Abstract
Probiotics are characterized by their ability to interact with commensal microflora in the gastrointestinal tract to produce beneficial health effects. In vitro studies suggest that Lactobacillus species have the potential to suppress the growth of Helicobacter pylori. The goal of this study was to determine if pretreatment of mice with a commercial mixture of live probiotics (L. rhamnosus, strain R0011, and L. acidophilus, strain R0052) would suppress colonization of H. pylori, strain SS1. Thirty C57BL/6 female mice were divided into four groups: Group A was fed sterile water, group B received probiotics in sterile drinking water, group C was challenged orogastrically with H. pylori, and group D was pretreated with probiotics in drinking water prior to and following challenge with H. pylori. Rectal swabs, stomach homogenates, and luminal contents from ileum and colon were plated onto colistin nalidixic acid plates. Serial dilutions of stomach homogenates were plated onto H. pylori-sensitive agar plates and incubated under microaerophilic conditions. Tissue samples from the stomach were analyzed histologically to determine the degree of H. pylori colonization, mucosal inflammation, and epithelial cell apoptosis. Probiotics in drinking water did not affect the overall well-being of mice. Lactobacillus species were excreted in stools over the entire duration of treatment. Pretreatment with probiotics reduced the number of mice with H. pylori growth from stomach homgenates (100 to 50%; P = 0.02). The percentage of mice with moderate-severe H. pylori-induced inflammation in the gastric antrum was reduced with probiotic pretreatment (71 to 29%; P = 0.14). However, pretreatment with probiotics did not prevent H. pylori-induced apoptosis in the gastric mucosa. This preparation of probiotics provided a safe and novel approach for reducing H. pylori colonization and bacterial-induced inflammation of mice.
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REFERENCES
Metchnikoff E: The prolongation of life: optimistic studies. InC Mitchell (ed). Prolongation of Life. London, William Heinemann, 1907, pp 161–183
Guarner F, Malagelada JR: Gut flora in health and disease. Lancet 361(9356):512–519, 2003
Prajapati JB, Nair BM: The history of fermented foods. In Handbook of Fermented Foods. ER Farnworth (ed). Boca Raton, FL, CRC Press, 2003, pp 1–25
Gionchetti P, Rizzello F, Helwig U, Venturi A, Lammers KM, Brigidi P, Vitali B, et al.: Prophylaxis of pouchitis onset with probiotic therapy: A double-blind, placebo controlled trial. Gastroenterology 124:1202–1209, 2003
Marteau PR, de Vrese M, Cellier CJ, Schrezenmeir J: Protection from gastrointestinal diseases with the use of probiotics. Am J Clin Nutr 73 (Suppl2):430S–436S, 2001
Huang, JS, Athos B; Lee JW, Diaz A, Davidson EJ: Efficacy of probiotic use in acute diarrhea in children: A meta-analysis. Dig Dis Sci 47(11):2625–2634, 2002
Van Neil CW, Feudtner C, Garrison MM, ChristakisDA: Lactobacillus therapy for acute infectious diarrhea in children:Ameta-analysis. Pediatrics 109:678–684, 2002
Suerbaum S, Michetti P: Helicobacter pyloriinfection. N Engl J Med 347:1175–1186, 2002
NIH Consensus Development Panel on Helicobacter pyloriin Peptic Ulcer Disease. JAMA 272:65, 1994
Covacci A, Censini S, Bugnoli M, Petracca R, Burroni D, Macchia G, Masson A, et al.: Helicobacter pylori, a paradigm for chronic mucosal inflammation and prevention. Adv Intern Med 41:85–117, 1996
Chatterjee A, Yasmin T, Bagchi D, Stohs S: The bactericidal effects of Lactobacillus acidophilus, garcinol and Protykin®?compared to clarithromycin, on Helicobacter pylori.Mol Cell Biol 243:29–35, 2003
Johnson-Henry K, Tompkins T, Sherman P: Effect of lactobacillus on enterohemorrhagic Esherichia coli-induced alterations in intestinal epithelial cell permeability. Annu Meet Am Soc Microbiol, Orlando, FL, 2001, abstr B-188
Lee A, O'Rourke J, Ungaria MC, Robertson B, Daskalopoulos G, Dixon MF: A standardized mouse model of Helicobacter pyloriinfection: Introducing the Sydney strain. Gastroenterology 112:1386–1397, 1997
Jones N, Day A, Jennings HA, Sherman PM: Helicobacter pyloriinduces gastric epithelial cell apoptosis in association with increased Fas receptor expression. Infect Immun 67:4237–4242, 1999
Schillinger U, Lucke FK: Antibacterial activity of Lactobacillus sakeisolated from meat. Appl Environ Microbiol 55(8):1901–1906, 1989
Cantey J, Hosterman D: Characterization of colonization of the rabbit gastrointestinal tract by Escherichia coliRDEC-1. Infect Immun 26(3):1099–1103, 1979
Jones N, Day A, Jennings HA, Shannon PT, Galindo-Mata E, Sherman PM: Enhanced disease severity in Helicobacter pyloriinfected mice deficient in Fas signaling. Infect Immun 70(5):2591–2597, 2002
P. Sutton, Wilson J, Lee A: Further development of the Helicobacter pylorimouse vaccination model. Vaccine 18:2677–2685, 2000
