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Evidence for polynuclear iron(III) clusters in the root nodule bacterium, Rhizobium leguminosarum bv. viciae WSM710

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Abstract

Cells of the root nodule bacterium Rhizobium leguminosarum bv. viciae WSM710 were cultured in a medium containing 20 μM 57Fe. Mössbauer spectra of the cells at 5.5 and 3.7 K indicated that the major form of iron present in the cells was in the form of polynuclear iron(III) clusters. At 5.5 K the spectral component associated with these clusters was in the form of a superposition of a broad feature (large magnetic hyperfine field distribution) and a doublet. On lowering the temperature of the cells to 3.7 K, the spectral component was transformed into resolved magnetic hyperfine field splitting which yielded a magnetic hyperfine field of 42.4 T when fitted with broad Lorentzian peaks. These spectral characteristics are typical of the hydrated iron(III) phosphate cores of several bacterioferritins. A small fraction (11%) of the Mössbauer spectral area of the cells was in the form of a doublet which yielded parameters (δ = 1.35 mm/s; ΔEQ = 3.15 mm/s) indicative of iron(II). The parameters are very similar to those of a spectral component previously observed in several other microbes (R. Böhnke and B.F. Matzanke (1995) BioMetals 8, 223-230) and which has been associated with a 2.2 kDa oligomeric iron(II) carbohydrate phosphate

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References

  • Andrews SC. 1998 Iron storage in bacteria. In: Poole, R, eds. Advances in Microbial Physiology, vol 40. London: Academic Press

    Google Scholar 

  • Bauminger ER, Cohen SG, Dickson DPE, Levy A, Ofer S, Yariv J. 1980 Mössbauer spectroscopy of Escherichia coli and its iron-storage protein. Biochim Biophys Acta 623, 237–242

    Google Scholar 

  • Böhnke R, Matzanke BF. 1995 The mobile ferrous pool in Escherichia coli is bound to a phosphorylated sugar derivative. Biometals 8, 223–230

    Google Scholar 

  • Brown CM, Dilworth MJ. 1975 Ammonia assimilation by Rhizobium cultures and bacteroids. J Gen Microbiol 86, 39–48

    Google Scholar 

  • Carson KC, Dilworth MJ, Glenn AR. 1992a Siderophore production and iron transport in Rhizobium leguminosarum bv. viciae MNF710. J Plant Nutr 15, 2203–2220

    Google Scholar 

  • Carson KC, Holliday S, Glenn AR, Dilworth MJ. 1992b Siderophore and organic acid production in root nodule bacteria. Arch Microbiol 157, 264–271

    Google Scholar 

  • Carson KC, Glenn AR, Dilworth MJ. 1994 Specificity of siderophore mediated transport of iron in rhizobia. Arch Microbiol 161, 333–339

    Google Scholar 

  • Dilworth, MJ and Glenn, AR 1991 The biology and biochemistry of nitrogen fixation: a look forward. In The biology and biochemistry of nitrogen fixation (Eds. M.J. Dilworth and A.R. Glenn) Elsevier, Amsterdam pp 1–18.

    Google Scholar 

  • Dilworth MJ, Carson KC, Giles RGF, Byrne LT, Glenn AR. 1998 Rhizobium leguminosarum bv. viciae produces a novel cyclic trihydroxamate siderophore, vicibactin. Microbiology 144, 781–791

    Google Scholar 

  • Gill PR, Neilands JB. 1989 Cloning a genomic region required for a high-affinity iron-uptake system in Rhizobium meliloti 1021. Mol Microbiol 3, 1183–1189

    Google Scholar 

  • Guerinot ML, Meidl EJ, Plessner O. 1990 Citrate as a siderophore in Bradyrhizobium japonicum. J Bacteriol 172, 3298–3303

    Google Scholar 

  • Hemantaranjan A, Garg OK. 1986 Introduction of nitrogen fixing nodules through iron and zinc fertilization in the non nodule-forming French bean (Phaseolus vulgaris L). J Plant Nutr 9, 281–288

