Abstract
Wild species of Oryza may serve as sources of superior drought tolerance alleles for cultivated rice. In a series of three screenhouse experiments, we compared traits associated with leaf water status, stomatal conductance, membrane stability, and root development in several wild Oryza accessions and O. sativa cultivars, when grown under well-watered or water-deficient conditions. One accession of O. longistaminata had greater stomatal conductance under stress and maintained leaf elongation better under stress than most other genotypes. Several upland-rice cultivars from the japonica subspecies also maintained greater conductance and leaf expansion than an indica cultivar or O. rufipogon accessions. Leaves of an accession of O. latifolia were particularly sensitive to water deficit. Oryza longistaminata and several O. rufipogon accessions had greater membrane stability, measured as electrolyte leakage from leaf disks after heat treatment, than cultivated rice. Drought treatments tended to reduce the levels of electrolyte leakage observed. Wild species had levels of osmotic adjustment under stress that were similar to O. sativa. Entries differed in the growth of roots with water deficit:O. longistaminata accessions and some japonica cultivars showed either an increase in total root mass or an increase in the proportion of root mass in deeper soil levels when water deficit was imposed, whereas indica cultivars and O. rufipogon accessions did not. Changes in root mass and distribution were correlated with values of stomatal conductance and leaf elongation; this suggests that leaf measurements made at key times in the drying cycle may reveal possible genetic differences in rooting behavior, which are otherwise difficult to measure. These studies indicate that O. longistaminata and O. rufipogon may serve as sources of novel alleles for improved leaf traits under drought stress–specifically, for maintenance of leaf elongation, stomatal conductance, and membrane stability. Alleles for improved root growth and distribution under water deficit exist in some japonica rice cultivars.
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References
Agarie, S., N. Hanaoka, F. Kubota, W. Agata & P.B. Kaufman, 1995. Measurement of cell membrane stability evaluated by electrolyte leakage as a drought and heat tolerance test in rice (Oryza sativa L.). J Faculty Agric Kyushu Univ 40: 233–240.
Ali, M.L., M.S. Pathan, J. Zhang, G. Bai, S. Sarkarung & H.T. Nguyen, 2000. Mapping QTLs for root traits in a recombinant inbred population from two indica ecotypes in rice. Theor Appl Genet 101: 756–766.
Babu, R.C., M.S. Pathan, A. Blum & T. Nguyen Henry, 1999. Com-parison of measurement methods of osmotic adjustment in rice cultivars. Crop Sci 39: 150–158.
Blum, A., J.X. Zhang & H.T. Nguyen, 1999. Consistent differences among wheat cultivars in osmotic adjustment and their relation-ship to plant production. Field Crops Res 64: 287–291.
Blum, A., N. Klueva & H.T. Nguyen, 2001. Wheat cellular ther-motolerance is related to yield under heat stress. Euphytica 117: 117–123.
Boonjung, H. & S. Fukai, 1996. Effects of soil water deficit at different growth stages on rice growth and yield under up-land conditions. 1. Growth during drought. Field Crops Res 48: 37–45.
Courtois, B., G. McLaren, P.K. Sinha, K. Prasad, R. Yadav & L. Shen, 2000. Mapping QTLs associated with drought avoidance in upland rice. Mol Breeding 6: 55–66.
De Datta, S.K., J.A. Malabuyoc & E.L. Aragon, 1988. Afield screen-ing technique for evaluating rice germplasm for drought tol-erance during the vegetative stage. Field Crops Res 19: 123–134.
Dingkuhn, M., M.P. Jones, D.E. Johnson & A. Sow, 1998. Growth and yield potential of Oryza sativa and O. glaberrima upland rice cultivars and their interspecific progenies. Field Crops Res 57: 57–69.
Fukai, S. & P. Inthapan, 1988. Growth and yield of rice culti-vars under sprinkler irrigation in south-eastern Queensland (Aus-tralia): 3. Water extraction and plant water relations: Compar-ison with maize and grain sorghum. Aust J Exp Agric 28: 249–252.
Fukai, S. & M. Cooper, 1995. Development of drought-resistant cul-tivars using physiomorphological traits in rice. Field Crops Res 40: 67–86.
