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Simultaneous up-regulation of urokinase-type plasminogen activator (uPA) and uPA receptor by hepatocyte growth factor/scatter factor in human glioma cells

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Abstract

Several lines of evidence indicate that hepatocyte growth factor/scatter factor (HGF/SF) and its receptor, c-Met, may play an important role in progression of human glioma. In this study, effects of HGF/SF on urokinase- type plasminogen activator (uPA)-mediated proteolysis network were examined in c-Met-positive human glioma cell lines. Treatment of the glioma cells with various concentrations of HGF/SF resulted in an enhanced secretion of uPA proteins accompanying increased transcription of uPA mRNA in a dose dependent fashion. The levels of uPA receptor (uPAR) mRNAs were also elevated simultaneously upon HGF/SF stimulation, and the cell-surface associated uPA activity was also elevated by the treatment. Since concomitant expression of HGF and its receptor c-Met are frequently observed in malignant gliomas, these results suggest that HGF/SF participates in invasive process of malignant glioma cells not only by its motility-stimulating activity but also through enhanced degradation of the extracellular matrix induced by autocrine activation of uPA proteolysis network.

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Authors and Affiliations

  1. Department of Neurosurgery, Japan

    Takuzou Moriyama, Ryouichi Hamasuna, Tetsuro Sameshima, Tsutomu Iseda, Kiyotaka Yokogami, Shinichi Nakano & Shinichiro Wakisaka

  2. Second Department of Pathology, Japan

    Hiroaki Kataoka & Masashi Koono

  3. Second Department of Physiology, Miyazaki Medical College, Miyazaki, Japan

    Etsuo Yoshida

Authors
  1. Takuzou Moriyama
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  2. Hiroaki Kataoka
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  3. Ryouichi Hamasuna
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  4. Etsuo Yoshida
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  5. Tetsuro Sameshima
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  6. Tsutomu Iseda
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  7. Kiyotaka Yokogami
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  8. Shinichi Nakano
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  9. Masashi Koono
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  10. Shinichiro Wakisaka
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Moriyama, T., Kataoka, H., Hamasuna, R. et al. Simultaneous up-regulation of urokinase-type plasminogen activator (uPA) and uPA receptor by hepatocyte growth factor/scatter factor in human glioma cells. Clin Exp Metastasis 17, 873–879 (1999). https://doi.org/10.1023/A:1006729611241

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  • DOI: https://doi.org/10.1023/A:1006729611241

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