Abstract
The greatest number of embryos was obtained using young carrot callus cultured on Gamborg's B-5 medium containing 1 g l-1 casein hydrolysate and 10 -10 M 2,4-dichlorophenoxyacetic acid. The largest increase in embryogenesis for old cultures also was obtained using Gamborg's B-5 medium supplemented with 1 g l-1 casein hydrolysate and 10 -10 M 2,4-dichlorophenoxyacetic acid; however, no combination of factors for the older culture restored the initial vigorous morphogenetic response seen in young cultures.
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References
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Smith, R.A., Jacobs, D., Desai, D. et al. Optimization of culture medium extends response time of embryogenic carrot cells but does not restore initial high response of young cultures. Plant Cell, Tissue and Organ Culture 49, 63–65 (1997). https://doi.org/10.1023/A:1005893814507
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DOI: https://doi.org/10.1023/A:1005893814507