Brimer L, Cicalini AR, Federici F, Petruccioli M (1994) Production of β-glycosidases (linamarase and amygdalase) and pectolytic enzymes by Penicillium spp. World J. Microbiol. Biotechnol. 10: 203-206.

    Google Scholar 

  • Brumano MHN, Coelho JLC, Araújo EF, Silva DO (1993) Pectin lyase activity of Penicillium griseoroseum related to degumming of ramie. Rev. Microbiol. 24: 175-178.

    Google Scholar 

  • Cruz CD (2001) Programa GENES-versão Windows. Viçosa: Editora UFV.

    Google Scholar 

  • Cruz CD, Regazzi AJ (1994) Modelos biométricos aplicados ao melhoramento genético. Viçosa: Imprensa Universitária.

    Google Scholar 

  • Dupont J, Magnin S, Marti A, Brousse M (1999) Molecular tools for identification of Penicillium starter cultures used in the food industry. Int. J. Food Microbiol. 49: 109-118.

    Google Scholar 

  • Durand N, Reymond P, Fèvre M (1993) Randomly amplified polymorphic DNAs assess recombination following an induced parasexual cycle in Penicillium roqueforti. Curr. Genet. 24: 417-420.

    Google Scholar 

  • Kashyap DR, Vohra PK, Chopra S, Tewari R (2001) Applications of pectinases in the commercial sector: a review. Biores. Technol. 77: 215-227.

    Google Scholar 

  • Lobuglio KF, Taylor JW (1995) Phylogeny and PCR identification of the human pathogenic fungus Penicillium marneffei. J. Clin. Microbiol. 33: 85-89.

    Google Scholar 

  • Lobuglio KF, Pitt JI, Taylor JW(1993) Phylogenetic analysis of two ribosomal DNA regions indicates multiple independent losses of a sexual Talaromyces state among asexual Penicillium species in subgenus Biverticillium. Mycologia 85: 592-604.

    Google Scholar 

  • Nei M, Li W (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273.

    Google Scholar 

  • Paterson RRM, Bridge PD, Crosswaite MJ, Hawksworth DL (1989) A reappraisal of the Terverticillate Penicillia using biochemical, physiological and morphological features III. An evaluation of pectinase and amylase isoenzymes for species characterization. J. Gen. Microbiol. 135: 2979-2991.

    Google Scholar 

  • Pitt JI (1995) Phylogeny in the genus Penicillium: a morphologist's perspective. Can. J. Bot. 73: S768-S777.

    Google Scholar 

  • Pitt JI, Hocking AD (1999) Fungi and Food Spoilage, 2nd edn. Maryland: Aspen.

    Google Scholar 

  • Sequerra J, Marmeisse R, Valla G, Normand P, Capellano A, Moiroud A (1997) Taxonomic position and intraspecific variability of the nodule forming Penicillium nodositatum inferred from RFLP analysis of the ribossomal intergenic spacer and random amplified polymorphic DNA. Mycol. Res. 101: 465-472.

    Google Scholar 

  • Sneath PHA, Sokal RR (1973) Numerical Taxonomy. San Francisco: Freeman.

    Google Scholar 

  • Speacht CA, DiRusso CC, Novotny CP, Ullrich RC (1982) A method for extracting high-molecular-weight deoxyribonucleic acid from fungi. Anal. Biochem. 119: 158-163.

    Google Scholar 

  • Statsoft, Inc. (1995) STATISTICA for Windows (computer program manual). Tulso, Oklahoma.

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribossomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. San Diego: Academic Press, pp. 315-322.

    Google Scholar 

  • Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acid Res. 18: 6531-6535.

    Google Scholar 

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Pereira, J.F., Vieira de Queiroz, M., Aparecida Gomes, E. et al. Molecular characterization and evaluation of pectinase and cellulase production of Penicillium spp.. Biotechnology Letters 24, 831–838 (2002). https://doi.org/10.1023/A:1015502721909

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