Drumm B, Sherman P, Cutz E, Karmali M: Association of Campylobacter pylorion the gastric mucosa with antral gastritis in children. N Engl J Med 316:1557–1561, 1987
Jax Notes: Malocculusion in the laboratory mouse. www.jax.org/ jaxmice
Mercenier A, Pavan S, Pot B: Probiotics as biotherapeutic agents: Present knowledge and future prospects. Curr Pharm Design 8:99–110, 2002
Tomasik PJ, Tomasik P: Probiotics and prebiotics: Review. Cereal Chem 80:113–117, 2003
Kocian J: Lactobacilli in the treatment of dyspepsia in dysmicrobia of various aetiology. Vnitrni Lekarstvi 40:79–83, 1994
Bielanski W, Ziemniak W, Plonka M, Dobrzanska MJ, Kaminska A, Konturek SJ: Improvement of anti-Helicobacter pyloritherapy by the use of commercially available probiotics. Gut 51(11):A98, 2002
Hamilton-Miller JMT: The role of probiotics in the treatment and prevention of Helicobacter pyloriinfection. Int J Antimicrobial Agents 22:360–366, 2003
Brzozowski T, Konturek P.C, Bielanski W, Kwiecien S, Drozdowicz D, Pajdo R, Konturek SJ: Effect of probiotics and triple eradication therapy on the cyclooxygenase (COX)-2 expression and functional impairment in Helicobacter pylori(HP)-infected Mongolian gerbils. Annual AGA-DDW meeting, Orlando, FL, 2003, abstr M1501
Fernandez MF, Boris S, Barbes C: Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract. J Appl Microbiol 94(3):449–455, 2003
Saito N, Lpmosjo L, Sato F, Kato M, Takeda H, Sagiyama T, Asaka M: Plural transformation-processes from spiral to coccoid Helicobacter pyloriand its viability. J Infect 46(1):49–55, 2003
Stingl K, Uhlemann EM, Deckers-Hebestreit G, Schmid R, Bakker EP, Altendorf K: Prolonged survival and cytoplasmic pH homeostasis of Helicobacter pyloriat pH1. Infect Immun 69:1178–11180, 2001
Sachs G, Weeks DL, Melchers K, Scott DR: The gastric biology of Helicobacter pylori.Annu Rev Phisiol 65:349–369, 2003
Sgouras D, Maragkoudakis P, Petraki D, Martinez-Gonzalez B, Eriotou E, Michopoulos S, Kalantzopoulos G, Tsakalidou E, Mentis A: In vitroand in vivoinhibition of Helicobacter pyloriby Lactobacillus caseistrain Shirota. Appl Environ Microb 70:518–526, 2004
Mangell P, Nejdfors P, Wang M, Ahrne S, Westrom B, Thorlacius H, Jeppsson B: Lactobacillus plantarum299v inhibits Escherichia coli-induced intestinal permeability. Dig Dis Sci 47(3):511–516, 2002
Fried LF, Palevsky PM: Renal disease. Med Clin NoAm 81(3):585–609, 1997
De Waard R, Snel J, Bokken GC, Tan PS, Schut F, Huis In't Veld JH: Comparison of faecal Lactobacillus populations in experimental animals from different breeding facilities and possible consequences for probiotic studies. Left Appl Microbiol 34(2):105–109, 2002
Dieleman L, GoerresM, Arends A, Sprengers D, Torrice C, Hoentjen F, Grenther WB, et al.: LactobacillusGG prevents recurrence of colitis in HLA-B27 transgenic rats after antibiotic treatment. Gut 52(3):370–376, 2003
Yuki N, Shimazaki T, Kushiro A, Watanabe K, Uchida K, Yuyama T, Morotomi M: Colonization of the stratified squamous epithelium of the nonsecreting area of the horse stomach by lactobacilli. Appl Environ Microbiol 66(11):5030–5034, 2000
Wu JJ, Lo CY, Wu HW, Chen JH, Lin YS, Lin MD: Impact of supplement with Lactobacillusand Bifidobacterium-containing yogurt on triple therapy for Helicobacter pylorieradication. Aliment Pharmacol Ther 16:1669–1675, 2002
Isolauri E: Probiotics for infectious diarrheoea. Gut 52:436–437, 2003
Bezkorovainy A: Probiotics: Determinants of survival and growth in the gut. Am J Clin Nutr 73:399S–405S, 2001
Resta-Lenert S, Barrett K: Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive Escherichia coli(EIEC). Gut 52:988–997, 2003
Mack DR, Michail S, Wei S, McDougall L, Hollingsworth MA: Probiotics inhibit enteropathogenic E. coliadherence in vitroby inducing intestinal mucin gene expression. AmJ Physiol 276;G941–G950, 1999
Johnson-Henry K, Tompkins TA, Sherman PM: Lactobacillus species inhibit adherence of enteropathogenic Escherichia coliand enterohemorrhagic E. colito host epithelial cells. Annual meeting of the Danone Symposium on Fermented Food, Fermentation and Intestinal Flora, New York, 2000, abstr 7
Sutton P, Wilson J, Lee A: Further development of Helicobacter pylorimouse vaccination model. Vaccine 18:2677–2685, 2000
Garhart CA, Redline RW, Nedrud JG, Czinn SJ: Clearance of Helicobacter pyloriinfection and resolution of postimmunization gastritis in a kinetic study of prophylactically immunized mice. Infect Immun 70:3529–3538, 2002
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Johnson-Henry, K.C., Mitchell, D.J., Avitzur, Y. et al. Probiotics Reduce Bacterial Colonization and Gastric Inflammation in H. pylori-Infected Mice. Dig Dis Sci 49, 1095–1102 (2004). https://doi.org/10.1023/B:DDAS.0000037794.02040.c2
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DOI: https://doi.org/10.1023/B:DDAS.0000037794.02040.c2