Abstract
Carbon 14-labelled indole-3-acetic acid (IAA) was fed to segments of shoots of Zea mays seedlings grown in light or dark to find the effect of light on IAA metabolism. The seedling parts coleoptile, with enclosed leaf, and mesocotyl were also used to examine differences in IAA metabolism between tissue types. The rate of metabolite formation as a function of time ranging from 1 to 12 hours was determined. Light did not significantly influence the amount of IAA taken up, but significantly increased its rate of metabolism and greatly increased the content of amide conjugates formed. There were also differences in metabolism depending on tissue type. In all tissues, IAA was metabolized mainly into six compounds. Four were tentatively identified as IAA-glucose (IAGlc), IAA-myo-inositol} (IAInos), indole acetamide (IAAm) and IAA-aspartic acid (IAAsp). 1-O-IAA-D-glucose (1-O-IAGlc) was the first conjugate formed and, except for mesocotyls in the light, it was the most abundant conjugate in maize tissue. In mesocotyl tissue the conversion of IAA into IAAsp was greatly stimulated by light, and the biosynthesis of IAAsp exceeded that of IAGlc. Since light strongly inhibited the growth of the mesocotyl, it is possible that the stimulation of IAAsp synthesis by light causes depletion of free IAA with resultant inhibition of mesocotyl growth.
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References
Bandurski RS and Schulze A (1977) Concentration of Indole-3–acetic acid and its derivatives in plants. Plant Physiol 60: 211–213
Bandurski RS, Schulze A, Desrosiers M, Jensen P, Epel B and Reinecke D (1990) Relationship between stimuli, IAA and growth. In: Pharis RP and Rood SB (eds) Plant Growth Substances 1988. Springer-Verlag, pp 341–352
Behringer FJ, Davies PJ, Yang T and Law DM (1992) The role of indole-3–acetic acid in mediating changes in stem elongation of etiolated Pisum seedlings following exposure to light. In: Karssen CM, van Loon LC and Vreugdenhil D (eds) Progress in Plant Growth Regulation. Kluwer Academic Publishers, pp 437–445
Bialek K, Meudt WJ and Cohen JD (1983) Indole-3–acetic acid (IAA) and IAA conjugates applied to bean stem sections. Plant Physiol 73: 130–134
Catalá C, Östin A, Chamarro J, Sanberg G and Crozier A (1992) Metabolism of indole-3–acetic acid by pericarp discs from immature and mature tomato (Lycopersicon esculentum Mill.). Plant Physiol 100: 1457–1463
Cohen JD and Bandurski RS (1978) The bound auxins: protection of indole-3–acetic acid from peroxidase-catalyzed oxidation. Planta 139: 203–208
Davies PJ (1976) Bound auxin formation in growing stems. Plant Physiol 57: 197–202
Delbarre A, Muller P, Imhoff V, Morgat JL and Barbier-Brygoo H (1994) Uptake, accumulation and metabolism of auxins in tobacco leaf protoplasts. Planta 195: 159–167
Ehmann A (1977) The Van Urk-Salkowski reagent-a sensitive and specific chromogenic reagent for silica gel thinlayer chromatographic detection and identification of indole derivatives. J Chromatogr 132: 267–276
Hoad GV (1995) Transport of hormones in the phloem of higher plants. Plant Growth Regul 16: 173–182
Jakas A, Magnus V, Horvat S and Sandberg G (1993) Synthesis of β-D-glucosyl ester of (carboxy-13C)-indole-3–acetic acid. J Labelled Compd and Radiopharm 33: 933–939
Jakubowska A, Kowalczyk S and Leznicki AJ (1993) Enzymatic hydrolysis of 4–O-and 6–O-indol-3–ylacetyl-β-D-glucose in plant tissues. J Plant Physiol 142: 61–66
Kesy JM and Bandurski RS (1990) Partial purification and characterization of indol-3–ylacetylglucose:myo-inositol indol-3–ylacetyltransferase (indoleacetic acid-inositol synthase). Plant Physiol 94: 1598–1604
Knop W (1861) Quantitätive analytische Arbeiten über den Ernährungsprocess der Pflanzen. Landw Versuchsstat 3: 295–324