    Google Scholar 

  • Hudson AJ, Andrews SC, Hawkins C, Williams JM, Izuhara M, Meldrum FC, Mann S, Harrison PM, Guest JR. 1993 Overproduction, purification and characterization of the Escherichia coli ferritin. Eur J Biochem 218, 985–995

    Google Scholar 

  • Jadhav RS, Desai A. 1994 Role of siderophore in iron uptake in cowpea Rhizobium GN1 (peanut isolate) — possible involvement of iron repressible outer membrane proteins. FEMS Microbiol Lett 115, 185–189

    Google Scholar 

  • Matzanke BF, Bill E, Trautwein AX. 1992 Main components of iron metabolism in microbial systems — analysed by in vivo Mössbauer spectroscopy. Hyp Int 71, 1259–1262

    Google Scholar 

  • Modi M, Shah KS, Modi VV. 1985 Isolation and characterisation of catechol-type siderophore from cowpea Rhizobium RA-1. Arch Microbiol 141, 156–158

    Google Scholar 

  • Nambiar PTC, Sivaramakrishnan S. 1987 Detection and assay of siderophores in cowpea rhizobia (Bradyrhizobium) using radioactive Fe (59Fe). Appl Micro Lett 4, 37–40

    Google Scholar 

  • O'Hara GW, Dilworth MJ, Bookerd N, Parkpian P. 1988 Iron deficiency specifically limits nodule development in peanut inoculated with Bradyrhizobium sp. New Phytol 108, 51–57

    Google Scholar 

  • Patel HN, Chakraborty RN, Desai SB. 1988 Isolation and partial characterization of phenolate siderophore from Rhizobium leguminosarum IARI102. FEMS Microbiol Lett 56, 131–134

    Google Scholar 

  • Persmark M, Pittman P, Buyer JS, Schwyn B, Gill PR, Neilands JB. 1993 Isolation and structure of rhizobactin 1021, a siderophore from alfalfa symbiont Rhizobium meliloti 1021. J Am Chem Soc 115, 3950–3956

    Google Scholar 

  • Rai R, Singh SN, Prasad V. 1982 Effect of pressmud amended pyrite on symbiotic N2-fixation, active iron content of nodules, grain yield and quality of chickpea (Cicer arietinum Linn.) genotypes in calcareous soil. J Plant Nutr 5, 905–913

    Google Scholar 

  • Rioux CR, Jordan DC, Rattray JBM. 1986a Iron requirement of Rhizobium leguminosarum and secretion of anthranilic acid during growth on an iron-deficient medium. Arch Biochem 248, 175–182

    Google Scholar 

  • Roy N, Bhattacharyya P, Chakrabartty PK. 1994 Iron acquisition during growth in an iron deficient medium by Rhizobium sp. isolated from Cicer arietinum. Microbiology 140, 2811–2820

    Google Scholar 

  • St. Pierre TG, Bell SH, Dickson DPE, Mann S, Webb J, Moore GR, Wiliams RJP. 1986 Mössbauer spectropic studies of the cores of human, limpet and bacterial ferritins. Biochim Biophys Acta 870, 127–134

    Google Scholar 

  • St. Pierre TG, Webb J, Mann S. 1989 Ferritin and Hemosiderin: structural and magnetic studies of the iron core. In: Mann, S, Webb, J, Williams, RJP, eds Biomineralization, chemical and biochemical perspectives. Weinheim: VCH Publishers

    Google Scholar 

  • Tang C, Robson AD, Dilworth MJ. 1991 Which stage of nodule initiation in Lupinus angustifolius L. is sensitive to iron deficiency? New Phytol 117, 243–250

    Google Scholar 

  • Yariv J, Kalb AJ, Sperling R, Bauminger ER, Cohen SG, Ofer S. 1981 The composition and the structure of bacterioferritin of Escherichia coli. Biochem J 197, 171–175

    Google Scholar 

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St. Pierre, T.G., Carson, K.C., Webb, J. et al. Evidence for polynuclear iron(III) clusters in the root nodule bacterium, Rhizobium leguminosarum bv. viciae WSM710. Biometals 12, 73–76 (1999). https://doi.org/10.1023/A:1009256525195

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