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Cloning and Sequencing of a 16 kDa Outer Membrane Protein Gene of Pasteurella multocida P52

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Abstract

The outer membrane proteins (OMPs) of Pasteurella multocida are potential immunogens. The 16 kDa OMP of P. multocida P52, serotype B:2, was identified as one of the major immunodominant antigens. The gene omp16, encoding a 16 kDa outer membrane protein, was amplified, cloned into a pBluescript SK(–) vector and sequenced. Complete sequence homology was observed between the 16 kDa OMP gene of P. multocida P52 (serotypes B:2) and P. multocida T16 (somatic serotype 3,4). This gene was distributed among different serotypes of P. multocida and found to localize in a 6.0 kb HindII fragment of the P. multocida genome.

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Authors and Affiliations

  1. National Biotechnology Centre, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, 243 12, U.P., India

    P.P. Goswami, P. Chaudhuri, V. Tiwari & N.S. Parihar

  2. Indian Veterinary Research Institute, Mukteshwar, Uttaranchal, India

    P.C. Harbola

Authors
  1. P.P. Goswami
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  2. P. Chaudhuri
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  3. V. Tiwari
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  4. N.S. Parihar
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  5. P.C. Harbola
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Goswami, P., Chaudhuri, P., Tiwari, V. et al. Cloning and Sequencing of a 16 kDa Outer Membrane Protein Gene of Pasteurella multocida P52. Vet Res Commun 28, 17–25 (2004). https://doi.org/10.1023/B:VERC.0000009529.26278.21

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  • DOI: https://doi.org/10.1023/B:VERC.0000009529.26278.21

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