Abstract
A system for rapid plant regeneration through somatic embryogenesis from shoot tip explants of sorghum [Sorghum bicolor (L.) Moench] is described. Somatic embryogenesis was observed after incubation of explants in dark for 6–7 weeks through a friable embryogenic callus phase. Linsmaier and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (2 mg l−1) and kinetin (0.1 mg l −1) was used for induction of friable embryogenic calli and somatic embryos. Germination of somatic embryos was achieved about 5 weeks after transfer onto Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (2 mg l−1) and indole-3-acetic acid (0.5 mg l −1) under light. Seeds from in vitro-regenerated plants produced a normal crop in a field trial, and were comparable to the crop grown with the seeds of the mother plant used to initiate tissue culture. The simplicity of the protocol and possible advantages of the system for transformation over other protocols using different explants are discussed.
Similar content being viewed by others
References
Bhaskaran S& Smith RH (1988) Enhanced somatic embryogenesis in Sorghum bicolor from shoot-tip culture. In Vitro Cell Dev. Bio. 24: 65–70
Casas A, Kononowicz AM, Zehr BU, Tomes DT, Axtell JD, Butler LG& Bressan RA (1993) Transgenic sorghum plants via microprojectile bombardment. Proc. Nat. Acad. Sci. USA 90: 11212–11216
Elhag H& Butler LG (1992) Effect of genotype, explant age and medium composition on callus production and plant regeneration from immature embryos of sorghum. Arab Gulf. J. Sci. Res. 10: 109–119
Elkonin LA, Lopushanskaya RF& Pakhomova NV (1995) Initiation and maintenance of friable embryogenic callus of Sorghum Sorghum bicolor (L.) Moench by amino acids. Maydica 40: 153–157
Kaeppler HF& Pedersen JF (1997) Evaluation of 41 elite and exotic inbred sorghum genotypes for high quality callus production. Plant Cell Tiss. Org. Cult. 48: 71–75
Linsmaier EM& Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100–127
Murashige T& Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nahdi S& de Wet JMJ (1995) In vitro regeneration of Sorghum bicolor lines from shoot apices. Int. Sorghum&Millets Newsletter 36: 88–90
Rao AM, Padmasree K& Kavikishore P B (1995) Enhanced plant regeneration in grain and sweet sorghum by aspargine, proline and cefotaxime. Plant Cell Rep. 15: 72–75
Sairam RV, Seetharama N, Murthy AK& Shyamala T (1996) Comparison of tissue culture systems for genetic transformation in sorghum. Page 150 in Abstracts of poster sessions. 2nd International Crop Science Congress: Crop Productivity and Sustainability – Shaping the Future, 17–24 Nov 1996, New Delhi, India. New Delhi: National Academy of Agricultural Sciences; Indian Council of Agricultural Research
Sairam RV, Seetharama N, Devi PS, Verma A, Murthy UR& Potrykus I (1999) Plant regeneration from mesophyll protoplasts in sorghum [Sorghum bicolor (L.) Moench]. Plant Cell Rep. 18: 972–977.
Zhong Z, Srinivasan C& Sticklen M (1992) In vitro morphogenesis of corn: Differentiation of multiple shoot clumps and somatic embryos from shoot tips. Planta 187: 483–489
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Seetharama, N., Sairam, R. & Rani, T. Regeneration of sorghum from shoot tip cultures and field performance of the progeny1. Plant Cell, Tissue and Organ Culture 61, 169–173 (2000). https://doi.org/10.1023/A:1006403601498
Issue Date:
DOI: https://doi.org/10.1023/A:1006403601498