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Effects of soluble TNF-alpha receptor 1 on apoptosis induced by oxidized LDL in endothelial cells

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Abstract

Apoptosis-inducing agents have been reported to cause rapid shedding of tumor necrosis factor receptor 1 (TNFR1) in endothelial cells (EC). Oxidized LDL (oxLDL) has also been known to induce apoptosis of EC and to inhibit proliferation of EC. In the present study, we show that oxLDL also causes shedding of TNFR1 in EC and that EC transfected with soluble TNFR1 (sTNFR1), which is an extracellular domain of TNFR1, can antagonize the toxicity induced by oxLDL. These results suggest that transfection with the sTNFR1 gene plays a protective role against the injury of EC induced by oxLDL. We speculate therefore that sTNFR1 can be a new strategy for treatment of atherogenesis possibly by preventing shedding of TNFR1.

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Authors and Affiliations

  1. Department of Molecular and Cellular Biology, Division of Molecular and Clinical Gerontology, Medical Institute of Bioregulation, Kyushu University, 4546 Tsurumihara, Beppu, Oita, 874-0838, Japan

    Masahiro Sugano, Keiko Tsuchida & Naoki Makino

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  1. Masahiro Sugano
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  2. Keiko Tsuchida
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  3. Naoki Makino
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Sugano, M., Tsuchida, K. & Makino, N. Effects of soluble TNF-alpha receptor 1 on apoptosis induced by oxidized LDL in endothelial cells. Mol Cell Biochem 258, 57–63 (2004). https://doi.org/10.1023/B:MCBI.0000012836.11573.41

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