Abstract
When growth-phase cell suspension cultures of Scutellaria baicalensis were treated with 50 μg of yeast elicitor preparation ml−1, both oleanolic acid and ursolic acid transiently increased in the culture medium rather than in the cells. The maximal triterpenoid concentration was 13.7 mg l−1 media approx. 35 h after treatment, whereas the maximum concentration was 2.1 mg l−1 media after about 20 h following treatment with methyl jasmonate. Elicitor treatment also doubled phospholipase A2 activity (25 pmol mg−1 min) and the simultaneous treatment of aristolochic acid, a phospholipase A2 inhibitor, inhibited triterpenoids accumulation as well as phospholipase A2 activity.
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Yoon, H.J., Kim, H.K., Ma, CJ. et al. Induced accumulation of triterpenoids in Scutellaria baicalensis suspension cultures using a yeast elicitor. Biotechnology Letters 22, 1071–1075 (2000). https://doi.org/10.1023/A:1005610400511
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DOI: https://doi.org/10.1023/A:1005610400511