biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 46:13-19, 2003 | DOI: 10.1023/A:1027364427795

In vitro Plant Regeneration of Melia azedarach L.: Shoot Organogenesis from Leaf Explants

S.K. Vila1, A.M. Gonzalez1, H.Y. Rey1, L.A. Mroginski1
1 Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste, Corrientes, Argentina

In vitro regeneration of Melia azedarach L. was studied. Shoots were regenerated from calli initiated from leaflets of in vitro growing plants. The best medium for establishment of cultures was Murashige and Skoog (MS) medium with 4.44 μM benzylaminopurine (BAP) + 0.46 μM kinetin (KIN) + 16.29 μM adenine sulphate (ADE). Regenerated shoots were multiplied in MS + 0.44 μM BAP + 0.37 μM KIN + 3.26 μM ADE. Maximal rooting of 89 % was achieved by culture of regenerated shoots in MS + 12.26 μM indole-3-butyric acid for 3 d and subsequently in MS lacking growth regulators for 27 d. Rooted shoots were acclimatized and successfully transferred to soil.

Keywords: auxin; cytokinin; gibberellic acid; leaf culture; rooting
Subjects: auxins; cytokinins; gibberellins; in vitro culture, acclimation from in vitro to ex vitro culture; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis; Melia azedarach; rooting in vitro

Published: July 1, 2003  Show citation

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Vila, S.K., Gonzalez, A.M., Rey, H.Y., & Mroginski, L.A. (2003). In vitro Plant Regeneration of Melia azedarach L.: Shoot Organogenesis from Leaf Explants. Biologia plantarum, 46(1), 13-19. doi: 10.1023/A:1027364427795
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