Abstract
To ensure that narrow-leafed lupin (Lupinus angustifolius L.) meets feed quality standards, the concentration of alkaloids must be kept under the maximum acceptable limit of 200 mg kg−1 DM. One of the factors that may affect seed alkaloid concentration is soil nutrient deficiency. In this paper, we report the results of glasshouse and field experiments that tested the effect of potassium (K) deficiency on seed alkaloid concentrations. In the glasshouse, seed alkaloid concentrations increased by 385, 400 and 205% under severe K deficiency in sweet varieties (Danja, Gungurru and Yorrel, respectively) of L. angustifolius. The concentration of alkaloids in Fest, the bitter variety, was always high regardless of soil K status. At all levels of applied K (0–240 mg kg−1 soil), lupanine was the predominant alkaloid in sweet varieties, whereas 13-hydroxylupanine prevailed in the bitter variety. Seed yield of all varieties increased exponentially with increasing amounts of applied K, reaching a maximum at 60 mg K kg−1 soil. In the field, application of K to deficient soils decreased seed alkaloid concentration at Badgingarra, Western Australia (WA) but not at Nyabing, WA, in 1996. In both field trials, seed yield and mineral content were not affected by the amounts of K fertiliser applied. These findings highlighted the need for adequate K fertilisation of deficient soils in WA to avoid the risk of producing low quality lupin seed with high alkaloid concentrations. K deficiency is involved in stimulating alkaloid production in sweet varieties of L. angustifolius.
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References
Allen D G, Greirson B N, Harris D J and Spadek Z E 1990 Alkaloid profiles of various lupin species. Chemistry Centre Western Australia, Occasional Report No. 89B28804-55, Perth, WA. 13 p.
AOAC 1999 Official Methods of Analysis of AOAC International. 16th Edition, 5th revision, Method 4.2.04 (968.06). Ed. P Cunniff. p 13. AOAC International, Gaithersburg, MA, USA.
Bar-Tal A 1999 The significance of root size for plant nutrition in intensive horticulture. In Mineral Nutrition of Crops – Fundamental Mechanisms and Implications. Ed. Z Rengel. pp 115–139. Food Product Press® The Haworth Press Inc, Birghmanton, N Y.
Connellan G 1996 The pursuit of optimum environments in Australian greenhouses. Eds. C Kubota, K Fujiwara, Y Ibaraki and S Sase. pp 159–164. Proceedings of the International Symposium on Plant production in closed ecosystems. Acta Hort. 440, August 1996. ISHS, Tokyo, Japan.
Cox W J 1978 Potassium deficiency in lupins – identification, rates, times and method of application. J. Agric. West. Aust.19, 27–31.
Croteau R, Kutchan T M and Lewis N G 2000 Natural products (secondary metabolites). In Biochemistry andMolecular Biology of Plants. Eds. B Buchanan, W Gruissem and R Jones. pp 1250–1318. American Society of Plant Physiologists, Rockville, MD USA
Culvenor C C J and Petterson D S 1986 Lupin toxins – alkaloids and phomopsins. pp. 188–198. Proceedings of the 4th International Lupin Conference, August 1986. Dept Agric.WA, Perth, WA.
Dracup M and Kirby E J M 1996 Lupin development guide. U WA Press, Perth, WA, Australia. 97 p.
Edwards N K 1993 Distribution of potassium in the soil profile of a sandplain soil under pasture species. Plant Soil 155, 407–410.
Edwards N K 1995 Potassium nutrition of lupins in the sandplain soils of Western Australia. Final Rep. Plant Ind. Div., Dept Agric.WA, Perth, WA. 13 p.
Genstat 5 Committee 1993 Genstat 3 Reference Manual, Clarendon Press, Oxford, UK. 796 p.
Gladstones J S 1970 Lupins as crop plants. Field Crop Abs. 23, 2, 123–148.
Gremigni P, Hamblin J and Harris D 1997 Alkaloid level in lupin seed is affected by nutritional stresses. Proceedings of the International Food Legume Research Conference III, September1997. Adelaide, SA. 143 p.
Gremigni P, Gazey C, Hamblin J and Harris D 1998 Soil nutritional status affects alkaloid levels in Australian sweet lupin. Proceedings of the 3rd European Conference on Grain Legumes, November 1998. AEP, Paris, France. 179 p.
Gremigni P, Hamblin J and Harris D 1999 Genotype ?environment interactions and lupin alkaloids. Eds. E Van Santen, M Wink, S Weissman and P Romer. pp 362–365. Lupin – An ancient crop for the new millennium. Proceedings of the 9th International Lupin Conference, June 1999. Klink-Müritz, Germany.
Hamblin A P and Hamblin J 1985 Root characteristics of some temperate legume species and varieties on deep, free-draining entisols. Aust. J. Agric. Res. 36, 63–72.
Harborne J B and Baxter H 1993 Phytochemical Dictionary. Eds. Taylor & Francis. pp 284–289. London, UK.
