Abstract
We investigated whether concentrations of carboxylates in the rhizosphere of chickpea (Cicer arietinum L.) roots were related to soil phosphorus levels. In a field experiment, cultivar Sona was grown at two P levels on eight soil types at three locations. There were large differences in extractable (0.2 mM CaCl2) rhizosphere carboxylate concentrations amongst the locations. The effect of P fertiliser was variable and carboxylate concentrations depended on soil type. To examine the effect of soil P in more detail, a glasshouse experiment was carried out, in which three cultivars (Heera, Sona and Tyson) were grown at four P levels on one soil type. The biomass of chickpea plants increased with increasing P level of the soil, and the root mass ratio decreased at the highest soil P level. However, rhizosphere concentrations of the carboxylates malonate, malate and citrate did not differ significantly between P treatments. This implied that there was no simple relation between available P and root exudation rates, in contrast to earlier results in studies using hydroponics. Cultivars differed in carboxylate concentration pattern: Sona and Tyson showed a tendency towards increased rhizosphere carboxylate concentrations at the second harvest, whereas the carboxylate concentration of Heera tended to decrease. It is hypothesised that chickpea roots always exude a basal level of carboxylates into the rhizosphere. They only increase carboxylate exudation considerably when the P availability is extremely low, which may occur in soils that strongly bind P.
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References
Allen D G and Jeffrey R C 1990 Methods for analysis of phosphorus in Western Australian soils. Report on investigation no. 37. Chemistry Centre of Western Australia, Perth, Australia.
Bolland M D A, Siddique K H M, Loss S P and Baker M J 1999 Comparing responses of grain legumes, wheat and canola to applications of superphosphate. Nutr. Cycl. Agroecosys. 53, 157–175.
Brouwer R 1983 Functional equilibrium: Sense or nonsense? Neth. J. Agr. Sci. 31, 335–348.
Colwell J D 1963 The estimation of phosphorus fertilizer requirements of wheat in southern New South Wales by soil analysis. Aust. J. Exp. Agric. Anim. Husb. 3, 190–198.
Dinkelaker B, Hengeler C and Marschner H 1995 Distribution and function of proteoid roots and other root clusters. Bot. Acta 108, 183–200.
Föhse D, Claassen N and Jungk A 1991 Phosphorus efficiency of plants. Plant Soil 132, 261–272.
Gardner W K, Barber D A and Parberry D G 1983 The acquisition of phosphorus by Lupinus albus L. III. The probable mechanism by which phosphorus movement in the soil/root interface is enhanced. Plant Soil 70, 107–124.
Gerke J 1994 Kinetics of soil phosphate desorption as affected by citric acid. Z. Pflanzenernähr. Bodenk. 157, 17–22.
Gerke J, Beißner L and Römer W 2000a The quantitative effect of chemical phosphate mobilization by carboxylate anions on P uptake by a single root. I. The basic concept and determination of soil parameters. J. Plant Nutr. Soil Sc. 163, 207–212.
Gerke J, Beißner L and Römer W 2000b The quantitative effect of chemical phosphate mobilization by carboxylate anions on P uptake by a single root. II. The importance of soil and plant parameters for uptake of mobilized P. J. Plant Nutr. Soil Sc. 163, 213–219.
Hoffland E, Findenegg G R and Nelemans J A 1989 Solubilization of rock phosphate by rape. II. Local root exudation of organic acids as a response to P-starvation. Plant Soil 113, 161–165.
Horst W J, Abdou M and Wiesler F 1993 Genotypic differences in phosphorus efficiency of wheat. In Plant nutrition-from genetic engineering to field practice. Ed. N J Barrow. pp. 367–370 Kluwer Academic Publishers, Dordrecht.
Jones D L 1998 Organic acids in the rhizosphere-a critical review. Plant Soil 205, 25–44.
Jones D and Farrar J 1999 Phosphorus mobilization by root exudates in the rhizosphere: Fact or fiction? Agrofor. Forum 9, 20–25.
Keerthisinghe G, Hocking P J, Ryan P R and Delhaize E 1998 Effect of phosphorus supply on the formation and function of proteoid roots of white lupin (Lupinus albus L.) Plant Cell Environ 21, 467–478.
