Skip to main content
SpringerLink
Log in

Successful Hybridization of Acipenser Species Using Cryopreserved Sperm

  • Published:
Aquaculture International Aims and scope Submit manuscript

Abstract

Successful hybridization of sterlet (Acipenser ruthenus) × Siberian sturgeon (Acipenser baeri), sterlet × Russian sturgeon (Acipenser gueldenstaedti) and sterlet × European sturgeon (Acipenser sturio) was carried out for the first time using cryopreserved semen of sturgeon males and sterlet × sterlet crosses as controls. Sperm of all three species was diluted with a cryodiluent composed of 23.4 mM sucrose, 0.25 mM KCl, 30 mM Tris (pH 8.0) and 10% methanol. The samples were frozen in plastic straws in the vapor of liquid nitrogen at the height of 3 cm above the level of nitrogen for 3 min. Following thawing approximately 3000 sterlet eggs were fertilized with six straws of frozen-thawed sperm. The hatching rate with sterlet sperm was 30.6% while the hatching rate of A. ruthenus × A. baeri, A. ruthenus × A. gueldenstaedti and A. ruthenus × A. sturio hybrids was 50, 17.4 and 34%, respectively. Morphometric markers as well as random amplified polymorphic DNA (RAPD) assay was used to verify interspecific hybridization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alarcon, J.A. and Alvarez, M.C. 1999. Genetic identification of sparid species by isozyme markers: application to interspecific hybrids. Aquaculture 173: 95-103.

    Google Scholar 

  • Bercsényi, M., Magyary, I., Urbányi, B., Orbán, L. and Horváth, L. 1998. Hatching out gold-fish from common carp eggs: interspecific androgenesis between two cyprinid species. Genome 41: 573-579.

    Google Scholar 

  • Billard, R. and Lecointre, G. 2001. Biology and conservation of sturgeon and paddlefish. Reviews in Fish Biology and Fisheries 10: 355-392.

    Google Scholar 

  • Birstein, B.J. and DeSalle, R. 1998. Molecular phylogeny of Acipenserinae. Molecular Phylogeny and Evolution 9: 141-155.

    Google Scholar 

  • Chebanov, M. and Billard, R. 2001. The culture of sturgeons in Russia: production of juveniles for stocking and meat consumption. Aquatic Living Resources 14: 375-381.

    Google Scholar 

  • Cherepanov, V.V., Drokin, S.I., Ochkur, S.I., Dzuba, B.B., Chikhachov, A.S. and Kopeika, E.F. 1993. Freezing of sperm of the Azov-Black Sea Acipenserids. International Symposium on Sturgeons, 6-11 September 1993, Moscow-Kostroma-Moscow, Abstract Bulletin, p. 63.

  • Ciereszko, A., Toth, G.P., Christ, S.A. and Dabrowski, K. 1996. Effect of cryopreservation and theophylline on motility characteristics of lake sturgeon (Acipenser fulvescens) spermatozoa. Theriogenology 45: 665-672.

    Google Scholar 

  • Dettlaff, T.A., Ginsburg, A.S. and Schmalhausen, O.I. 1993. Sturgeon Fishes, Developmental Biology and Aquaculture. Springer-Verlag, Berlin, Heidelberg.

    Google Scholar 

  • Drokin, S.I., Cherepanov, V.V., Kopeika, E.F. and Shilin, N.I. 1991. Sakhalin sturgeon: how to preserve gene fund. Rybnoe Khoziaistvo 7: 38-39 (in Russian).

    Google Scholar 

  • Drokin, S.I., Cherepanov, V.V., Kopeika, E.F. and Shilin, N.I. 1993. Cryopreservation of sperm of Sakhalin sturgeon (Acipenser medirostris micadoi): problems and prospects for cryopreserved sperm collection from rare and endangered sturgeon species. International Symposium on Sturgeons, 6-11 September 1993, Moscow-Kostroma-Moscow, Abstract Bulletin, p. 64.

  • Elo, K., Ivanoff, S., Vuorinen, J.A. and Piironen, J. 1997. Inheritance of RAPD markers and detection of interspecific hybridization with brown trout and European salmon. Aquaculture 152: 55-65.

    Google Scholar 

  • Glogowski, J., Kolman, R., Szczepkowski, M., Horváth, A., Urbányi, B., Sieczyński, P., Rzemieniecki, A., Domaga?a, J., Demianowicz, W., Kowalski, R. and Ciereszko, A. 2002. Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture 211: 367-373.

    Google Scholar 

  • Horváth, A. and Urbányi, B. 2000. Cryopreservation of sterlet (Acipenser ruthenus) sperm. In: Norberg, B., Kjesbu, O.S., Taranger, G.L., Andersson, E. and Stefansson, S.O. (Eds.), Proceedings of the 6th International Symposium on Reproductive Physiology of Fish, Bergen, Norway, 4-9th July 1999. Bergen, Norway, p. 441.

