Skip to main content
SpringerLink
Log in

Inhibitory Effect of Aspergillus fumigatus Extract on matrix metalloproteinases Expression

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Background: Matrix metalloproteinases (MMPs) play a role in several physiologic and pathologic events. There are some evidence indicating the involvement of MMPs inthe pathophysiology of fungal infections. Methods: Here we study somatic extract of Aspergillus fumigatus. The influence of Aspergillus vs. two other fungal extracts on MMPs production by Fibrosarcoma cell line was investigated using an in vitro cytotoxicity assay, sodium dodecyl sulfate-polyacrylamide and gelatin zymography. Results: Comparative dose-dependent inhibitory effects on MMPs were seen by A. fumigatus extract and compared with some steroidal and non-steroidal drugs. Cytotoxicity analysis of our extract revealed much lower cell death than other examined agents. Conclusions: Since inhibition of MMPs activity has been employed in modality therapy in such diseases as cancer, this extract might be promising in the preparation of anti-MMP therapeutic derivatives.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nagase H, Woessner JF. Matrix metalloproteinases. J Biol Chem 1999; 274: 21491–21494.

    Google Scholar 

  2. Edwards, DR, Beaudry PP et al. The roles of tissue inhibitors of metalloproteinases in tissue remodelling and cell growth. Int J Obes 1996; 20: S9–S15.

    Google Scholar 

  3. Cunnane G, Fitzgerald O et al. Early joint erosions and serum levels of matrix metalloproteinase 1, matrix metalloproteinase 3 and tissue inhibitor of metalloproteinase 1 in rheumatoid arthritis. Arthritis Rheum 2001; 44(10): 2263–2274.

    Google Scholar 

  4. Katrib A, Tak PP et al. Expression of chemokines matrix metalloproteinases in early rheumatoid arthritis. Rheumatology (Oxford) 2001; 40(9): 988–994

    Google Scholar 

  5. Cunnane G, Fitzgerald O et al. Synovial tissue protease gene expression and joint erosions in early rheumatoid arthritis. Arthritis Rheum 2001; 44(8): 1744-1753.

  6. Bluteau G, Conrozier T et al. Matrix metalloproteinase-1, -3,-13 and aggrecanase-1 and -2 are differentially expressed in experimental osteoarthritis. Biochim Biophys Acta 2001; 1526(2): 147–158.

    Google Scholar 

  7. Massova I, Kotra LP et al. Matrix metalloproteinases: Structures, evolution, and diversification. FASEB J 1998; 12: 1075–1095.

    Google Scholar 

  8. Sanchez-Lopez R, Alexander CM et al. Role of zinc-bindingand hemopexin domain-encoded sequences in the substrate specificity of collagenase and stromelysin-2 as revealed by chimeric proteins. J Biol Chem 1993; 268: 7238–7247.

    Google Scholar 

  9. Gross, J, Lapiere CM. Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci U. S. A. 1962; 48: 1014–1022.

    Google Scholar 

  10. Imbert C, Kauffmann-Lacroix C, Daniault G, Jacquemin JL, Rodier MH. Effect of matrix metalloprotease inhibitors on the 95 kDa metallopeptidase of Candida albicans. J Antimicrob Chemother. 2002 June; 49(6): 1007–1010.

  11. Gopinathan U, Ramakrishna T, Willcox M, Rao CM, Balasubramanian D, Kulkarni A, Vemuganti GK, Rao GN. Enzymatic, clinical and histologic evaluation of corneal tissues in experimental fungal keratitis in rabbits. Exp Eye Res. 2001 April; 72(4): 433–442.

    Google Scholar 

  12. Lovejoy B, Cleasby A et al. Structure of the catalytic domain of fibroblast collagenase complexed with an inhibitor. Science 1994; 263: 375–377.

    Google Scholar 

  13. Zaini F, Moore MK et al. The antigenic composition and protein profiles of eumycetoma agents. Mycoses 1991; 34: 19–28.