Harris D J 1994 Quality production and breeding. Eds. M Dracup and J Palta. pp 98–105. Proceedings of the 1st Australian Lupin Technical Symposium, October 1994. Dept Agric.WA, Perth, WA.
Harris D and Wilson P E 1988 A rapid manual method of lupin alkaloid analysis. Ed. T Twardowski. pp 598–601. Proceedings of the 5th International Lupin Conference, July 1988. Poznan, Poland.
Hartmann T 1988 Secondary metabolism of lupins: biosynthesis, translocation and accumulation of the quinolizidine alkaloids. Ed. T Twardowski. pp 64–78. Proceedings of the 5th International Lupin Conference. Poznan, Poland.
Lowen R, Alam F K A and Edgar J A 1995 Lupin bean toxicity. Med. J. Aust. 162, 256–257.
McQuaker N R, Brown D F and Fluckner P D 1979 Digestion of environmental materials for analysis by inductively coupled plasma-atomic emission spectrometry. Anal. Chem. 51, 1082–1084.
Marschner H 1995 Functions of mineral nutrients: macronutrients. In Mineral Nutrition of Higher Plants. Ed. H Marschner. pp 299–312. Academic Press, London, UK.
Northcote K H 1971 A factual key for the recognition of the Australian soils. Rellim Glenside, S A. 123 p.
Nowacki E K and Waller G R 1970 Quinolizidine alkaloids from Leguminosae. Rev. Latinoamer. Quím. 8, 49–56.
Perry M W, Dracup M, Nelson P, Jarvis R, Rowland I and French R J 1998 Agronomy and farming systems. In Lupins as Crop Plants: Biology, Production and Utilisation. Eds. JS Gladstones, CA Atkins and J Hamblin. pp 291–338. CAB International, Wallingford, UK.
Petterson D S, Sipsas S and Mackintosh J B 1997 The chemical composition and nutritional value of Australian pulses. pp 7–9. Grains Research & Development Corporation, Canberra, ACT.
Priddis C R 1983 Capillary gas chromatography of lupin alkaloids. J. Chrom. 261, 95–101.
Rana J and Robins D J 1985 Biosynthesis of quinolizidine alkaloids, incorporation of [1-amino-15N,1-13C] cadaverine into lupanine, 13-hydroxylupanine, and angustifoline.J. Chem. Res. (S), 196–197.
Rayment G E and Higginson F R 1992 Australian laboratory handbook for soil and water chemical methods. Australian soil and land survey handbook. Inkata Press, Melbourne, VIC, Australia. 330 p.
Robins D J 1987 Synthesis of [1,2-13C2] cadaverine and its incorporation into five quinolizidine alkaloids. J. Chem. Res. (S), 256–257.
Rowland I C, Mason M G and Hamblin J 1986 Effects of lupins on soil fertility. pp 96–111. Proceedings of the 4th International Lupin Conference, August 1986. Dept Agric.WA, Perth, WA.
Scibor-Marchocka A 1970 Comparative studies on the homologous types of bitter and fodder white lupine. Acta Agrobot. 23(1), 23–38.
Tang C 1998 Factors affecting soil acidification under legumes I. Effect of potassium supply. Plant Soil 199, 275–282.
Thomson B D and Perry M W 1995 A guide to selecting grain legumes for your soil. pp 1–8. CLIMA, Perth, WA.
Walkley A and Black I A 1934 an examination of the Degtjareffe method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci. 37, 29–38.
Waller G R and Nowacki E K 1978 Alkaloid biology and metabolism in plants. Plenum Press, New York. 294 p.
Walton G H and Francis C M 1975 Genetic influences on the split seed disorder in Lupinus angustifolius L. Aust. J. Agric. Res. 26, 641–646.
Wink M 1987 Quinolizidine alkaloids: Biochemistry, metabolism and function in plants and cell suspension cultures. Planta Med. 53, 509–514.
Wink M 2000a Functions of plant secondary metabolites and their exploitation in biotechnology. Vol. 3 of Annual Plant Reviews, Sheffield Academic Press. 362 p.
Wink M and Hartmann T 1982 Localisation of the enzymes of quinolizidine alkaloid biosynthesis in leaf chloroplasts of Lupinus polyphyllus. Plant Physiol. 70, 74–77.
Wong M T F, Edwards N K and Barrow N J 2000a Accessibility of subsoil potassium to wheat grown on duplex soils in the southwest of Western Australia. Aust. J. Agric. Res. 38, 745–751.
Wong M T F, Gilkes R J, Edwards N K and Pal Y 2000b Using recently acquired knowledge to manage potassium sustainability in the wheatbelt. pp 86–93. Proceedings of Soils 2000, Muresk Institute of Agriculture, July 2000. Northam, WA, Australian Society of Soil Science Inc. (WA Branch).
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Gremigni, P., Wong, M.T.F., Edwards, N.K. et al. Potassium nutrition effects on seed alkaloid concentrations, yield and mineral content of lupins (Lupinus angustifolius). Plant and Soil 234, 131–142 (2001). https://doi.org/10.1023/A:1010576702139
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DOI: https://doi.org/10.1023/A:1010576702139