Abstract
A PCR assay for the detection of small ruminant lentiviral gag DNA (provirus) in the white blood cells of sheep and goats was developed and compared with a serological test (AGIDT). A sample of the DNA prepared from the white blood cells in 3 ml of blood from 208 sheep and goats from 18 different flocks was subjected to PCR assay. One of 85 animals from flocks accredited under the Dutch national MVV/CAEV control programme was positive by PCR while none was positive by AGIDT. In infected flocks, the AGIDT appeared slightly more sensitive, but preliminary results show that the sensitivity of the PCR assay may be further improved by increasing the number of monocytes tested. The PCR assay, however, was clearly more sensitive in detecting animals in the early stages of infection. With the use of a set of mixed primers and probes, the assay was able to detect the variety of CAEV and MVV strains occurring in the field.
Similar content being viewed by others
REFERENCES
Barlough, J., East, N., Rowe, J.D., van Hoosear, K., DeRock, E., Bigornia, L. and Rimstad, E., 1994. Double-nested polymerase chain reaction for detection of caprine arthritis-encephalitis virus proviral DNA in blood, milk, and tissues of infected goats. Journal of Virological Methods, 50, 101-114.
Brodie, S.J., Pearson, L.D., Snowder, G.D. and DeMartini, J.C., 1992. Host-virus interaction as defined by amplification of viral DNA and serology in lentivirus-infected sheep. Archives of Virology, 130, 413-428
Dawson, M., Biront, P. and Houwers, D.J., 1982. Comparison of serological tests used in three state veterinary laboratories to identify maedi-visna virus infection. Veterinary Record, 111, 432-434
Haase, A.T., Retzel, E.F. and Staskus, K.A., 1990. Amplification and detection of lentiviral DNA inside cells. Proceedings of the National Academy of Sciences of the USA, 87, 4971-4975
Houwers, D.J. and Schaake, J., Jr, 1987. An improved ELISA for the detection of antibodies to ovine and caprine lentiviruses, employing monoclonal antibodies in a one-step assay. Journal of Immunological Methods, 98, 151-154
Houwers, D.J., König, C.D.W., Bakker, J., de Boer, M.J., Pekelder, J.J., Sol, J., Vellema, P. and de Vries, G., 1987. Maedi-visna control in sheep. III. Results and evaluation of a voluntary control program in the Netherlands over a period of four years. Veterinary Quarterly, 9, 29S-36S
Johnson, L.K., Meyer, A.L. and Zink, M.C., 1992. Detection of ovine lentivirus in seronegative sheep by in situ hybridization, PCR, and cocultivation with susceptible cells. Clinical Immunology and Immunopathology, 65, 254-260
Leroux, C., Lerondelle, C., Chastang, J. and Mornex, J.F., 1997. RT-PCR detection of lentiviruses in milk or mammary secretions of sheep or goats from infected flocks. Veterinary Research, 28, 115-121
Pekelder, J.J., Veenink, G.J., Akkermans, J.P.W.M., van Eldik, P., Elving, L. and Houwers, D.J., 1994. Ovine lentivirus induced indurative lymphocytic mastitis and its effect on the growth of lambs. Veterinary Record, 134, 348-350
Reddy, P.G., Sapp, W.J. and Heneine, W., 1993. Detection of caprine arthritis-encephalitis virus by polymerase chain reaction. Journal of Clinical Microbiology, 31, 3042-3043
Russo, P., Vitu, C., Bourgogne, A., Vignoni, M., Abadie, G., David, V. and Pepin, M., 1997. Caprine arthritis-encephalitis virus: detection of proviral DNA in lactoserum cells. Veterinary Record, 140, 483- 484
Zanoni, R.G., Nauta, I.M., Pauli, U. and Peterhans, E., 1991. Expression of E. coli and sequencing of the coding region for the capsid protein of Dutch maedi-visna strain ZZV 1050: application of the recombinant protein in enzyme-linked immunosorbent assay for the detection of caprine and ovine lentiviruses. Journal of Clinical Microbiology, 29, 1290-1294
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wagter, L., Jansen, A., Bleumink-Pluym, N. et al. PCR detection of lentiviral GAG segment DNA in the white blood cells of sheep and goats. Vet Res Commun 22, 355–362 (1998). https://doi.org/10.1023/A:1006181307002
Issue Date:
DOI: https://doi.org/10.1023/A:1006181307002