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Fractionation of Trichoderma Reesei Cellulases by Hydrophobic Interaction Chromatography on Phenyl-Sepharose

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Abstract

Trichoderma reesei cellulase complex was fractionated using hydrophobic interaction chromatography with a phenyl-Sepharose column. Using a linear gradient of ammonium sulphate in the eluent buffer, a selective separation of endoglucanases was obtained at 15 °C with a four-fold increase in specific activity.

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Authors and Affiliations

  1. Department of Chemistry, University of Beira Interior, 6201-001, Covilhã, Portugal

    C^andida T. Tomaz & João A. Queiroz

Authors
  1. C^andida T. Tomaz
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  2. João A. Queiroz
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Tomaz, C.T., Queiroz, J.A. Fractionation of Trichoderma Reesei Cellulases by Hydrophobic Interaction Chromatography on Phenyl-Sepharose. Biotechnology Letters 26, 223–227 (2004). https://doi.org/10.1023/B:BILE.0000013719.92024.04

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  • DOI: https://doi.org/10.1023/B:BILE.0000013719.92024.04

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