IRRI, 1996. Standard Evaluation System for rice, INGER, Genetic Resource Center 4th Ed.
Jongdee, B., S. Fukai & M. Cooper, 2002. Leaf water potential and osmotic adjustment as physiological traits to improve drought tolerance in rice. Field Crops Res 76: 153–163.
Kamoshita, A., L.J. Wade, M.L. Ali, M.S. Pathan, J. Zhang, S. Sarkarung & H.T. Nguyen, 2002. Mapping QTLs for root mor-phology of a rice population adapted to rainfed lowland condi-tions. Theor Appl Genet 104: 880–893.
Lafitte, H. & J. Bennett, 2003. Requirements for aerobic rice: Physiological and molecular considerations. In: B. Bouman, et al. (Eds.), Water-Wise Rice Production. IRRI, Los Banos, Philippines.
Lafitte, H.R. & B. Courtois, 2002. Interpreting cultivar ×environ-ment interactions for yield in upland rice: Assigning value to drought-adaptive traits. Crop Sci 42: 1409–1420.
Mitchell, J.H., D. Siamhan, M.H. Wamala, J.B. Risimeri, E. Chinya-makobvu, S.A. Henderson & S. Fukai, 1998. The use of seedling leaf death score for evaluation of drought resistance of rice. Field Crops Res 55: 129–139.
Nguyen, H.T., R.C. Babu & A. Blum, 1997. Breeding for drought re-sistance in rice: Physiology and molecular genetic considerations. Crop Sci 37: 1426–1434.
O'Toole, J.C. & W.L. Bland, 1987. Genotypic variation in crop plant root systems. Adv Agron 41: 91–145.
Robin, S., M.S. Pathan, B. Courtois, H.R. Lafitte, C. Scarandang, S. Lanceras, M. Amante, H.T. Nguyen & Z. Li, 2003. Mapping osmotic adjustment in an advanced back-cross inbred population of rice. Theor Appl Genet 107: 1288–1296.
Sharp, R., Y. Wu, G. Voetberg, I. Saab & M. Lenoble, 1994. Con-firmation that abscisic acid accumulation is required for maize primary root elongation at low water potentials. J Exp Bot 45: 1743–1751.
Shen, L., B. Courtois, K.L. McNally, S. Robin & Z. Li, 2001. Evalua-tion of near-isogenic lines of rice introgressed with QTLs for root depth through marker-aided selection. Theor Appl Genet 103: 75–83.
Stiller, V., R. Lafitte & J. Sperry, 2003. Hydraulic properties of rice and the response of gas exchange to water stress. Plant Physiol 132: 1698–1706.
Tripathy, J.N., J. Zhang, S. Robin, T.T. Nguyen & H.T. Nguyen, 2000. QTLs for cell-membrane stability mapped in rice (Oryza sativa L.) under drought stress. Theor Appl Genet 100: 1197–1202.
Vaughan, D.A., 1994. The Wild Relatives of Rice IRRI, Los Baños, Philippines.
Xiao, J.H., J.M. Li, S. Grandillo, S.N. Ahn, L.P. Yuan, S.D. Tanksley & S.R. McCouch, 1998. Identification of trait-improving quanti-tative trait loci alleles from a wild rice relative, Oryza rufipogon. Genetics 150: 899–909.
Yoshida, S., D. Forno & J.H. Cock, 1976. Laboratory Manual for Physiological Studies of Rice, 3rd edn, IRRI, Los Baños, Philippines.
Zhang, J., H.G. Zheng, A. Aarti, G. Pantuwan, T.T. Nguyen, J.N. Tripathy, A.K. Sarial, S. Robin, R.C. Babu, B.D. Nguyen, S. Sarkarung, A. Blum & H.T. Nguyen, 2001. Locating genomic regions associated with components of drought resistance in rice: Comparative mapping within and across species. Theor Appl Genet 103: 19–29.
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Liu, L., Lafitte, R. & Guan, D. Wild Oryza species as potential sources of drought-adaptive traits. Euphytica 138, 149–161 (2004). https://doi.org/10.1023/B:EUPH.0000046801.27042.14
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DOI: https://doi.org/10.1023/B:EUPH.0000046801.27042.14