Abstract
Calli cultures derived from the leaves of Saussurea medusa were selected on the basis of colour into three callus, A, B and C, which suggested different levels of metabolite accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the jaceosidin content of these samples. The jaceosidin concentration in callus B was higher than that of the callus A and C. By using 12-day old culture and 9-day old inoculum, jaceosidin yield of 72.91 mg l−1was obtained from cell line B in cell suspension cultures. The influence of some factors affecting jaceosidin formation, i.e. temperature, light, inoculum size, type of media, phytohormones, nitrogen and carbon source etc. were also examined. Light irradiation and combination of 3% (w/v) sucrose with 1% glucose brought about a marked increase of jaceosidin production. The effect of blue light on jaceosidin was markedly superior to other kinds of monochromatic light (red and far-red) or white light. Analysis of growth and jaceosidin content of callus cultures and cell suspension cultures demonstrated that the production of jaceosidin was growth-dependent in both cell solid culture and cell suspension culture.
Similar content being viewed by others
References
Abdullah MA, Ali AM, Marziah M, Lajis NH & Ariff AB (1998) Establishment of cell suspension cultures of Morinda elliptica for the production of anthraquinones. Plant Cell Tissue and Organ Culture 54: 173–182
Bourgin JP, Nitsch JP (1967) Obtention de nicotiana haploides a partir detamines cultivees in vitro. Ann. Physiol. Veg. 9: 377–382
Delectis Florae Popularis Sinicae Agendae Academiae Sinicae Edita (1999) Flora Reipublicae Popularis Sinicae, Tomus 78 (2) (pp 1–2). Sciences Press, Beijing
Ensminger PA & Schaefer E (1992) Blue and Ultraviolet-B light photoreceptors in parsley cells. Photochem. Photobiol. 55: 437–447
Fu LK & Jin JM (1992) China Plant Red Data Book: Rare and Endangered Plants Vol. 1 (pp 234–235). Science Press, Beijing-New York
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Gil B, Sanz MJ, Terencio MC, Feerandiz MJL, Bustos G, Paya M, Gunasegaran R & Alcaraz MJ (1994) Effect of flavonoids on naja naja and human recombinant synovial phospholipases A2 and inflammatory responses in mice. Life Sci. 54: 333–338
Li GH, Liu F & Xin Z (1979) Studies on pharmacodynamic actions in Saussurea medusa. Chin. Bull. Pharma. 14: 86–87
Lian WY & Xiao PG (1985) The investigation of Subgen. Eriocoryne (DC.) Hook Trad. Chin. Med. 6: 19
Liu LS, Xiao XH & Zhang LD (1985) Effect of the flavonoids from Saussurea involucrata on DNA synthesis of cancer cells. J. Lanzhou Unive Natu Scie 21: 80–83
Matsumoto T, Nishida K, Noguchi M & Tamaki E (1973) Some factors affecting the anthocyanin formation by Populus cells in suspension culture. Agr. Biol. Chem. 37: 561–567
Miller CO (1963) Kinetin and kinetin-like compounds in modern methods of plant analysis. Springer, Berlin-Heidelberg-New York 6: 194–202
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissure cultures. Physiol. Planta 15: 473–497
Nitsch JP (1951) Growth and development in vitro of excised ovaries. Am. J. Bot. 38: 566–577
Schinella GR, Giner RM, Recio MC, Mordujovich De Buschiazzo P, Rios JL & Manez S Catedra De Farmacologia (1998) Antiinflammatory effects of south American Tanacetum vulgare. J. Pharm. Pharmacol. 50: 1069–1074
Seo WT, Park YH & Choe TB (1993) Identification and production of flavonoids in cell suspension culture of Scutellaria baicalensis G. Plant Cell Rep. 12: 414–417
White PR (1963) The Cultivation of Plant and Animal Cells. 2nd edn (p. 57). Onald Press, New York
Woerdenbag HJ, Merfort I, Parßeiter CM, Schmidt TJ, Willuhn G, Van Uden W, Pras N, Kampinga HH & Konings AWT (1994) Cytotoxity of flavonoids and sesquiterpene lactones from Arnica species against the GLC4 and the COLO 320 cell lines. Planta Med 60: 434–437
Yamakawa T, Kato S, Ishide K, Kodama T & Minoda Y (1983) Production of anthocyanins by vitis cells in suspension culture. Agr. Biol. Chem. 47: 2185–2191
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhao, D., Xing, J., Li, M. et al. Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa . Plant Cell, Tissue and Organ Culture 67, 227–234 (2001). https://doi.org/10.1023/A:1012770623153
Issue Date:
DOI: https://doi.org/10.1023/A:1012770623153