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Colorimetric method for the determination of ethanol by Flow Injection Analysis

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Abstract

An automated Flow Injection Analysis system using stop-flow technique for quantifying ethanol based in a colorimetric detection method was developed. The system permitted analysis in a linear range of 0.05–1 g ethanol l−1 without external dilution, a sampling frequency of 15 analyses per hour, and a relative standard deviation of 3.5%. A dilution line was implemented in the FIA system permitting the extension of the linear range to 0.5–5.2 g ethanol l−1 maintaining the same sampling frequency and standard deviation. The system was applied to measure ethanol concentrations present in samples of an alcoholic fermentation and the results showed no significant difference with other analytical procedures (GC).

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References

  • Lazaro F, Luque de Castro MD, Valcarcel M (1986) Sequential determination of glucose and fructose in foods by flow injection analysis with immobilized enzymes. Anal. Chim. Acta 340: 143–148.

    Google Scholar 

  • Ruzicka J, Hansen EH, Rietz B (1975) Flow Injection Analysis – a survey of its potential for continuous monitoring of industrial process. Anal. Chim. Acta 89: 241–246.

    Google Scholar 

  • Salgado AM, Folly ROM, Valdman B, Valero F (1998) Development of a sucrose enzymatic biosensor. Biotechnol. Tech. 12: 305–307.

    Google Scholar 

  • Tarnowski DJ, Korzeniewski C (1996) Amperometric detection with membrane-based sampling for percent level determination of ethanol. Anal. Chim. Acta 322: 111–121.

    Google Scholar 

  • Valero F, Lafuente J, Poch M, Solá C, Araujo AN, Lima JLFC (1990) On-line fermentation monitoring using flow injection analysis. Biotechnol. Bioeng. 36: 647–651.

    Google Scholar 

  • Worsfold PJ, Ruzicka J, Hansen EH (1981) Rapid automated enzymatic method determination of alcohol in blood and beverages using flow injection analysis. Analyst 106: 1308–1317.

    Google Scholar 

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Authors and Affiliations

  1. Universitat Autonoma de Barcelona, 08193, Bellaterra, Spain

    A.M. Salgado, R.O.M. Folly, O. Cos & F. Valero

  2. Departamento de Engenharia Química – Escola de Química –, Universidade Federal do Rio de Janeiro, Rio de Janeiro C.T., B1 E sl 211, Brasil

    B. Valdman

  3. Departamento d'Enginyeria Química, ETSE, Brasil

    B. Valdman

Authors
  1. A.M. Salgado
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  2. R.O.M. Folly
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  3. B. Valdman
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  4. O. Cos
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  5. F. Valero
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Salgado, A., Folly, R., Valdman, B. et al. Colorimetric method for the determination of ethanol by Flow Injection Analysis. Biotechnology Letters 22, 327–330 (2000). https://doi.org/10.1023/A:1005651205723

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  • DOI: https://doi.org/10.1023/A:1005651205723

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