Abstract
Genetic relationships among 18 accessions, including 16 of Ananas and two of Pseudananas, were investigated using RAPD molecular markers. The procedure for DNA extraction was adapted from the method of Dellaporta et al. (1983) where an incubation in proteinase K and a purification step were included. From the total of 148 markers scored,132 (89.2%) were polymorphic. The similarity matrix was used for cluster analysis. The phenogram developed from the RAPD bands showed that for most of the cases, the accessions within a species grouped together. Nevertheless, a moderate infraspecific genetic variation was observed. For example, DNA data grouped all A. comosus accessions with a mean similarity coefficient of 0.85. Comparable results were obtained with all other species investigated. The highest genetic divergence was found withinA. lucidus where the mean similarity coefficient among accessions was0.75. A similar level of genetic polymorphism was observed among species,therefore, a definition about which species were involved in the constitution of A. comosus genotypes was not possible. These results agree with the breeders standpoint suggesting that all Ananas species belong to the primary gene pool of pineapple.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aradhya, M.K., F. Zee & R.M. Manshardt, 1994. Isozyme variation in cultivated and wild pineapple. Euphytica 79: 87–99.
Cabbot, C.L., 1990. Etat des connaissances botaniques, cytogenétiques et biologiques sur la reproduction de l'ananas. Fruits 45: 347–355.
Cabbot, C.L., 1992. Origin, phylogeny and evolution of pineapple species. Fruits 47: 25–32.
Collins, J.L., 1960. The pineapple. Botany, cultivation, and utilization. Leonard Hill Limited, London, 294 pp.
Collins, J.L. & K.R. Kerns, 1946. Inheritance of the leaf types in the Pineapple. J Hered 37: 123–127.
Dellaporta, S.L., J. Wood & J.B. Hicks, 1983. A plant DNA minipreparation: Version II. Pl Mol Biol Rep 1: 19–21.
DeWald, M.G., G.A. Moore & W.B. Sherman, 1992. Isozymes in Ananas (Pineapple): Genetics and Usefulness in Taxonomy. J Amer Soc Hort 117: 491–496.
Dujardin, M., 1991. Cytogenetique de l'ananas. Fruits (numéro spécial Ananas): 376–278.
Duval, M.F. & G.C. D'Eeckenbrugge, 1993. Genetic variability in the genus Ananas. ActaHortic 334: 27–32.
Duval, M.F. & G.C. D'Eeckenbrugge, F.R. Ferreira, J.R.S. Cabral & L.B. Bianchetti, 1997. First results from joint Embrapa-Cirad Ananas germplasm collection in Brazil and French Guyana. Acta Hortic 425: 137–144.
Evain, D., 1988. Nouvelles donnees sur l'incompatibilité chez le genre Ananas. DAA ENSA Toulouse, 43 pp.
Ferreira, F.R., D.C. Giacometti, L.B. Bianchetti & J.R.S. Cabral, 1992. Coleta de germoplasma de abacaxizeiros (Ananas comosus (L.) Merrill). Rev Bras Frutic 14: 5–11.
Ferreira, F.R. & J.R.S. Cabral, 1993. Pineapple germplasm in Brazil. Acta Hort 334: 23–26.
Ferreira, F.R., J.R.S. Cabral & M.F. Duval, 1995. Occurrence and distribution of Ananas and Pseudananas in Brazil. 2nd Symposium International of Ananas. Trois - Ilets. Martinique.
Garcia, M.L., 1988. Etude taxonomique du genre Ananas. Utilization de la variabilité enzymatique. Thèse UST Languedoc, Montpellier, France. 152 pp.
Leal, F., 1989. On the history, origin and taxonomy of the pineapple. Interciencia 14: 235–241.
Leal, F. & M.G. Antoni, 1980a. Espécies del genero Ananas: origen y distributión geográfica. Rev Fac Agron (Maracay), Alcance 29: 5–12.
Leal, F. & M.G. Antoni, 1980b. Sobre las espécies del genero Ananas: origen y distributión especialmente novedora para Venezuela. Rev Fac Agron (Maracay), Alcance 29: 43–50.
Leal, F. & L. Amaya, 1991. The Curaua (Ananas lucidus, Bromeliaceae) crop in Venezuela. Econ Botany 45: 216–224.
Leal, F. & G.C. D'Eeckenbrugge, 1996. Pineapple. In: J. Janick & J.N. Moore (Eds.), Fruit Breeding, Volume I: Tree and Tropical Fruits, pp. 515–557. John Wiley & Sons, Inc.
Lin, B.Y., P.S. Ritschell & F.R. Ferreira, 1987. NÚmero cromossômico de exemplares da família Bromeliaceae. Rev Bras Frutic 9: 49–55.
Noyer, J.L., 1991. Etude préliminaire de la diversité genétique du genre Ananas par les RFLPs. Fruits (numéro special Ananas): 372–375.
Noyer, J.L., C. Lanand, G.C. D'Eeckenbrugge & M.F. Duval, 1997. RFLP study of rDNA variability in Ananas Genus. Acta Horti 425: 153–160.
Py, C., J.J. Lacoeuilhe & C. Teisson, 1987. The pineapple: Cultivation and uses. G.P. Maisonneuve and Larose. 568 pp.
Reitz, P.R., 1968. A new species of pineapple from Central Brazil. The Bromeliad Soc Bull XYIII: 109–111.
Ruas, P.M., C.F. Ruas, D.J. Fairbanks, W.R. Andersen & J.R.S. Cabral, 1995. Genetic relationship among four varieties of pineapple, Ananas comosus, revealed by random amplified polymorphic DNA (RAPD) analysis. Brazilian J Genet 18: 413–416.
Smith, L.B. & R.J. Downs, 1979. Bromelioideae (Bromeliaceae). Flora Neotropica 14, part 3: 2048–2064.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
de Fátima Ruas, C., Ruas, P.M. & Cabral, J.R.S. Assessment of genetic relatedness of the genera Ananas and Pseudananas confirmed by RAPD markers. Euphytica 119, 245–252 (2001). https://doi.org/10.1023/A:1017597911205
Issue Date:
DOI: https://doi.org/10.1023/A:1017597911205