Abstract
Environmental effects on genetic variation for chilling resistance were studied in nine cultivars and breeding lines (referred to as cultigens hereafter) of cucumber (Cucumis sativus L.). Five experiments were carried out in controlled-environment chambers to measure the effects of growth temperature, photoperiod, duration of chilling, light level during chilling, and watering frequency on chilling resistance of seedlings at the cotyledon and first true leaf growth stages. Significant interactions were found between cultigen and all environmental factors studied except for the photoperiod and watering frequency. Cultigen rank was affected by growth temperature before chilling, chilling duration, and light level during chilling, but shifts in rank were not consistent. Genetic variation was largest when the plants were grown at 22/18 °C, most pronounced after a chilling duration of 5 to 9 hours and a light level during chilling of 270 µmol·m-2·s-1. Variation was larger at the first true leaf stage than at the cotyledon stage. Differences among cultigens in chilling damage were largest 5 days after chilling. Therefore, it seems that testing for genetic variation in chilling damage can be restricted to one set of environmental conditions. We recommend the following conditions for screening cucumber for genetic variation in chilling resistance: grow the plants at 22/18 °C, under a 9-hour photoperiod with a 3-hour night interruption, water them once daily, subject them at the first true leaf stage to a chilling treatment of 7 hours at 4°C at a light level of 270 µmol·-2·s-1, and evaluate damage 5 days after treatment.
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References
Aoki, S., M. Oda & K. Hosino, 1989. Varietal differences in chilling-induced depression of photosynthesis and leaf growth in cucumber seedlings. J Japan Soc Hort Sci 58: 173–179.
Aoki, S., M. Oda & M. Nagaoka, 1988. Chilling and heat sensitivities in cucumber seedlings measured by chlorophyll fluorescence. Bull Nat Res Inst Veg, Ornam Plants & Tea Japan. Ser. A, No. 2: 81–92.
Cabrera, R.M., M.E. Saltveit & K. Owens, 1992. Cucumber cultivars differ in their response to chilling temperatures. J Amer Soc Hort Sci 117: 802–807.
Downs, R.J. & J.F. Thomas, 1991. Phytotron Procedural Manual for Controlled-Environment Research at the Southeastern Plant Environment Laboratory. North Carolina Agricultural Research Service Tech Bul 244 (revised), 43 pp.
Hughes, G.R., C.W. Averre & K.A. Sorensen, 1983. Growing pickling cucumbers in North Carolina. N.C. Agric. Ext Serv Bulletin No. AG-315, 15 pp.
Lafuente, M.T., A. Belver, M.G. Guye & M.E. Saltveit, 1991. Effect of temperature conditioning on chilling injury of cucumber cotyledons. Possible role of abscisic acid and heat shock proteins. Plant Physiol 95: 443–449.
Lasley, S.E., M.P. Garber & C.F. Hodges, 1979. Aftereffects of light and chilling temperatures on photosynthesis in excised cucumber cotyledons. J Amer Soc Hort Sci 104: 477–480.
Liu, H.X., Y.R. Wang, S.X. Zeng, P. Li, Y.Z. Chen, D.F. Chen & J.Y. Guo (C.Y. Kuo), 1984. The effect of chilling stress on respiratory metabolism in cucumber seedlings with different degrees of tolerance. Acta Phytophysiol Sin 10: 191–199.
Lower, R.L., 1974. Measurement and selection for cold tolerance in cucumber. Pickle Pak Sci 4: 8–11.
Minchin, A. & E.W. Simon, 1973. Chilling injury in cucumber leaves in relation to temperature. J Exp Bot 24: 1231–1235.
Nienhuis, J., R.L. Lower & J.E. Staub, 1983. Selection for improved low temperature germination in cucumber. J Amer Soc Hort Sci 108: 1040–1043.
Pomeroy, M.K. & J.B. Mudd, 1987. Chilling sensitivity of cucumber cotyledon protoplasts and seedlings. Plant Physiol 84: 677–681.
Rietze, E., 1988. Wirkungen kurzfristiger Kälteperioden auf Gurken (Cucumis sativus L.). Ph.D. thesis, University of Hannover, Germany, p.90.
Rietze, E. & H.J. Wiebe, 1987. Kälte im Licht mögen Pflanzen nicht. Deutscher Gartenbau 41: 2436–2437.
Rietze, E. & H.J. Wiebe, 1989. Diurnal rhythm of chilling sensitivity of cucumbers in light. Sci Hortic 38: 231–237.
Rikin, A., A. Blumenfeld & A.E. Richmond, 1976. Chilling resistance as affected by stressing environments and abscisic acid. Bot Gaz 137: 307–312.
Saczynska, V., J. Gemel & Z. Kaniuga, 1993. Chilling susceptibility of Cucumis species. Phytochemistry 33: 61–67.
Saltveit, M.E., 1991. Prior temperature exposure affects subsequent chilling sensitivity. Physiol Plant 82: 529–536.
SAS/STAT User's Guide, Release 6.03 edition, 1988. SAS Institute, Cary, NC, USA.
Soule, J., 1985. Glossary for horticultural crops. John Wiley & Sons, New York, 308 pp.
Staub, J.E., 1988. Lack of chilling resistance in Cucumis sativus var. hardwickii (R.) Alef. Cucurbit Genet Coop Rpt 11: 29–32.
Staub, J.E., R.L. Lower & J. Nienhuis, 1988. Correlated responses to selection for low temperature germination in cucumber. HortScience 23: 745–746.
Tánczos, O.G., 1974. Invloed van lage temperatuur op de bladeren van Cucumis sativus L. Ph.D. thesis, University of Groningen, The Netherlands, 89 pp.
United States Department of Agriculture. Agricultural Statistics. 1993, p 146.
Van Hasselt, P.R., 1972. Photo-oxidation of leaf pigments in Cucumis leaf discs during chilling. Acta Bot Neerl 21: 539–548.
Wang, C.Y., 1986. Effects of temperature and light on ACC and MACC in chilled cucumber seedlings. Sci Hortic 30: 47–52.
Wehner, T.C., 1981. Screening for low-temperature germination ability in cucumber. HortScience 16: 399 (Abstr.).
Wehner, T.C., 1982. Genetic variation for low-temperature germination ability in cucumber. Cucurbit Genet Coop Rpt 5: 16–17.
Wehner, T.C., 1984. Estimates of heritabilities and variance components for low-temperature germination ability in cucumber. J Amer Soc Hort Sci 109: 664–667.
Wehner, T.C., J.E. Staub & J.P. Palta, 1997. Tolerance to low temperatures in cucumber. HortScience (in press).
Wilson, J.M. & R.M.M. Crawford, 1974. Leaf fatty-acid content in relation to hardening and chilling injury. J Exp Bot 25: 121–131.
Wright, M. & E.W. Wilson, 1973. Chilling injury in cucumber leaves. J Exp Bot 24: 400–411.
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Smeets, L., Wehner, T.C. Environmental effects on genetic variation of chilling resistance in cucumber. Euphytica 97, 217–225 (1997). https://doi.org/10.1023/A:1003084821178
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DOI: https://doi.org/10.1023/A:1003084821178