Skip to main content
SpringerLink
Log in

A Yeast DNA Microarray for the Evaluation of Toxicity in Environmental Water Containing Burned Ash

  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract

Numerous studies on the hazard assessment and epidemiological healthresponses to burned ash have been reported. However, there is littleinformation on the potential toxicity of unknown chemical complexesin burned ash. For an overall evaluation of the multiple toxicitiesof burned ash, a DNA microarray was used in this study, as a new attempt to assess these toxicities. Using the global gene expression on yeast DNA chip to reflect the changes in mRNA levels, our study discovered a lot of evidences for the action of cell homeostasis and stress response etc., against the toxic effects on yeast cells. On the genes of 5,117 open reading frames(ORFs), as valid spots in a microarray, 997 were up-regulated, 1,259 were down-regulated and 2,861 remained unchanged. A detailed analysis of the microarray revealed the genes that were dynamically correlated to the function of the subcellular localization, energy/metabolism, various stress responses/cell homeostasis and detoxification. Significantly, the toxicities, caused by reactive oxygen species(ROS), metals and the other xenobiotics, were indicated in burned ash. Also, the possibility of mutagenicity of the burned ash was suggested on the basis of the DNA repair related genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Behnisch, P. A., Hosoe, K., Shiozaki, K., Ozaki, H., Nakamura, K. and Sakai S.: 2002, 'Lowtemperature thermal decomposition of dioxin-like compounds in fly ash: combination of chemical analysis with in vitro bioassays (EROD and DR-CALUX)', Environ. Sci. Technol. 36(23), 5211–5217.

    PubMed  Google Scholar 

  • Choi, J. H., Lou, W. and Vancura A.: 1998, 'A novel membrane-bound glutathione S-transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae', J. Biol. Chem. 273(45), 29915–29922.

    PubMed  Google Scholar 

  • De Virgilio, C., Hottiger, T., Dominguez, J., Boller, T. and Wiemken, A., 1994, 'The role of trehalose synthesis for the acquisition of thermotolerance in yeast', Eur. J. Biochem. 219, 179–186.

    PubMed  Google Scholar 

  • Delaunay, A., Isnard, A. D. and Toledano, M.: 2000, 'H2O2 sensing through oxidation of the Yap1 transcription factor', EMBO J. 19, 5157–5166.

    PubMed  Google Scholar 

  • Diabate, S., Mulhopt, S., Paur, H. R., Wottrich, R. and Krug, H. F.: 2002, 'In vitro effects of incinerator fly ash on pulmonary macrophages and epithelial cells', Int. J. Hyg. Environ. Health 204(5-6), 323-326

    Google Scholar 

  • Eisen, M. B., Spellman, P. T., Brown, P. O. and Botstein D.: 1998, 'Cluster analysis and display of genome-wide expression patterns', Proc. Natl. Acad. Sci. 95(25), 14863–14868

    PubMed  Google Scholar 

  • Fujita, K., Iwahashi, H., Kawai, R. and Komatsu, Y.: 1998, 'Hsp 104 expression and morphological changes associated with disinfectants in environmental bioassay using stress response', Water Sci. Technol. 38, 237–243.

    Google Scholar 

  • Guthrie, C.: 1990, 'Guide to Yeast Genetics and Molecular Biology', in G. R. Funk (ed.), Methods in Enzymology, Academic, San Diego, CA, U.S.A., pp. 398–401

    Google Scholar 

  • Hohmann, S.: 2002, 'Osmotic stress signaling and osmoadaptation in yeast', Microbiol. Mol. Biol. Rev. 66(2), 300–372.

    PubMed  Google Scholar 

  • Ishikawa, T.: 1992, 'The ATP-dependent glutathione S-conjugate export pump', Trends Biochem. Sci. 17(11), 463–468.

    PubMed  Google Scholar 

  • Jensen, L. T., Howard, W. R., Strain, J. J., Winge, D. R. and Culotta, V. C.: 1996, 'Enhanced effectiveness of copper ion buffering by CUP1 metallothionein compared with CRS5 metallothionein in Saccharomyces cerevisiae', J. Biol. Chem. 271(31), 18514–18519.

    PubMed  Google Scholar 

  • Kille, P., Hemmings, A. and Lunney, E. A.: 1994, 'Memories of metallothionein',Biochim. Biophys. Acta 1205(2), 151–161

    PubMed  Google Scholar 

  • Kitagawa, E., Takahashi, J., Momose, Y. and Iwahashi, H.: 2002, 'Effects of the pesticide thiuram: genome-wide screening of indicator genes by yeast DNA microarray', Environ. Sci. Technol. 36(18), 3908–3915.

    PubMed  Google Scholar 

  • Koerkamp, M. G., Rep, M. and Bussemaker, H. J. et al.: 2002, 'Dissection of transient oxidative stress response in Saccharomyces cerevisiae by using DNA microarrays', Mol. Biol. Cell. 13(8), 2783–2794.

