biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 39:33-41, 1997 | DOI: 10.1023/A:1000988316206

Transient b-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens

S. Rakouský1, T. Kocábek2, R. Vincenciová2, M. Ondřej1
1 Academy of Sciences of the Czech Republic, Ćeské, Institute of Plant Molecular Biology, Budějovice, CZ -, Czech Republic
2 Faculty of Biological Sciences, University of South Bohemia, Ćeské, Budějovice, CZ -, Czech Republic

Transient expression of the β-glucuronidase (GUS) gene introduced into Arabidopsis thaliana intact plants by T-DNA after vacuum infiltration of Agrobacterium tumefaciens was followed. The first incidence of GUS activity was found 2 - 3 d after treatment and a peak of activity one week after treatment in both A. thaliana races, Columbia and C24. GUS activity was sharply increased by cultivation of Arabidopsis plants at elevated temperature (29 °C) compared to cultivation at 25 °C. The density of inocula also influenced the GUS activity.

Keywords: T-DNA; temperature effect; inoculum density
Subjects: Agrobacterium tumefaciens infiltration; Arabidopsis thaliana; b-glucuronidase expression, Agrobacterium infiltration, temperature; inoculum density, Agrobacterium, b-glucuroni-dase expression

Published: July 1, 1997  Show citation

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Rakouský, S., Kocábek, T., Vincenciová, R., & Ondřej, M. (1997). Transient b-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens. Biologia plantarum, 39(1), 33-41. doi: 10.1023/A:1000988316206
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