  • Jähnichen, H., Warnecke, D., Trölsch, E., Kohlmann, K., Bergler, H. and Pluta, H.-J. 1999a. Motility and fertilizing capability of cryopreserved Acipenser ruthenus L. sperm. Journal of Applied Ichthyology 15: 204-206.

    Google Scholar 

  • Jähnichen, H., Kohlmann, K. and Rennert, B. 1999b. Juvenile growth of Acipenser ruthenus and 4 different sturgeon hybrids. Journal of Applied Ichthyology 15: 248-249.

    Google Scholar 

  • Kolman, R., Szczerpowski, M. and Pyka, J. 1997. Evaluation of the Siberian sturgeon (Acipenser baeri Brandt) and green sturgeon (A. medirostris Ayres) hybrid comparing to the mother species. Archives of Polish Fisheries 5: 51-58.

    Google Scholar 

  • Krylova, V.D. 1980a. Variability and inheritance of traits in the first and second generation of beluga-sterlet hybrids Huso huso L. × Acipenser ruthenus L. in connection with selection work. Voprosy Ikhtiologii 20: 232-247.

    Google Scholar 

  • Krylova, V.D. 1980b. Morphometric description of hybrids of beluga Huso huso L. and sevryuga Acipenser stellatus Pallas. Voprosy Ikhtiologii 20: 875-882.

    Google Scholar 

  • Liu, Z.J., Li, P., Argue, B.J. and Dunham, R.A. 1999. Random amplified polymorphic DNA markers: usefulness for gene mapping and analysis of genetic variation of catfish. Aquaculture 174: 59-68.

    Google Scholar 

  • Liu, S., Liu, Y., Zhou, G., Zhang, X., Luo, C., Feng, H., He, X., Zhu, G. and Yang, H. 2001. The formation of tetraploid stocks of red crucian carp×common carp hybrids as an effect of interspecific hybridization. Aquaculture 192: 171-186.

    Google Scholar 

  • Müller, H. 1983. Fische Europas. Neumann Verlag, Leipzig 500 p. (in German).

    Google Scholar 

  • Nikoljukin, N.I. 1964. Some observations on the histological structure of gonads in hybrids of Acipenseridae. Federal Scientific Research Institute of Marine Fish Culture and Oceanography (VNIRO) LV, 145-158 (in Russian).

  • Ohara, K., Ariyoshi, T., Sumida, E., Sitizyo, K. and Taniguchi, N. 2000. Natural hybridization between diploid crucian carp species and genetic independence of triploid crucian carp elucidated by DNA markers. Zoological Science 17: 357-364.

    Google Scholar 

  • Pintér, K. 1989. Fishes of Hungary. Akadémiai Kiadó, Budapest (in Hungarian).

    Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning. CSH Laboratory Press, Cold Spring Harbor, New York, 350 p.

    Google Scholar 

  • Tsvetkova, L.I., Cosson, J., Linhart, O. and Billard, R. 1996. Motility and fertilizing capacity of fresh and frozen-thawed spermatozoa in sturgeons Acipenser baeri and A. ruthenus. Journal of Applied Ichthyology 12: 107-112.

    Google Scholar 

  • Veselov, E.A. 1977. Freshwater Fishes of the Fauna of the USSR. Prosveshchenie, Moscow, 250 p.

    Google Scholar 

  • Werner, O., Gomez, P.S., Guerra, J. and Martinez, J.F. 2001. Identification of Pistacia × saportae Burnat (Anacardiaceae) by RAPD analysis and morphological characters. Scientia Horticulturae 91: 179-186.

    Google Scholar 

  • Wilkins, N.P., Gosling, E., Curatolo, A., Linnane, A., Jordan, C. and Courtney, H.P. 1995. Fluctuating asymmetry in European salmon, European trout and their hybrids, including triploids. Aquaculture 137: 77-85.

    Google Scholar 

  • Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 31-35.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Fish Culture, Faculty of Agriculture and Environmental Sciences, Szent Istvan University, Páter K. u. 1., Gödöllö, H-2103, Hungary

    Béla Urbányi & Ákos Horváth

  2. Szent-Györgyi Albert ut 4, Agricultural Biotechnology Center, Gödöllö, H-2100, Hungary

    Balázs Kovács

Authors
  1. Béla Urbányi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ákos Horváth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Balázs Kovács
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Béla Urbányi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Urbányi, B., Horváth, Á. & Kovács, B. Successful Hybridization of Acipenser Species Using Cryopreserved Sperm. Aquaculture International 12, 47–56 (2004). https://doi.org/10.1023/B:AQUI.0000017186.59304.6d

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:AQUI.0000017186.59304.6d

Search

Navigation