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Anatomical and biochemical characterization of the calcium effect on Eucalyptus urophylla callus morphogenesis in vitro

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Abstract

Calcium chloride concentrations from 0.0 to 12.12 mM were added to the culture medium and calcium content in calluses were determined directly by X-ray fluorescence spectrometry, a non-destructive method, allowing the processing of the same tissue for histological analysis. A multivariate statistical analysis (PCA – Principal Components Analysis) grouped the treatments into 5 blocks and indicated the most responsive group. Lack of calcium supply caused a complete absence of a morphogenic process and tissue collapse. An increase in calcium concentration gave higher total protein and sugar contents, an increase in peroxidase specific activity and changes in the histological characteristics. It was possible to verify that calcium stimulated globular somatic embryo formation at concentration of 6.62 mM.

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Authors and Affiliations

  1. Departamento de Ciências Florestais, Escola Superior de Agricultura `Luíz de Queiroz', Universidade de São Paulo, PO Box 09, 13.418-900, Piracicaba, São Paulo, Brazil

    S.C.C. Arruda

  2. Departamento de Botânica, UNESP, Rio Claro, São Paulo, Brazil

    G.M. Souza

  3. Departamento de Ciências Biológicas, ESALQ/USP, Piracicaba, São Paulo, Brazil

    M. Almeida

  4. Departamento de Ciências Florestais, Escola Superior de Agricultura `Luíz de Queiroz', Universidade de São Paulo, PO Box 09, 13.418-900, Piracicaba, São Paulo, Brazil

    A.N. Gonçalves & A.N. Gonçalves

Authors
  1. S.C.C. Arruda
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  2. G.M. Souza
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  3. M. Almeida
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  4. A.N. Gonçalves
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Correspondence to A.N. Gonçalves.

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Arruda, S., Souza, G., Almeida, M. et al. Anatomical and biochemical characterization of the calcium effect on Eucalyptus urophylla callus morphogenesis in vitro . Plant Cell, Tissue and Organ Culture 63, 142–154 (2000). https://doi.org/10.1023/A:1006482702094

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