Komoszynski M and Bandurski RS (1986) Transport and metabolism of indole-3–acetyl-myo-inositol-galactoside in seedlings of Zea mays. Plant Physiol 80: 961–964
Kowalczyk S and Bandurski RS (1990) Isomerization of 1–Oindol-3–ylacetyl-β-D-glucose. Enzymatic hydrolysis of 1–O, 4–O, and 6–O-indol-3–ylacetyl-β-D-glucose and the enzymatic synthesis of indole-3–acetyl glycerol by a hormone metabolizing complex. Plant Physiol 94: 4–12
Law DM and Davies PJ (1990) Comparative indole-3–acetic acid levels in the slender pea and other pea phenotypes. Plant Physiol 93: 1539–1543
Leznicki AJ and Bandurski RS (1988) Enzymic synthesis of indole-3–acetyl-1–O-β-D-glucose. II. Metabolic characteristics of the enzyme. Plant Physiol 88: 1481–1485
Ludwig-Müller J, Epstein E and Hilgenberg W (1996) Auxinconjugate hydrolysis in Chinese cabbage: Characterization of an aminohydrolase and its role during infection with clubroot disease. Physiol Plant 97: 627–634
McKay MJ, Ross JJ, Lowrence NL, Cramp RE, Beveridge CA and Reid JB (1994) Control of internode length in Pisum sativum. Further evidence for the involvement of indole-3–acetic acid. Plant Physiol 106: 1521–1526
Monteiro AM, Crozier A and Sandberg G (1988). The biosynthesis and conjugation of indole-3–acetic acid in germinating seed and seedlings of Delbergia dolichopetala. Planta 174: 561–568
Nordström AC and Eliasson L (1991). Levels of endogenous indole-3–acetic acid and indole-3–acetylaspartic acid during adventitious root formation in pea cuttings. Physiol Plant 82: 599–605
Nowacki J and Bandurski RS (1980) Myo-inositol esters of indole-3–acetic acid as seed auxin precursors of Zea mays L. Plant Physiol 65: 422–427
Nowacki J, Cohen JD and Bandurski RS (1978) Synthesis of 14C-indole-3–acetyl-myo-inositol. J Labelled Compd Radiopharm 15: 325–329
Paliyath G, Rajagopal I, Unnikrishnan PO and Mahadevan S (1989). Hormones and Cuscuta development: IAA uptake, transport and metabolism in relation to growth in the absence and presence of applied cytokinin. J Plant Growth Regul 8: 19–35
Sasaki K, Sakai S, Kamada H and Harada H (1994) Identification of conjugated IAA in carrot crown gall as indole-3–acetylaspartic acid (IAAsp) by LC/MS. J Plant Growth Regul 13: 183–186
Slovin JP and Cohen JD (1993) Auxin metabolism in relation to fruit ripening. Acta Horticulturae (Plant Growth Regulators) 329: 84–89
Szerszen JB, Szczyglowski K and Bandurski RS (1994) iaglu, a gene from Zea mays involved in conjugation of the growth hormone, indole-3–acetic acid (IAA). Science 265: 1699–1701
Tsurumi S and Wada S (1990) Oxidation of indole-3–acetylaspartic acid in Vicia. In: Pharis RS and Rood SB (eds) Plant Growth Substances 1988. Springer-Verlag, pp 353–359
Tuominen H, Östin A, Sandberg G and Sundberg B (1994) A novel metabolic pathway for indole-3–acetic acid in apical shoots of Populus tremula (L.) × Populus tremuloides (Michx.). Plant Physiol 106: 1511–1520
Venis MA (1972) Auxin-induced conjugation systems in peas. Plant Physiol 49: 24–27
Yahalom A, Epel BL and Glinka Z (1988) Photomodulation of mesocotyl elongation in maize seedlings: Is there a correlative relationship between phytochrome, auxin and cell wall extensibility? Physiol Plant 72: 428–433
Zelená E, Kutáček M and Čermák V (1988) Fate of root applied indolylacetic acid and its influence on the growth of intact plants. In: Kutáček M, Bandurski RS and Krekule J (eds) Physiology and Biochemistry of Auxins in Plants. Academia Praha, pp 371–376
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Zelená, E. The effect of light on metabolism of IAA in maize seedlings. Plant Growth Regulation 30, 23–29 (2000). https://doi.org/10.1023/A:1006307519028
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DOI: https://doi.org/10.1023/A:1006307519028