Khan T N and Siddique K H M 2000 Registration of 'Heera' chickpea. Crop Sci. 40, 1501–1502.
Kitson R E and Mellon M G 1944 Colorimetric determination of phosphorus as molybdovanadophosphoric acid. Ind. Eng. Chem. Anal. Ed. 16, 379.
Lambers H, Cramer M D, Shane M W, Wouterlood M, Poot P and Veneklaas E J 2003 Structure and functioning of cluster roots and plant responses to phosphate deficiency. Plant Soil 248: 187–197.
Lazzaro M D and Thomson W W 1995 Seasonal variation in hydrochloric acid, malic acid, and calcium ions secreted by the trichomes of chickpea (Cicer arietinum). Physiol. Plant. 94, 291–297.
Li J and Copeland L 2000 Role of malonate in chickpeas. Phytochemistry 54, 585–589.
Marschner H 1995 Mineral nutrition of higher plants. Second edition. Academic Press, London.
Motomizu S, Wakimoto T and Toei K 1983 Spectrophotometric determination of phosphate in river waters with molybdate blue and malachite green. Analyst 108, 361–367.
Neumann G, Massonneau A, Langlade N, Dinkelaker B, Hengeler C, Römheld V and Martinoia E 2000 Physiological aspects of cluster root function and development in phosphorus-deficient white lupin (Lupinus albus L.). Ann. Bot. 85, 909–919.
Neumann G and Römheld V 1999 Root excretion of carboxylic acids and protons in phosphorus-deficient plants. Plant Soil 211, 121–130.
Ohwaki Y and Hirata H 1992 Differences in carboxylic acid exudation among phosphorus-starved leguminous crops in relation to carboxylic acid contents in plant tissues and phospholipid level in roots. Soil Sci. Plant Nutr. 38, 235–243.
Parfitt R L and Childs C W 1988 Estimation of forms of Fe and Al: A review, and analysis of contrasting soils by dissolution and Moessbauer methods. Aust. J. Soil Res. 26, 121–144.
Poorter H and Nagel O 2000 The role of biomass allocation in the growth response of plants to different levels of light, CO2, nutrients and water: A quantitative review. Aust. J. Plant Physiol. 27, 595–607.
Reuter D J 1997 Temperate and sub-tropical crops. In Plant analysis: an interpretation manual. Second edition. Eds. D J Reuter and J B Robinson pp. 83–284. CSIRO, Collingwood.
Shane M W, de Vos M, de Roock S and Lambers H 2003 Shoot phosphorus status regulates cluster-root growth and citrate exudation in Lupinus albus grown with a divided root system. Plant Cell Environ. 26: 265–273.
Siddique K H M, Brinsmead R B, Knight R, Knights E J, Paull J G and Rose I A 2000 Adaptation of chickpea (Cicer arietinum L.) and faba bean ( Vicia faba L.) to Australia. In Linking research and marketing opportunities for pulses in the 21st century. Ed. R Knight. pp. 289–303. Kluwer Academic Publishers, Dordrecht.
Siddique K H M and Khan T N 2000 Registration of 'Sona' chickpea. Crop Sci. 40, 1200.
Tang C, Fang R Y and Raphael C 1998 Factors affecting soil acidi-fication under legumes. II. Effect of phosphorus supply. Aust. J. Agr. Res. 49, 657–664.
Veneklaas E J, Stevens J, Cawthray G R, Turner S, Grigg A M and Lambers H 2003 Chickpea and white lupin rhizosphere carboxylates vary with soil properties and enhance phosphorus uptake. Plant Soil 248, 187–197.
Zhang F S, Ma J and Cao Y P 1997 Phosphorus deficiency enhances root exudation of low-molecular weight organic acids and utilization of sparingly soluble inorganic phosphates by radish (Raphanus sativus L.) and rape (Brassica napus L.) plants. Plant Soil 196, 261–264.
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Wouterlood, M., Cawthray, G.R., Turner, S. et al. Rhizosphere carboxylate concentrations of chickpea are affected by genotype and soil type. Plant and Soil 261, 1–10 (2004). https://doi.org/10.1023/B:PLSO.0000035568.28893.f6
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DOI: https://doi.org/10.1023/B:PLSO.0000035568.28893.f6