Abstract
Brassica nigra, a weed and minor crop species, may be a source of useful traits for breeders of major Brassica crops. However, little is known about the extent and distribution of genetic variation in B. nigra, and few markers have been developed to describe this variation. In an initial survey of variation in B. nigra, we used five amplified DNA fragment markers based on SSRs to describe variation in 32 entries (genebank accessions and weed populations) from four regions: Europe/North Africa (the species' native range), India, Ethiopia, and North America. Considerable variation was apparent between regions and between entries, but more than half of the total variation was between plants within entries. Ethiopian entries formed the most distinct group. European and North American entries comprised the nost variation and were generally grouped together. Unique variants were present in weed populations from North America (where the species is introduced), but marker variation between the populations was not correlated with geographic distance. In summary, SSR-based markers were informative descriptors of variation in a global sampling of B. nigra germplasm. The patterns of variation appear to be consistent with the species' agricultural history, and they can be used to design further studies and plan strategies for conserving B. nigra genetic resources.
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Westman, A.L., Kresovich, S. Simple sequence repeat (SSR)-based marker variation in Brassica nigra genebank accessions and weed populations. Euphytica 109, 85–92 (1999). https://doi.org/10.1023/A:1003637814963
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DOI: https://doi.org/10.1023/A:1003637814963