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Copper sensitizes the mitochondrial permeability transition to carboxyatractyloside and oleate

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Abstract

Addition of 5 μM copper to rat kidney mitochondria enhances the effect of carboxyatractyloside and oleate on pore opening, in a cyclosporin A-sensitive fashion. The effects of the pair copper-carboxyatractyloside were observed on matrix Ca2+ efflux, mitochondrial swelling and on the transmembrane electric gradient. The effect of Cu2+ emphasizes the importance of membrane thiol groups located, probably, in the ADP/ATP translocase (ANT), on permeability transition. It was also found that Cu2+ does not block the fluorescent label of ANT by eosin 5-maleimide, but abolishes the inhibition by CAT on the labeling. This suggests that the binding of Cu2+ to cysteine residues of ANT promotes a conformational change in the carrier, strengthening the effect of CAT and oleate on membrane leakage.

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Authors and Affiliations

  1. Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, México, D.F., 014080, México

    Noemí García, Cecilia Zazueta, Raymundo Carrillo, Francisco Correa & Edmundo Chávez

Authors
  1. Noemí García
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  2. Cecilia Zazueta
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  3. Raymundo Carrillo
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  4. Francisco Correa
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  5. Edmundo Chávez
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García, N., Zazueta, C., Carrillo, R. et al. Copper sensitizes the mitochondrial permeability transition to carboxyatractyloside and oleate. Mol Cell Biochem 209, 119–123 (2000). https://doi.org/10.1023/A:1007151511817

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