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Isolation and Properties of Major Components of Penicillium canescens Extracellular Enzyme Complex

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Abstract

The composition of the enzyme complex secreted by Penicillium canescens was investigated. A scheme for purification of the main components of the complex by chromatofocusing on a Mono P column was developed. It was found that along with β-galactosidase, the major components of the complex were endo-β-1,4-xylanase (31 kD, pI 8.2-9.3), α-L-arabinofuranosidase (60 kD, pI 7.6), arabinoxylan-arabinofuranohydrolase (70 kD, pI 3.8-4.0), and endo-β-1,3/1,4-glucanase (40 kD, pI 4.4). The substrate specificity, pH and temperature activity optima, adsorbability, thermal stability, and ability for synergic interaction of the isolated enzymes were studied.

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Authors and Affiliations

  1. Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119992, Russia

    O. A. Sinitsyna, F. E. Bukhtoyarov, A. V. Gusakov & A. P. Sinitsyn

  2. Russian Academy of Sciences, Institute of Biochemistry and Physiology of Microorganisms, Pushchino, 142292, Moscow Region, Russia

    O. N. Okunev & A. O. Bekkarevitch

  3. Institute of Genetics and Selection of Industrial Microorganisms, I Dorozhnyi Proezd 1, Moscow, 113545, Russia

    Yu. P. Vinetsky

Authors
  1. O. A. Sinitsyna
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  2. F. E. Bukhtoyarov
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  3. A. V. Gusakov
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  4. O. N. Okunev
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  5. A. O. Bekkarevitch
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  6. Yu. P. Vinetsky
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  7. A. P. Sinitsyn
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Sinitsyna, O.A., Bukhtoyarov, F.E., Gusakov, A.V. et al. Isolation and Properties of Major Components of Penicillium canescens Extracellular Enzyme Complex. Biochemistry (Moscow) 68, 1200–1209 (2003). https://doi.org/10.1023/B:BIRY.0000009134.48246.7e

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  • DOI: https://doi.org/10.1023/B:BIRY.0000009134.48246.7e

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