Abstract
The influence of explant preparation on culture initiation and regeneration was investigated. Explant preparation was defined as the application of surface disinfection and homogenization to ginseng roots and embryos. Surface disinfection significantly affected culture initiation and subsequent embryogenesis. A high success rate (80–97%) in culture initiation was associated with explants from non-surface disinfected root tissue. The cumulative contamination rate after 8 months was below 4%; in contrast, a contamination rate of 85% was observed in disinfected root explants. For non-disinfected explants, visible callus was induced in 1 week, adventitious root and somatic embryos were formed in 2 and 6 months, respectively. Explants from disinfected roots required 1 month to induce visible callus, 5–7 months for adventitious roots and 10 months for somatic embryogenesis. For germinating embryos, disinfected embryos required double the time for embryogenesis than non-disinfected ones. It was considered that surface disinfection imposed a stress and subsequently a carry-over effect on explants.
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References
Barrett C & Cassells AC (1994) An evaluation of antibiotics for the elimination of Xanthomonas campestris pv. Pelargonii (Brown) from Pelargonium x domesticum cv. ?rand Slam’ explants in vitro. Plant Cell Tiss. Org. Cult. 36: 169–175
Cassells AC (1991) Problem in tissue culture: culture contamination. In: Debergh PC & Zimmerman RH (eds) Micropropagation: Technology and Application (pp 31–44). Kluwer Academic Publishers, Dordrecht, London
Chang WC & Hsing YI (1980) Plant regeneration through somatic embryogenesis in root-derived callus of ginseng (Panax ginseng C.A. Meyer). Theor. Appl. Genet. 57: 133–135
Gilbert JE, Shohet S & Caligari PDS (1991) The use of antibiotics to eliminate latent bacterial contamination in potato cultures. Ann Appl. Biol. 119: 113–120
Gunson HE & Spencer-Philips PTN (1994) Latent bacterial infections: Epiphytes and endophytes as contaminants of micropropagated plants. In: Nicholas JR (ed) Physiology, Growth and Development of Plants in Culture (pp 379–396). Kluwer Academic Publishers, Dordrecht, Netherlands
Leifert C, Camotta H & Waites WM (1994) Effect of combinations of antibiotics on micropropagated Clematis, Delphinium, Hosta, Iris and Photinia. Plant Cell Tiss. Org. Cult. 29: 153–160
Minocha SC (1985) In vitro propagation of Dionaea muscipula. HortScience 20: 216–217
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497
Pierik RLM (1989) Sterilization of plant material. In: Pierik RLM (ed) In Vitro Culture of Higher Plants (pp 87–94). Martinus Nijhoff Publ. Dordrecht
Reed BM & Tanprasert P (1995) Detection and control of bacterial contaminants of plant tissue cultures. A review of recent literature. Plant Tiss Cult. Biot. 1: 137–142
Reed BM, Buckley PM & DeWilde TN (1995) Detection and eradication of endophytic bacteria from micropropagated mint plants. In Vitro Cell. Dev. Biol. 31: 53–57
Teng WL, Liu YJ, Tsai YC & Soong TS (1994) Somatic embryo-genesis of carrot in bioreactor culture system. HortScience 29: 1349–1352
Teng WL, Nicholson L (1997) Pulse treatments of penicillin-G and streptomycin minimise internal infections and have post-treatment effects on the morphogenesis of ginseng root culture. Plant Cell Rep. 16: 531–535
Teng WL & Teng MC (2000) The impact of pulse treatment of penicillin-G and streptomycin sulfate on sporophyte regeneration of Platycerium bifurcatum. Plant Cell Rep. 19: 971–977
Torres KC (1989) Basic techniques and principles. In: Tissue Culture Techniques for Horticultural Crops (pp 1–70). Published by Van Nostrand Reinhold, New York
Wilson ZA & Power JB (1989) Elimination of systemic contamination in explant and protoplast cultures of rubber (Hevea brasiliensis Muell. Arg.). Plant Cell Rep. 7: 622–625
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Teng, WL., Sin, T. & Teng, MC. Explant preparation affects culture initiation and regeneration of Panax ginseng and Panax quinquefolius . Plant Cell, Tissue and Organ Culture 68, 233–239 (2002). https://doi.org/10.1023/A:1013957423655
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DOI: https://doi.org/10.1023/A:1013957423655