Abstract
A pilot-scale culture of multiple adventitious roots of ginseng was established using a balloon-type bubble bioreactor. Adventitious roots (2 cm) induced from callus were cultured in plastic Petri dishes having 20 ml of solid Schenk and Hildebrandt (1972) medium containing 3% sucrose, 0.15% gelrite, and 24.6 μM indole-3-butric acid. An average of 29 secondary multiple adventitious roots were produced after 4 weeks of culture. These secondary roots were elongated on the same medium, reaching a length of 5 cm after 6 weeks of culture. A time course study revealed that maximum yields in 5-l and 20-l bioreactors were approximately 500 g and 2.2 kg at day 42 with 60 g and 240 g inoculations, respectively. Cutting twice during the culture increased the total amount of biomass produced. The root biomass in a 20-l balloon-type bubble bioreactor was 2.8 kg at harvest with 240 g of inoculum after 8 weeks of culture. The total saponin content obtained from small-scale and pilot-scale balloon type bubble bioreactors was around 1% based on dry weight. Inoculation of 500 g fresh weight of multiple adventitious roots into a 500 l balloon-type bubble bioreactor with cutting at 4 and 6 weeks after inoculation produced approximately 74.8 kg of multiple roots. The ginsengnoside profiles of these multiple adventitious roots were similar to profiles of field-grown ginseng roots when analyzed by HPLC.
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References
Asaka I, Li L, Hirotani M, Asada Y & Furuya T (1993) Production of ginsenoside saponin by culturing ginseng (Panax ginseng) embryogenic tissues in bioreactors. Biotech Letters 15(12): 1259–1264
Akalezi CO, Liu S, Li QS, Yu JT & Zhong JJ (1997) Effect of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng. Proceedings of the Asia-Pacific biochemical engineering conference 97. Beijing, China. 20-23 October 1997 (pp 107–110)
Butenko RG (1964) Tissue culture and physiology of morphogenesis. In: Nauka M. (ed) (p 282)
Carvalho EB & Curtis WR (1998) Characterization of fluid flow resistance in root cultures with a convective flow tubular bioreactor. Biotechol. Bioeng. 60(3): 375–384
Curtis WR (1993) Cultivation of roots in bioreactors. Curr. Opinion Biotechnol. 4(2): 205–210
Furuya T, Yoshikawa T, Orihara Y & Oda H (1983) Saponin production in cell suspension cultures of Panax ginseng. Plant Med. 48: 83–87
Furuya T, Yoshikawa T, Orihara Y & Oda H (1984) Studies of the culture conditions for Panax ginseng cells in jar fermentor. J. Nat. Prod. 47: 70–75
Gamberg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 148–151
Ko KM, Song JJ, Hwang B & Kang YH (1993) Cytogenetic and historical characteristic of ginseng hairy root transformed by Agrobacterium rhizogenes. Korean J. Bot. 36(1): 75–81
Liu CZ, Wang YC, Onyang F, Ye HC & Li GF (1998) Production of artemisin by hairy root culture of Artemisia annua L. in bioreactor. Biotech. Letters 20(3): 265–268
Liu S & Zhong JJ (1998) Phosphate effect on of ginseng saponin and polysaccaride by cell suspension cultures of Panax ginseng and Panax quinquefolium. Process Biochem. 33(1): 69–74
Mathur A, Shukla YN, Pal M, Ahuja PS & Unuyal GC (1994) Saponin production in callus and cell suspension cultures of Panax quinquefolium. Phytochem. 35: 1221–1225
Murashige T & Skoog F (1962) Revised medium for rapid growth and bioassay with tobacco tissue culture. Phisiol. Plant 15: 473–479
Schenk RU & Hidebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50: 199–204
Son SH & Lee YH (1997) Automatic medium circulation type bioreactor for the cultivation of plant tissue and organ. Korea patent No. 97-37407
Yoshikawa T & Furuya T (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Rep. 6: 449–453
Yuan LH & Ouyang PK (1994) Studies on the improvement of cell growth and saponin production by cell cultures of Panax quinquefolium. J. Nanjing Ins. Chemical Tech. 16: 72–77
Zhong JJ, Bai Y & Wang DJ (1996) Effect of plant growth regulators on cell growth and ginsennoside saponin production by suspension cultures of Panax quinquefolium. J. Biotech. 45: 227–234
Zhong JJ & Wang SJ (1998) Effect of nitrogen source on the production of ginseng saponin and polysaccharide by cell culrues of Panax quinquefolium. Process Biochem. 33(6): 671–675
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Choi, S.M., Ho Son, S., Rho Yun, S. et al. Pilot-scale culture of adventitious roots of ginseng in a bioreactor system. Plant Cell, Tissue and Organ Culture 62, 187–193 (2000). https://doi.org/10.1023/A:1006412203197
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DOI: https://doi.org/10.1023/A:1006412203197