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Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm

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Abstract

The low density, detergent-insoluble membrane fraction (LD-DIM), where gangliosides are likely to be highly enriched, was prepared from sperm of two sea urchin species, Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus. Immunoblotting showed the presence in the LD-DIM of two receptors for egg ligands, a glycosylphosphatidylinositol (GPI)-anchored protein, and four proteins which may be involved in signal transduction. Co-immunoprecipitation revealed that at least three proteins, the speract receptor, the 63[emsp4 ]kDa GPI-anchored protein and the α subunit of a heterotrimeric Gs protein, are localized in the LD-DIM. This suggests that the LD-DIM fraction may be a membrane microdomain for speract–speract receptor interaction, as well as the subsequent signal transduction pathway involved in induction of sperm respiration, motility and possibly the acrosome reaction.

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Authors and Affiliations

  1. Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464–8601, Japan

    Kaoru Ohta, Chihiro Sato, Tsukasa Matsuda & Ken Kitajima

  2. Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Ohya, Shizuoka, 422–8529, Japan

    Masaru Toriyama

  3. Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California, 92093–0202, U.S.A

    Victor D. Vacquier

  4. Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, New York, 11794–5215, U.S.A

    William J. Lennarz

Authors
  1. Kaoru Ohta
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  2. Chihiro Sato
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  3. Tsukasa Matsuda
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  4. Masaru Toriyama
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  5. Victor D. Vacquier
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  6. William J. Lennarz
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  7. Ken Kitajima
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Ohta, K., Sato, C., Matsuda, T. et al. Co-localization of receptor and transducer proteins in the glycosphingolipid-enriched, low density, detergent-insoluble membrane fraction of sea urchin sperm. Glycoconj J 17, 205–214 (2000). https://doi.org/10.1023/A:1026589223811

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