    Google Scholar 

  14. Bradford, M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248–254.

    Google Scholar 

  15. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–685. 37

    Google Scholar 

  16. Hudson L, Hay FC. Practical Immunology. Blackwell Scientific Publications, Oxford, 1991: 65–70.

    Google Scholar 

  17. Heussen C, Dowdle EB. Electrophoretic analysis of plasminogen activator in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. Analytical Biochemistry 1980; 102: 196–202.

    Google Scholar 

  18. Thant AA, Serbulea M et al. c-Ras is required for the activation of the matrix metalloproteinases by concanavalin A in 3Y1 cells. FEBS Letters 1997; 406: 28–30.

    Google Scholar 

  19. Fidler IJ. Molecular biology of cancer: invasion and metastasis. In Cancer: Principles and Practice of Oncology. 5th ed. Philadelphia: JB Lippincott Co, 1997: 135–152.

    Google Scholar 

  20. Stetler-Stevenson, WG, Hewitt R et al. Matrix metallopro-teinases and tumor invasion: from correlation and causality to the clinic. Semin Cancer Biol 1996; 7: 147–154.

    Google Scholar 

  21. Jones L, Ghaneh P et al. The matrix metalloproteinases and their inhibitors in the treatment of pancreatic cancer. Ann New York Acad Sci 1999; 880: 288–307.

    Google Scholar 

  22. Drummond AH, Bekette P et al. Preclinical and clinical studies of MMP inhibitors in cancer. Ann New York Acad Sci 1999; 878: 228–235.

    Google Scholar 

  23. John A, Tuszynski G. The role of matrix metalloproteinase in tumor angiogenesis and tumor metastasis. Pathol Oncol Res 2001; 7(1): 14–23.

    Google Scholar 

  24. Nguyen M, Arkell J et al. Human endothelial gelatinases and angiogenesis. Int J Biochem Cell Biol 2001; 33(10): 960–970.

    Google Scholar 

  25. Rodier MH, el Moudni B, Kauffmann-Lacroix C, Daniault G, Jacquemin JL. A Candida albicans metallopeptidase degrades constitutive proteins of extracellular matrix. FEMS Microbiol Lett. 1999; 177(2): 205–210.

    Google Scholar 

  26. Inokoshi J, Shiomi K et al. Funalenone, a novel collagenase in-hibitor produced by Aspergillus niger. J Antibiot 1999; 52(12): 1095–1100.

    Google Scholar 

  27. Eberhardt W, Schulze M et al. Glucocorticoid-mediated suppression of Cytokine-induced matrix metalloproteinase-9 expression in Rat mesangial cells: involvment of nuclear factor-kappaB and Ets transcription factors. Mol Endocrinol 2002; 16(8): 1752–1766.

    Google Scholar 

  28. Pross C, Farooq MM et al. Dexamethasone inhibits vascular smooth muscle cell migration via modulation of matrix metalloproteinase activity. J Surg Res 2002; 102(2): 57–62.

    Google Scholar 

  29. Sadowski T, Steinmeyer J. Effects of non-steroidal antiinflammatory drugs and Dexamethasone on the activity and expression of matrix metalloproteinase-1, matrix metalloproteinase-3 and tissue inhibitor of metalloproteinases-1 by bovine articular chondrocytes. Osteoarthritis Cartilage 2001; 9(5): 407–415.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biotechnology, Department of Pathobiology, Tehran University of Medical Sciences, P.O. Box: 14155-6446, Tehran, Iran

    Farshid Saadat, Mohammad Pezeshki & Mohammad Reza Khorramizadeh

  2. Division of Molecular Biology, Department of Medical Mycology & Parasitology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, P.O. Box: 14155-6446, Tehran, Iran

    Kamiar Zomorodian & Sassan Rezaie

Authors
  1. Farshid Saadat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kamiar Zomorodian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mohammad Pezeshki
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sassan Rezaie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mohammad Reza Khorramizadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saadat, F., Zomorodian, K., Pezeshki, M. et al. Inhibitory Effect of Aspergillus fumigatus Extract on matrix metalloproteinases Expression. Mycopathologia 158, 33–37 (2004). https://doi.org/10.1023/B:MYCO.0000038429.46699.ac

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:MYCO.0000038429.46699.ac

Search

Navigation