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Mapping minor QTL for increased stearic acid content in sunflower seed oil

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Abstract

Increased stearic acid (C18:0) content in the seed oil of sunflower would improve the oil quality for some edible uses. The sunflower line CAS-20 (C18:0 genotype Es1Es1es2es2), developed from the high C18:0 mutant line CAS-3 (C18:0 genotype es1es1es2es2; 25% C18:0), shows increased C18:0 levels in its seed oil (8.6%). The objective of this research was to map quantitative trait loci (QTL) conferring increased C18:0 content in CAS-20 in an F2 mapping population developed from crosses between HA-89 (wild type Es1Es1Es2Es2; low C18:0) and CAS-20, which segregates independently of the macromutation Es1 controlling high C18:0 content in CAS-3. Seed oil fatty acid composition was measured in the F2 population by gas-liquid chromatography. A genetic linkage map of 17 linkage groups (LGs) comprising 80 RFLP and 19 SSR marker loci from this population was used to identify QTL controlling fatty acid composition. Three QTL affecting C18:0 content were identified on LG3, LG11, and LG13, with all alleles for increased C18:0 content inherited from CAS-20. In total, these QTL explained 43.6% of the C18:0 phenotypic variation. Additionally, four candidate genes (two stearate desaturase genes, SAD6 and SAD17, and a FatA and a FatB thioesterase gene) were placed on the QTL map. On the basis of positional information, QTL on LG11 was suggested to be a SAD6 locus. The results presented show that increased C18:0 content in sunflower seed oil is not a simple trait, and the markers flanking these QTL constitute a powerful tool for plant breeding programs.

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Author notes

  1. Simon T. Berry

    Present address: Advanta Seeds UK Ltd., Station Road, Docking, King’s Lynn, Norfolk, PE31 8LS, UK

Authors and Affiliations

  1. Instituto de Agricultura Sostenible (CSIC), Alameda del Obispo s/n, Apartado 4084, E-14080, Córdoba, Spain

    Begoña Pérez-Vich & José M. Fernández-Martínez

  2. Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon, 97331, USA

    Steven J. Knapp

  3. Advanta Semillas, Ruta 226, Km 60.0, 7620, Balcarce, Buenos Aires, Argentina

    Alberto J. Leon

  4. Advanta Biotechnology Laboratory, SES-Europe NV/SA, B-3300, Tienen, Belgium

    Simon T. Berry

Authors
  1. Begoña Pérez-Vich
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  2. Steven J. Knapp
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  3. Alberto J. Leon
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  4. José M. Fernández-Martínez
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  5. Simon T. Berry
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Correspondence to Begoña Pérez-Vich.

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Pérez-Vich, B., Knapp, S.J., Leon, A.J. et al. Mapping minor QTL for increased stearic acid content in sunflower seed oil. Molecular Breeding 13, 313–322 (2004). https://doi.org/10.1023/B:MOLB.0000034081.40930.60

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  • DOI: https://doi.org/10.1023/B:MOLB.0000034081.40930.60

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