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Amplified Fragment Length Polymorphism (AFLP) Reveals Species-Specific Markers in the Daphnia Galeata–HyalinaSpecies Complex

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Abstract

Daphnia often occur in species complexes that consist of two or more co-occurring species and their hybrids. Hybrid individuals are often capable of sexual reproduction and so backcrossing with introgression occurs. To better understand hybridization and backcrossing frequency, we sought to develop PCR-based, species-specific markers in the Daphnia galeata–hyalina species complex using amplified fragment length polymorphism (AFLP). This technique produces large numbers of reproducible markers for assessing diversity across the nuclear genome and provides several advantages over mtDNA and microsatellite approaches. We examined 28 clones of D. galeata, D. hyalina, and their hybrids isolated from Lake Constance on the Swiss-German border. Using a single AFLP primer combination we found five potential species-specific markers, defined as bands that occurred in >80% of one parental species and <20% of the other. Two bands appeared to be co-dominant and were present (homozygous) in D. galeata, absent in D. hyalina, and heterozygous in the hybrid. We conclude AFLP could provide enough PCR-based, species-specific markers to identify species, hybrids, and backcrosses from even small amounts of tissue (i.e. resting eggs).

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Authors and Affiliations

  1. Department of Limnology, EAWAG, Überlandstrasse 133, Postfach 611, 8600, Dübendorf, Switzerland

    Massimiliano Gili, Michael T. Monaghan & Piet Spaak

  2. Via Stazione 78, 10060, None (Torino), Italy

    Massimiliano Gili

  3. Molecular Systematics Laboratory, Department of Entomology, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK

    Michael T. Monaghan

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  1. Massimiliano Gili
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  2. Michael T. Monaghan
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  3. Piet Spaak
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Gili, M., Monaghan, M.T. & Spaak, P. Amplified Fragment Length Polymorphism (AFLP) Reveals Species-Specific Markers in the Daphnia Galeata–HyalinaSpecies Complex. Hydrobiologia 526, 63–71 (2004). https://doi.org/10.1023/B:HYDR.0000041613.30678.56

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