Abstract
Twelve-day-old seedlings of Scots pine (Pinus sylvestris L.) were infected with a virulent spore suspension of the fungus Fusarium sporotrichiella var. sporotrichioides Bilai. 72 h after infection, the seedlings were divided into infection-susceptible and infection-resistant ones, and phenolic compounds in the seedling hypocotyls were analyzed. Susceptible seedlings contained 4.3-fold less lignins, 1.4-fold less free and bound proanthocyanidins, 12.7-fold less free hydroxybenzoic acids, 56.6-fold less bound hydroxybenzoic acids, 2-fold less quinic acid, and 5.1-fold less shikimic acid as compared to the resistant seedlings. At the same time, L-phenylalanine content was increased 3-fold and that of L-tyrosine, 1.3-fold. The activity of quinate:NAD(P)+ oxidoreductase and L-phenylalanine ammonia-lyase in the hypocotyls of susceptible seedlings was 2.3-fold and 1.4-fold lower, respectively, whereas the activity of shikimate:NAD(P)+ oxidoreductase remained unchanged.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Belostokov, G.P., Anatomical Structure of the Seedlings of Coniferous Woody Plants, Dokl. Akad. Nauk SSSR, 1963, vol. 48, pp. 712–716.
Kondrashova, N.Yu. and Yatsenko-Khmelevskii, A.A., The Effect of Several Stimulators on the Growth and Differentiation of the Scots Pine Seedlings, Nauchn. Dokl.Vyssh. Shkol., Ser. Biol., 1977, vol. 4, pp. 108–111.
Popova, A.R., Seasonal Dynamics of Bark Structure in Current Year Shoots of Seedlings and Mature Plants, Mater. 1-i Vses. konf. po anatomii rastenii (Proc. 1st All-Union Conf. on Plant Anatomy), Leningrad: Bot. Inst. im. V.L. Komarova Akad. Nauk SSSR, 1984, p. 134.
Hotter, G.S., Elicitor-Induced Oxidative Burst and Phenylpropanoid Metabolism in Pinus radiata Cell Suspension Cultures, Aust. J. Plant Physiol., 1997, vol. 24, pp. 797–804.
Bach, M. and Seitz, H.U., Elicitor-Induced Defense Responses of a Suspension-Cultured Woody Plant (Larix decidua) and Possible Mechanism of Signal Transduction, Can. J. Bot., 1997, vol. 75, pp. 1243–1251.
Shein, I.V., Polyakova, G.G., Zrazhevskaya, G.K., Pashenova, N.V., and Vetrova, V.P., Accumulation of Phenolic Compounds in Conifer Callus Culture in Response to Wood Blue-Stain Fungi, Fiziol. Rast. (Moscow), 2001, vol. 48, pp. 251–256 (Russ. J. Plant Physiol., Engl. Transl.).
Szumowski, B., Increasing the Resistance of Different Provenances of Polish Larch by Means of Tissue Stimulation to Production of Phenolic Substances That Limit the Magnitude of Infection with Lochnellula willkommii (Hahn Ayers) Dharne, Forest Wood Technol., 1995, no. 46, pp. 3–14.
Polyakova, G.G., Vetrova, V.P., Pashenova, N.V., and Osipov, V.I., Role of Proantocyanidines and Lignin in the Defense Response of Siberian Fir to Inoculation with Micromycetes, Fiziol. Rast. (Moscow), 1995, vol. 42, pp. 622–628 (Russ. J. Plant Physiol., Engl. Transl.).
Butenko, R.G., Kul'tura izolirovannykh tkanei i fiziologiya morfogeneza rastenii (Tissue Culture and Physiology of Plant Morphogenesis), Moscow: Nauka, 1964.
Zhuravlev, I.I., Krangauz, R.A., and Yakovlev, V.G., Bolezni lesnykh derev'ev i kustarnikov (Diseases of Forest Trees and Bushes), Moscow: Lesnaya Promyshlennost', 1974.
Laanest, L.V. and Margna, U.V., The Role of Phenylalanine Ammonia-Lyase in Flavonoid Accumulation in Buckwheat Seedlings, Fiziol. Rast. (Moscow), 1972, vol. 19, pp. 1157–1164 (Sov. Plant Physiol., Engl. Transl.).
Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248–254.
Kozarenko, T.G., Zuev, S.N., and Mulyar, N.F., Ionoobmennaya khromatografiya aminokislot (Ion-Exchange Chromatography of Amino Acids), Novosibirsk: Nauka, 1981.
Varma, R., Varma, R.S., and Wardi, A.H., Separation of Aldononitrile Acetate of Neutral Sugars by Gas-Liquid Chromatography and Its Application to Polysaccharides, J. Chromatogr., 1973, vol. 77, pp. 222–227.
Rhodes, M.J.C., The Physiological Significance of Plants Phenolic Compounds, Annu. Proc. Phytochem. Soc. Eur., 1985, vol. 25, pp. 99–117.
Stafford, N.A., Proanthocyanidins and the Lignin Connection, Phytochemistry, 1988, vol. 27, pp. 1–6.
Zaprometov, M.N., Fenol'nye soedineniya (Phenolic Compounds), Moscow: Nauka, 1993.
Osipov, V.I., The Role of the Shikimate Pathway of Phenolic Compound Biosynthesis in Conifers, Doctoral (Biol.) Dissertation, Moscow: Bakh Inst. Biokhimii, 1990.
Osipov, V.I. and Shein, I.V., The Role of Quinine Acid in Lignin Biosynthesis in Scots Pine, Fiziol. Rast. (Moscow), 1990, vol. 37, pp. 518–525 (Sov. Plant Physiol., Engl. Transl.).
Camm, E. and Towers, G., Phenylalanine Ammonia-Lyase, Phytochemistry, 1973, vol. 12, pp. 961–973.
Osipov, V.I. and Shein, I.V., Shikimate Dehydrogenase from the Needles of Scots Pine, Biokhimiya, 1986, vol. 51, pp. 9–15.
Hunter, R.E., Inactivation of Pectic Enzymes by Polyphenols in Cotton Seedlings of Different Ages Infected with Rhyzoctonia solam, Physiol. Plant Pathol., 1974, vol. 4, pp. 151–159.
Hemingway, R.W., McGrow, G.W., and Stanley, J.B., Polyphenols in Ceratocystis minor Infected Pinus taeda: Fungal Metabolites, Phloem and Xylem Phenols, Agric. Food Chem., 1977, vol. 25, pp. 717–722.
Beckman, C.H., Distribution of Phenols in Specialized Cells of Banana Roots, Phytopathology, 1970, vol. 60, pp. 79–82.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shein, I.V., Shibistova, O.B., Zrazhevskaya, G.K. et al. The Content of Phenolic Compounds and the Activity of Key Enzymes of Their Synthesis in Scots Pine Hypocotyls Infected with Fusarium . Russian Journal of Plant Physiology 50, 516–521 (2003). https://doi.org/10.1023/A:1024776924788
Issue Date:
DOI: https://doi.org/10.1023/A:1024776924788