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Construction and characterization of a linked-dimeric pyrroloquinoline quinone glucose dehydrogenase

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Abstract

Using the structural gene of a homo dimeric enzyme, the water-soluble pyrroloquinoline quinone glucose dehydrogenase (PQQGDH-B), a gene consisting of two identical subunits linked together by a DNA segment coding linker peptide region was constructed. Using the constructed gene, a linked-dimeric PQQGDH-B was produced in Escherichia coli as the active soluble enzyme. Linked-dimeric PQQGDH-B showed a larger increase in thermal stability than the native dimeric enzyme. During incubation over 45 °C, the residual activity of linked-dimeric PQQGDH-B was more than twice that of the native dimeric enzyme. The potential application of linked-dimeric PQQGDH-B for glucose enzyme sensor is also discussed.

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Authors and Affiliations

  1. Department of Biotechnology, Tokyo University of Agriculture & Technology, 2-24-16 Nakamachi, Koganei, Tokyo, 184-8588, Japan

    Koji Sode, Miki Shirahane & Hiromi Yoshida

Authors
  1. Koji Sode
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  2. Miki Shirahane
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  3. Hiromi Yoshida
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Sode, K., Shirahane, M. & Yoshida, H. Construction and characterization of a linked-dimeric pyrroloquinoline quinone glucose dehydrogenase. Biotechnology Letters 21, 707–710 (1999). https://doi.org/10.1023/A:1005518610946

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  • DOI: https://doi.org/10.1023/A:1005518610946

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