    PubMed  Google Scholar 

  • Lesuisse, E. and Labbe, P.: 1995, 'Effects of cadmium and of YAP1 and CAD1/YAP2 genes on iron metabolism in the yeast Saccharomyces cerevisiae', Microbiology 141(Pt 11), 2937–2943.

    PubMed  Google Scholar 

  • Mager, W. H. and Moradas-Ferreira, P.: 1993, 'Stress response of the yeast', Biochem. J. 290, 1–13.

    PubMed  Google Scholar 

  • Mager, W. H. and Varela, J. C. S.: 1993, 'Osmostress response of the yeast Saccharomyces cerevisiae', Mol. Microbiol. 10, 253–258.

    PubMed  Google Scholar 

  • McClellan, R. O.: 2002, 'Setting ambient air quality standards for particulate matter', Toxicol. 27(181-182), 329–347.

    Google Scholar 

  • Momose, Y. and Iwahashi, H.: 2001, 'Bioassay of cadmium using a DNA microarray genome-wide expression patterns of Saccharomyces cerevisiae response to cadmium', Environ. Toxicol. Chem. 20(10), 2353–2360.

    PubMed  Google Scholar 

  • Moradas-Ferreira, P., Costa, V., Piper, P. and Mager, W.: 1996, 'The molecular defenses against reactive oxygen species in yeast', Mol. Microbiol. 19(4), 651–658.

    PubMed  Google Scholar 

  • Nakajima-Shimada, J., Sakaguchi, S., Tsuji, F. I., Anraku, Y. and Iida, H.: 2000, 'Ca2+ signal is generated only once in the mating pheromone response pathway in Saccharomyces cerevisiae', Cell. Struct. Funct. 25(2), 125–131.

    PubMed  Google Scholar 

  • Prahalad, A. K., Inmon, J., Dailey, L. A., Madden, M. C., Ghio, A. J. and Gallagher, J. E.: 2001, 'Air pollution particles mediated oxidative DNA base damage in a cell free system and in human airway epithelial cells in relation to particulate metal content and bioreactivity', Chem. Res. Toxicol. 14(7), 879–887.

    PubMed  Google Scholar 

  • Quay, J. L., Reed, W., Samet, J. and Devlin, R. B.: 1998, 'Air pollution particles induce IL-6 gene expression in human airway epithelial cells via NF-kappaB activation', Am. J. Respir. Cell. Mol. Biol. 19(1), 98–106.

    PubMed  Google Scholar 

  • Silbajoris, R., Ghio, A. J., Samet, J. M., Jaskot, R., Dreher, K. L. and Brighton, L. E.: 2000, 'In vivo and in vitro correlation of pulmonary MAP kinase activation following metallic exposure', Inhal. Toxicol. 12(6), 453–468.

    PubMed  Google Scholar 

  • Varela, J. C., Praekelt, U. M., Meacock, P. A., Planta, R. J. and Mager, W. H.: 1995, 'The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A', Mol. Cell. Biol. 15(11), 6232–6245.

    PubMed  Google Scholar 

  • Vido, K., Spector, D., Lagniel, G., Lopez, S. and Toledano, M.: 2001, 'A proteome analysis of the cadmium response in Saccharomyces cerevisiae', J. Biol. Chem. 276, 8469–8474.

    PubMed  Google Scholar 

  • Volkert, M. R., Elliott, N. A. and Housman, D. E.: 2000, 'Functional genomics reveals a family of eukaryotic oxidation protection genes', Proc. Natl. Acad. Sci. 97(26), 14530–14535.

    PubMed  Google Scholar 

  • Werner, M., Braun, E., Johnston, G. C. and Singer, R. A.: 1993, 'Stationary phase in the yeast Saccharomyces cerevisiae', Microbiol. Rev. 57, 383–401.

    PubMed  Google Scholar 

  • Zahringer, H., Holzer, H. and Nwaka, S.: 1998, 'Stability of neutral trehalose during heat stress in Saccharomyces cerevisiae is dependent on the activity of the catalytic subunits of campdependent protein kinase Tpk2 and Tpk2', Eur. J. Biochem. 255, 544–551.

    PubMed  Google Scholar 

  • Zuk, D., Belk, J. P. and Jacobson A., 1999, 'Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover', Genetics 153(1), 35–47.

    PubMed  Google Scholar 

Download references

Authors
  1. Hyun J. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Ishidou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Kitagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y. Momose
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Iwahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, H.J., Ishidou, E., Kitagawa, E. et al. A Yeast DNA Microarray for the Evaluation of Toxicity in Environmental Water Containing Burned Ash. Environ Monit Assess 92, 253–272 (2004). https://doi.org/10.1023/B:EMAS.0000014504.03500.41

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:EMAS.0000014504.03500.41

Search

Navigation