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Functional and structural characterization of anti-β1-adrenoceptor autoantibodies of spontaneously hypertensive rats

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Abstract

Eighteen month old spontaneously hypertensive rats (SHR-rats) showed myocardial dysfunction and autoantibodies directed against the β1-adrenoceptor similarly as known in human dilated cardiomyopathy or Chagas' disease. The agonist-like antibodies were able to activate the β1-adrenoceptor mediated signal transduction cascade in cultured rat cardiomyocytes and induced a long-lasting stimulatory effect resulting in a harmful adrenergic overdrive. The antibodies recognized an epitope of the second extracellular loop of the β1-adrenoceptor identical to that epitope identified in Chagas' disease. In conclusion, our assumption is supported that old SHR-rat are an useful animal model for investigating the role of anti-β1-adrenoceptor antibodies in the induction of human cardiomyopathy.

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Authors and Affiliations

  1. Max Delbruuml;ck Centrum für Molekulare Medizin, Berlin, Germany

    Gerd Wallukat, Svenia Podlowski, Eberhard Nissen & Rosemarie Morwinski

  2. Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany

    Csaba Csonka, Arpad Tosaki & Ingolf E. Blasig

Authors
  1. Gerd Wallukat
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  2. Svenia Podlowski
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  3. Eberhard Nissen
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  4. Rosemarie Morwinski
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  5. Csaba Csonka
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  6. Arpad Tosaki
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  7. Ingolf E. Blasig
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Wallukat, G., Podlowski, S., Nissen, E. et al. Functional and structural characterization of anti-β1-adrenoceptor autoantibodies of spontaneously hypertensive rats. Mol Cell Biochem 251, 67–75 (2003). https://doi.org/10.1023/A:1025469515963

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