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Application of solvent engineering to optimize lipase-catalyzed 1,3-diglyacylcerols by mixture response surface methodology

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Abstract

1,3-Diacylglycerol has been introduced in Japan as a cooking oil under the trade name of Econa to reduce body fat accumulation. Solvent engineering was applied to determine the optimum solvent mixtures for the lipase-catalyzed synthesis of 1,3-DAG by mixture response surface methodology. n-Hexane was required to maintain the lipase activity and the product selectivity could be adjusted by changing the hydrophobicity of reaction medium. The optimum yield (∼40%) of 1,3-DAG synthesis was obtained with n-hexane/octane (1:1, v/v).

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Authors and Affiliations

  1. Department of Medical Research, Mackay Memorial Hospital, 45 Minsheng Road, Tamshui, Taipei County, 251, Taiwan

    Hui-Fen Liao

  2. Department of Bioindustry Technology, Dayeh University, 112 Shan-Jiau Road, Da-Tsuen, Chang-Hua, 515, Taiwan

    Wei-Chuan Tsai & Chwen-Jen Shieh

  3. Institute of Bioscience and Biotechnology, National Taiwan Ocean University, 2 Peining Road, Keelung, 202, Taiwan

    Shu-Wei Chang

Authors
  1. Hui-Fen Liao
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  2. Wei-Chuan Tsai
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  3. Shu-Wei Chang
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  4. Chwen-Jen Shieh
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Corresponding author

Correspondence to Chwen-Jen Shieh.

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Liao, HF., Tsai, WC., Chang, SW. et al. Application of solvent engineering to optimize lipase-catalyzed 1,3-diglyacylcerols by mixture response surface methodology. Biotechnology Letters 25, 1857–1861 (2003). https://doi.org/10.1023/A:1026237829284

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  • DOI: https://doi.org/10.1023/A:1026237829284

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